6 research outputs found
Effect of Ferula hormonis Extract on Social Aggression, Fertility and Some Physiological Parameters in Prepubertal Male Mice.
No full textProtective Effect of Phenolic Extract of <i>Urtica dioica</i> leaves against Carbon Tetra-Chloride Induced Hepatotoxicity in Male Rats
No full textAssessing the Effects of Mentha longifolia on Liver Structure in Castrated Rats
No full textThe liver is the largest internal organ in the human body. It has dual roles: An exocrine function, producing bile to aid digestion, and an endocrine-like function, helping regulate metabolism. It cleanses the blood, makes important proteins, and stores essential nutrients. This study aimed to assess the impact of Mentha longifolia extract on a variety of physiological, biochemical, and histological parameters. A total of 21 male rats (Rattus norvegicus), 3 weeks of age, from the order Rodentia and family Muridae, were used in this study. Rats were divided into three groups, each consisting of seven rats. Both Group 1 (control) and Group 2 (castrated) of male rats received tap water. However, male rats in Group 3 were castrated and administered a daily intragastric extract of M. longifolia (6 g/kg). In castrated-treated groups, the effects of consuming a freshly made extract of M. longifolia were examined for various biochemical parameters of liver enzymes and lipid profile, as well as the histological structures of liver tissue. The outcomes of the biochemical tests performed on the treated subjects did not reveal any notable variations. Histological sections of the castrated group’s liver tissue showed cellular inflammation of lymphocytes, ballooning degeneration, and acidophil bodies (Councilman Bodies) in contrast to the control group. The histological structure of the injured liver did not fully recover in rats that had been castrated and fed 6 g/kg daily for 4 weeks. On the other hand, this treatment greatly reduced the adverse effects of castration, such as macrovesicular steatosis, Councilman Bodies, and mild ballooning degeneration. According to the current research, fresh M. longifolia extract may have some hepatoprotective benefits against castration
Immunohistochemical and Molecular Studies of p53 and KRAS Protein and Their Relations to Colorectal Carcinoma
No full textThe study inc1uded 50 tissue blocks embedded in paraffin wax (16 females and 34 males), obtained from a patients group with (CRC) colorectal cancer , as well as 35 Tissue blocks that were embedded in paraffin wax from norma1 co1on (ulcerative co1itis) as controls. A relatively few oncogenes and most prominently tumor-suppressing genes, Kirastien rat sarcoma virus (KRAS), and P53 genes have been mutated into a significant part of CRCs, and a broad collection of mutated genes has been defined in CRC subsets. Current findings showed very significant differences between patients and control subjects in the p53 positive rate (P<0.001). TP53 Pro/Pro genotype positivity was higher in the contro1 group I than in the patient group I and this was a significant difference (Pi<0.001) with an odd ratio of less than one. The genotype Pro/Pro was considered to be protective against colorectal carcinoma preventively fractured 0.767. The positive rate of p53 Arg/Arg genotype in patients was more frequent and statistically significant (P <0.01), because the odd ratio was more than one. The genotype Arg/Arg would be considered a colorectal carcinoma risk factor. We conclude that p53 over expression is used as an indicator of p53 mutation (as identified by immuno-historic chemistry) and KRAS protein expression was negatively impaired for all the patients in the current study
The Impact of Different Doses of Nickel Chloride on Some Biochemical and Histopathological Changes in the Liver of Rats
No full textThe present study investigated the effects of different doses of nickel chloride (NiCl₂) on biochemical markers and liver histology. Twenty-one young male rats, aged 3–4 weeks and weighing 150 - 200 g, were randomly assigned to three groups (N=7). The control group received only tap water, while the other two groups were exposed to nickel chloride at concentrations of 100 mg/kg and 150 mg/kg in their drinking water for six weeks. The results indicated no significant differences in biochemical parameters between the treated groups and the control subjects. Histological examination of liver tissues from rats exposed to nickel chloride (100 mg/kg and 150 mg/kg) revealed significant pathological changes. Observed abnormalities included cellular swelling, nuclear pyknosis, degeneration, necrosis, and blood vessel congestion, with higher doses leading to more pronounced damage. These findings suggest that nickel chloride poses a potential risk to liver health even at low concentrations and short exposure durations
The Essential Pheromonal Elements of the Mouse Preputial Gland in Castrated Treated Mice
No full textThe present study investigated the 1effects of both castration and manipulation of castrated1 subjects with 0.1 ml of 50µg/kg/day of testosterone at 3 weeks of age for 6 weeks on the pheromonal and histological structure of male mice preputial gland. The preputial gland results from the control intact group showed that six essential pheromonal compounds are present, whereas the glandular structure revealed well-developed acini with basement membrane and connective tissue around most of them. These findings also showed that castration has deleterious effects on the essential pheromonal compounds which are reduced to two compounds only (the α.-Farnesene and 1- Tetradecanol) as well as the histological sections from such subject appeared many atrophied vacuolated acini. On the other hand, treatment of castrated animals with such dose of testosterone did not show any marked effects on the essential pheromonal compounds but it was able to restore the activities of acinar development as showed by many hypertrophied alveoli
