324,151 research outputs found
Le risorse conoscitive ed estetiche della metafora
No full textSi mettono in evidenza i valori conoscitivi e inventivi della metafora sia nell'àmbito delle scienze umane, sia in quello delle scienze della natur
Expression in male and genomic organization of the gene(s) coding for a major protein secreted by the rat seminal vesicle epithelium
No full textDouble strand cDNA copies of lls poly(A)+mRNA purified from adult rat seminal vesicles (RSV), have been cloned in E.coli C600 using the Pst I site of pBR322. Filter hybridization, nucleotide sequence analysis and positive hybridization translation were used to demonstrate that one of the recombinant plasmids obtained (pRSV25) contained a 260 bp long insert coding for a significant part of the precursor to the protein IV present in the RSV secretion. By using labelled pRSV25 DNA we have found that high levels of RSV IV mRNA were present only in the rat seminal vesicle epithelium. The amounts of RSV IV mRNA present in other tissues of the same organism were below the levels detectable by the methods used. In addition, other data reported here indicate that the RSV IV gene(s) is present in both sexes, probably with a different organization
Expression in male and genomic organization of the gene(s) coding for a major protein secreted by the rat seminal vesicle epithelium
No full textDouble strand cDNA copies of lls poly(A)+mRNA purified from adult rat seminal vesicles (RSV), have been cloned in E.coli C600 using the Pst I site of pBR322. Filter hybridization, nucleotide sequence analysis and positive hybridization translation were used to demonstrate that one of the recombinant plasmids obtained (pRSV25) contained a 260 bp long insert coding for a significant part of the precursor to the protein IV present in the RSV secretion. By using labelled pRSV25 DNA we have found that high levels of RSV IV mRNA were present only in the rat seminal vesicle epithelium. The amounts of RSV IV mRNA present in other tissues of the same organism were below the levels detectable by the methods used. In addition, other data reported here indicate that the RSV IV gene(s) is present in both sexes, probably with a different organization
Anti-apoptotic seminal vesicle protein IV inhibits cell-mediated immunity.
No full textThe in vitro effect of seminal vesicle protein IV (SV-IV) on the cytotoxic activity of human natural or acquired cellular immunity has been investigated by standard immunological procedures, a 51Cr-release cytotoxicity assay, and labeled-ligand binding experiments.
The data obtained demonstrate that: (1) fluoresceinated or [125I]-labeled SV-IV binds specifically to the surface of human purified non-adherent monuclear cells (NA-MNC); (2)SV-IV suppresses the cytotoxicity of natural killer (NK) cells against K562 target cells, that of IL-2-stimulated NK (LAK) cells against DAUDI target cells, and that of VEL antigen-sensitized cytotoxic T lymphocytes (CTLs) against VEL target cells; (3) treatment of K562 target cells alone with SV-IV decreases their susceptibility to NK-induced lysis. These findings indicate that the protein SV-IV has a marked in vitro inhibitory effect on NK, LAK and CTL cytotoxicity, providing a better understanding of its immune regulatory functions
Come una tragedia, la Shoah, divenne una metafora
No full textObiettivo dell'articolo è indagare l'uso della metafora della Shoah: in quali condizioni viene usata? Quali sono i meccanismi cognitivi e sociali sottesi al suo utilizzo
In vitro synthesis of different antigens related to the major secretory protein of the rat seminal vesicle epithelium.
No full textPoly/A)+mRNA, prepared from rat seminal vesicles (RSV), was translated in a rabbit reticulocyte cell-free system. Among the translation products, three proteins (A, B and C), immunologically related to a major RSV secretory protein (RSV-IV), were detected. Recombinenta plasmids, harbouring specific cDNA sequences for RSV-IV, were used to positively select the mRNAs for antigens A and B. Phosphorylation sites were mainly detectable in the antigen B
In vitro synthesis of different antigens related to the major secretory protein of the rat seminal vesicle epithelium.
No full textPoly/A)+mRNA, prepared from rat seminal vesicles (RSV), was translated in a rabbit reticulocyte cell-free system. Among the translation products, three proteins (A, B and C), immunologically related to a major RSV secretory protein (RSV-IV), were detected. Recombinenta plasmids, harbouring specific cDNA sequences for RSV-IV, were used to positively select the mRNAs for antigens A and B. Phosphorylation sites were mainly detectable in the antigen B
Protein SV-IV promotes nitric oxide production not associated with apoptosis in murine macrophages.
No full textPhosphorylation of seminal vesicle protein IV on Ser58 enhances its peroxidase-stimulating activity
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