130,423 research outputs found
Mutations in the Katnb1 gene cause left-right asymmetry and heart defects
No full textAbstract not availableMilena B. Furtado, D. Jo Merriner, Silke Berger, Danielle Rhodes, Duangporn Jamsai and Moira K. O, Brya
Ecological study of the tidal segment of the James River encompassing Hog Point : 1977 final technical report
No full textSection I: CONTENTS River Biota Studies at the VEPCO Surry Nuclear Power Station by R. A. Jordan, P. A. Goodwin, R. K. Carpenter, and C. E. Sutton Section Ila: Plant Entrainment of Ichthyoplankton at the VEPCO Nuclear Power Plant by J. V. Merriner, A. D. Estes, and R. K. Dias Section lIb: Thermal Plume Entrainment of Ichthyoplankton at VEPCO Nuclear Power Station by J. V. Merriner, A. D. Estes, and R. K. DiasVirginia Institute of Marine Scienc
Ecological study of the tidal segment of the James River encompassing Hog Point : 1975 final technical report
No full textVolume 1: Technical Reports Section 1: River Biota and Phytoplankton Entrainment Studies at the VEPCO Surry Nuclear Power Station Section 2: Zooplankton Entrainment at the Surry Nuclear Power Plant, James River, Virginia by G. C. Grant and B. B. Bryan Section 3: a. Plant Entrainment of Ichthyoplankton at the VEPCO Nuclear Power Station by J. V. Merriner and A. D. Estes b: Thermal Plume Entrainment of Ichthyoplankton at the· VEPCO Nuclear Power Station by J. V. Merriner and A. D. EstesVirginia Institute of Marine Scienc
KATNB1 in the human testis and its genetic variants in fertile and oligoasthenoteratozoospermic infertile men
No full textOligoasthenoteratozoospermia (OAT) is a phenotype frequently observed in infertile men, and is defined by low spermatozoa number, abnormal spermatozoa morphology and poor motility. We previously showed that a mutation in the Katnb1 gene in mice causes infertility because of OAT. The KATNB1 gene encodes an accessory subunit of the katanin microtubule-severing enzyme complex; this accessory subunit is thought to modulate microtubule-severing location and activity. We hypothesized that KATNB1 may play a role in human spermatogenesis and that genetic variants in KATNB1 could be associated with OAT in humans. Using immunostaining, we defined the localization of the KATNB1 protein in human testes. KATNB1 was present during spermatid development, and in particular localized to the microtubules of the manchette, a structure required for sperm head shaping. To assess a potential association between genetic variants in the KATNB1 gene and infertile men with OAT, we performed direct sequencing of genomic DNA samples from 100 OAT infertile and 100 proven fertile men. Thirty-seven KATNB1 variants were observed, five of which had not previously been described. Ten variants were present only in OAT men, however, statistical analysis did not reveal a significant association with fertility status. Our results suggest that variants in the KATNB1 gene are not commonly associated with OAT infertility in Australian men.L. O'Donnell, R. I. McLachlan, D. Jo Merriner, M. K. O'Bryan and D. Jamsa
Normal live birth after testicular sperm extraction and intracytoplasmic sperm injection in variant primary ciliary dyskinesia with completely immotile sperm and structurally abnormal sperm tails
No full textAbstract not availableRobert I. McLachlan, Tomomoto Ishikawa, Tiki Osianlis, Phil Robinson, Donna Jo Merriner, David Healy, David de Kretser and Moira K. O’Brya
GGN1 in the testis and ovary and its variance within the Australian fertile and infertile male population
No full textMouse gametogenetin (Ggn) is a testis-enriched gene that encodes multiple spliced transcripts giving rise to three predicted protein isoforms: GGN1, GGN2 and GGN3. Of these, GGN1 has been linked to germ cell development. Based on the spatial and temporal expression pattern of GGN1 during mouse spermatogenesis, it has been proposed as a candidate human infertility gene. Here, we report the localization of GGN1 in the human testis and ovary compared with the mouse orthologue. Within the testis, GGN1 was confined to pachytene spermatocytes and spermatids. During mid-prophase GGN1 redistributes from a solely cytoplasmic localization to both cytoplasmic and nuclear in late prophase spermatocytes and round spermatids, and is ultimately incorporated into the sperm tail. Within both mouse and human ovaries, GGN1 was localized within granulosa cells. Lower levels of expression were observed in mouse oocytes and the cumulus cells. Furthermore, to define the level of sequence variation in the fertile population and to assess the potential for an association with male infertility, we sequenced the coding region of human GGN in 100 idiopathic oligospermic infertile and 100 control men. Fifteen genetic variants were identified, of which 10 had not previously been reported. No significant associations with fertility status were observed, suggesting that variance in the GGN gene are not a common cause of oligospermic infertility in Australian men
MeSH term explosion and author rank improve expert recommendations
No full textInformation overload is an often-cited phenomenon that reduces the productivity, efficiency and efficacy of scientists. One challenge for scientists is to find appropriate collaborators in their research. The literature describes various solutions to the problem of expertise location, but most current approaches do not appear to be very suitable for expert recommendations in biomedical research. In this study, we present the development and initial evaluation of a vector space model-based algorithm to calculate researcher similarity using four inputs: 1) MeSH terms of publications; 2) MeSH terms and author rank; 3) exploded MeSH terms; and 4) exploded MeSH terms and author rank. We developed and evaluated the algorithm using a data set of 17,525 authors and their 22,542 papers. On average, our algorithms correctly predicted 2.5 of the top 5/10 coauthors of individual scientists. Exploded MeSH and author rank outperformed all other algorithms in accuracy, followed closely by MeSH and author rank. Our results show that the accuracy of MeSH term-based matching can be enhanced with other metadata such as author rank
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
"Closing the R&D Gap, Evaluating the Sources of R&D Spending"
No full textBoth spending and tax policies have been implemented in the United States with the goal of stimulating private sector research and development (R&D). Karier questions whether current R&D policy, especially the research and experimentation tax credit, can contribute to closing the gap between nondefense expenditures on R&D in the United States and such expenditures in other countries, such as Japan and Germany. He also explores possible changes to our current R&D policy to make it more effective.
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