17,398 research outputs found
Investigation of linkage of chromosome 8 to type 1 diabetes - Multipoint analysis and exclusion mapping of human chromosome 8 in 593 affected sib-pair families from the UK and US
No full textType 1 diabetes is a common multifactorial disease that is strongly clustered in families: the sibling risk-to-population prevalence ratio (lambda(s)) is 15 (6%/0.4%) (1). Two loci, IDDM1 in the major histocompatibility complex (MHC) on chromosome 6p21 and IDDM2 in the insulin gene (INS) region of chromosome 11p15.5 can account for similar to 50% of the observed familial clustering (1). Locus-specific lambda(s) values (1) in 356 U.K. affected sib-pair families (2) for IDDM1 and IDDM2 are 3.0 and 1.3, respectively, or 41 and 8%, assuming a multiplicative model (J.A.T., unpublished observations). The rapid reduction in risk from first- to second- to third-degree relatives of type 1 diabetic patients (3) and whole genome scanning of a spontaneous mouse model of autoimmune type 1 diabetes (4,5) suggest that other genes may account for the rest of the familial clustering (1,4,5). In humans, two genome-wide scans, both based on the analysis of affected sib pairs, have been published so far (6,7), with the larger scan evaluating 290 markers in 96 U.K. families (data were analyzed in 93 of these families, so henceforth, this scan will be referred to as the 93 U.K. family scan) (6). The main conclusions of these studies were that the presence elsewhere in the genome of a second gene with an effect similar to IDDM1 (lambda(s) = 3) is unlikely, and that other genes with lambda(s) < 3 are involved. Subsequently, additional evidence for linkage to some of these and other regions has been reported (8-10)
The Story of "Me" Contemporary American Autofiction
No full textCover -- Title Page -- Copyright Page -- Contents -- Acknowledgments -- Introduction -- 1. Masculinity, Whiteness, and Postmodern Self-Consciousness -- 2. Rage against the Dying of the Author -- 3. The New Journalism as the New Fiction -- 4. Trauma Autofiction, Dissociation, and the Authenticity of "Real" Experience -- 5. Memoir vs. Autofiction as the Story of Me vs. the Story of "Me" -- Coda -- Appendix -- Notes -- References -- IndexDescription based on publisher supplied metadata and other sources.Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, YYYY. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
DNA double strand breaks but not interstrand crosslinks prevent progress through meiosis in fully grown mouse oocytes
No full textThere is some interest in how mammalian oocytes respond to different types of DNA damage because of the increasing expectation of fertility preservation in women undergoing chemotherapy. Double strand breaks (DSBs) induced by ionizing radiation and agents such as neocarzinostatin (NCS), and interstrand crosslinks (ICLs) induced by alkylating agents such as mitomycin C (MMC), are toxic DNA lesions that need to be repaired for cell survival. Here we examined the effects of NCS and MMC treatment on oocytes collected from antral follicles in mice, because potentially such oocytes are readily collected from ovaries and do not need to be in vitro grown to achieve meiotic competency. We found that oocytes were sensitive to NCS, such that this ionizing radiation mimetic blocked meiosis I and caused fragmented DNA. In contrast, MMC had no impact on the completion of either meiosis I or II, even at extremely high doses. However, oocytes treated with MMC did show ?-H2AX foci and following their in vitro maturation and parthenogenetic activation the development of the subsequent embryos was severely compromised. Addition of MMC to 1-cell embryos caused a similarly poor level of development, demonstrating oocytes have eventual sensitivity to this ICL-inducing agent but this does not occur during their meiotic division. In oocytes, the association of Fanconi Anemia protein, FANCD2, with sites of ICL lesions was not apparent until entry into the embryonic cell cycle. In conclusion, meiotic maturation of oocytes is sensitive to DSBs but not ICLs. The ability of oocytes to tolerate severe ICL damage and yet complete meiosis, means that this type of DNA lesion goes unrepaired in oocytes but impacts on subsequent embryo quality
A male-female bias in type 1 diabetes and linkage to chromosome Xp in MHC HLA-DR3-positive patients
No full textIt is generally assumed that the male:female (M:F) ratio in patients with type 1 (insulin-dependent) diabetes mellitus (IDDM) is 1. A recent survey, however, revealed that high incidence countries (mainly European) have a high M:F ratio and low incidence ones (Asian and African) have a low M:F ratio(1) We have now analysed the M:F ratio according to genotype at the major locus, the major histocompatibility complex (MHC; IDDM1). There are two main IDDM1 susceptibility haplotypes, HLA-DR3 and -DR4, which are present in 95% of Caucasian cases(2-4). We report here that in medium/high incidence Caucasian populations from the United States of America, United Kingdom and Sardinia (1307 cases), the bias in male incidence is largely restricted to the DR3/X category of patients (X not equal DR4) with a M:F ratio of 1.7 (P = 9.3 x 10(-7)), compared with a ratio of 1.0 in the DR4/Y category (Y not equal DR3). This is additional evidence for significant heterogeneity between the aetiology of 'DR4-associated' and 'DR3-associated' diabetes(5-13). We analysed linkage of type 1 diabetes to chromosome X, and as expected, most of the linkage to Xp13-p11 was in the DR3/X affected sib-pair families (n=97; peak multipoint Mts at DXS1068 = 3.5, P = 2.7 x 10(-4); single point MLS = 4.5, P = 2.7 x 10(-5)). This is evidence for aetiological heterogeneity at the IDDM1/MHC locus and, therefore, in the search for non-MHC loci in type 1 diabetes, conditioning of linkage data by HLA type is advised
Saturation multipoint linkage mapping of chromosome 6q in type 1 diabetes
No full textLinkage analysis of type 1 diabetes sib pair families (n = 334) has suggested two separate regions of human chromosome 6q are linked to disease (designated IDDM5 and IDDM8), To test if these are false positive results, all available sib pair families (n = 429) were typed using a 92% informative map of chromosome 6q and multipoint analysis, The two regions still showed positive evidence of linkage, most notably the proterminal region, 6q27, corresponding to IDDM8 (MLS = 2.57, p = 0.0006; lambda(s) = 1.17). In addition, some evidence of transmission disequilibrium was seen with marker a046xa9 (IDDM5)
iii ORIENTATION DEPENDENCE OF DISLOCATION STRUCTURE EVOLUTION OF ALUMINUM ALLOYS IN 2-D AND 3-D ABSTRACT
No full textThe members of the Committee appointed to examine the thesis of COLIN CLARKE MERRIMAN find it satisfactory and recommend that it be accepted. Chair ii ACKNOWLEDGEMENTS I would like to express my deepest respect and gratitude to my advisor Dr. David P. Field for providing me the expert guidance, insight, vision, patience and tremendous help with the presented research. A special thanks Dr. Pankaj Trivedi and Scott Lindeman for their guidance, input, and assistance, without it I would have been lost while programming. I would also like to thank Dr. Hasso Weiland, Randy Burgess, and Julie Smith who are recognized for providing assistance in several areas. Lastly, Dr. Sergey Medyanik and Dr. David Bahr for their time and service as my master’s committee members
Remember Me A Novella about Finding Our Way to the Cross
No full textShades of Light.Cover -- Title Page -- Copyright -- Dedication Page -- Contents -- 1 The Word Became Flesh -- 2 The Gift of Myrrh -- 3 Taking the Cup -- 4 With a Kiss -- 5 Awakened -- 6 Accused -- 7 Bearing the Cross -- 8 Lament -- 9 Stripped -- 10 Pierced -- 11 It Is Finished -- 12 Into Your Hands -- 13 Buried -- 14 Risen -- Epilogue -- Journey to the Cross -- Acknowledgments -- Also Available -- Praise for Remember Me -- About the Author -- More Titles from InterVarsity PressShades of Light.Description based on publisher supplied metadata and other sources.Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, YYYY. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
"Test me and treat me" - attitudes to vitamin D deficiency and supplementation: a qualitative study
No full text© 2015 BMJ Open, "Test me and treat me"-attitudes to vitamin D deficiency and supplementation: a qualitative study. This manuscript version is made available under the Creative Commons Attribution Licens
‘At Me Too Someone is Looking’:Staging Surveillance in Irish Theatre
No full textThis chapter argues that Samuel Beckett’s plays function as a kind of fulcrum in a theatrical history of staging and thematising surveillance, and extends this history from Dion Boucicault and Augusta Gregory to Enda Walsh and David Lloyd. Surveillance agencies rely heavily on technology to gather information, but depend on human beings to store, order, and interpret it. ‘At Me Too Someone is Looking’ demonstrates that dramatic narratives exploit inconsistencies and injustices arising from slippages between data and its application
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