517 research outputs found
Optische Kohärenztomografie im Zeit- und Frequenzbereich
No full textIn dieser Arbeit wird auf den Einfluss nicht gaußförmiger Lichtquellen und dessen Potential eingegangen. Des Weiteren wird die Entstehung von Artefakten bei Messungen an Proben mit spektraler Reflektivität und dispersiven Proben erklärt und ein möglicher Umgang damit dargelegt. Weiterhin werden Vorteile der optischen Kohärenztomografie gegenüber der Konfokalmikroskopie erläutert. Während die Konfokalmikroskopie nur über die Intensität Positionen ermittelt, werden in der OCT die Intensität und die Phase ausgewertet. Zusätzlich tragen nur kohärente Teile des Lichts zu einem Signal bei. Es wird das Potential der optischen Kohärenztomografie gegenüber herkömmlichen Methoden deutlich und die Nutzung dieser modernen Technik praxisnah erklärt
The discovery of SycO reveals a new function for type three secretion effector chaperones
No full textThe Type Three Secretion (T3S) system is a device used by many Gram-negative pathogens that allows bacteria to deliver effector proteins straight into the eukaryotic cell cytosol. These effectors interfere with various signaling pathways to subvert the host cell functions. The secretion machinery of the T3S system consist of a basal body spanning the bacterial inner and outer membrane followed by a stiff hollow needle outside the bacterium. The fully assembled secretion apparatus constitute a continuous hollow conduit that connects the bacteria to the eukaryotic target cell. After cell contact, virulence proteins -called effectors- are injected directly into the cytosol of the host cell via the T3S apparatus. Several effectors of the T3S system require the assistance of specific cytosolic chaperones to be efficiently exported. There are three classes of T3S chaperones. Effector proteins are assisted by Class I chaperones. Although Class I chaperones are well characterized, their main function is still a matter of controversy. In this thesis, we demonstrate that orf155 encodes a specific chaperone for the effector YopO that we called SycO. We showed that SycO enhances YopO secretion in vitro and is required for translocation of YopO into infected cells. By pulldown assay we demonstrated that residues 20 to 77 of YopO are required and sufficient for SycO binding. Using crosslinking experiments and size exclusion chromatography analysis, we determined the stoichiometry of purified SycO and YopO-SycO complexes. SycO alone forms dimers in solution and the YopO-SycO complex has a 1:2 stoichiometry. These results suggested that SycO is a typical chaperone of the Class I. YopO is a serine/theronine kinase that interacts with Rho and Rac and disrupts the cytoskeleton of the target cells. YopO has been shown to localize at the cell plasma-membrane. By transfection of YopO-EGFP hybrid proteins into HEK293T cells, we demonstrated that the chaperone-binding domain (CBD) coincides with the membrane localization domain of YopO. Nevertheless, the CBD was not needed for the kinase activity of YopO. By ultracentrifugation, we also showed that the CBD causes YopO aggregation in the bacteria, when SycO does not cover it. Further, we show that the CBD of YopE and YopT also caused aggregation in the bacteria in the absence of SycE and SycT respectively. YopE, YopT and T3S effectors in other systems also act at the membrane of the eukaryotic host cell. We propose a new hypothesis concerning the role of T3S chaperones. The sub-cellular localization domain of effectors is aggregation-prone and creates the need for a chaperone inside bacteria. We propose that masking such aggregation-prone localization domains may be a general function for type III effector chaperones
Performance comparison of Intel Xeon Phi Knights Landing
No full textThe Intel Xeon Phi is a many-core processor with a theoretical peak performance of over 3 TFLOP/s of double precision. We contrast the performance of the second-generation Intel Xeon Phi, code-named Knights Landing (KNL), to the first-generation Intel Xeon Phi, codenamed Knights Corner (KNC), as well as to a node with two multi-core CPUs as baseline reference. The test code solves the classical elliptic test problem of the Poisson equation whose performance is prototypical for the computational kernel in many numerical methods for partial differential equations. The results show that the KNL can perform approximately four times faster than the KNC or than two CPUs, provided the problem fits into the 16 GB of on-chip MCDRAM memory of the KNL. The studies also confirm the nominal five times faster speed of the new high-performance MCDRAM memory in the KNL compared to the DDR4 memory of the node. We demonstrate the ease of porting code to the KNL by focusing on performance
that was achieved by only re-compiling hybrid MPI+OpenMP code with a KNL flag.These results were obtained as part of the REU Site: Interdisciplinary Program in High Performance Computing (hpcreu.umbc.edu) in the Department of Mathematics and Statistics at the University of Maryland, Baltimore County (UMBC) in Summer 2016. This program is funded by the National Science Foundation (NSF), the National Security Agency (NSA), and the Department of Defense (DOD), with additional support from UMBC, the Department of Mathematics and Statistics, the Center for Interdisciplinary Research and Consulting (CIRC), and the UMBC High Performance Computing Facility (HPCF). HPCF is supported by the U.S. National Science Foundation through the MRI program (grant nos. CNS–0821258 and CNS–1228778) and the SCREMS program (grant no. DMS–0821311), with additional substantial support from UMBC. Co-author Ishmail Jabbie
was supported, in part, by the UMBC National Security Agency (NSA) Scholars Program through a contract with the NSA. The authors thank both our team’s graduate assistant Jonathan Graf and faculty mentor Dr. Matthias K. Gobbert for their support throughout the program and beyond. Graduate assistant Jonathan Graf was supported by UMBC. The authors would like to thank the project client and collaborator Samuel Khuvis of ParaTools, Inc. for his support and connection with the Performance Research Laboratory, University of Oregon, that provided access to the KNL Hardware. This work used the Extreme Science and Engineering Discovery Environment (XSEDE), which is supported by National Science Foundation grant number ACI–1053575. We acknowledge the Texas Advanced Computing Center (TACC) at The University of Texas at Austin for providing HPC resources that have contributed to the research results reported within this paper.http://www.siam.org/Portals/0/Publications/SIURO/Volume%2010/Performance_comparison_Intel_Xeon_Phi_Knights_Landing.pdf?ver=2018-02-28-145959-11
Research in medical education - chances and challenges : international conference, 20th - 22nd May 2009, Heidelberg ; congress abstracts
No full textMainzer Maler - Maler in Mainz : Lebenswelten zwischen Stadt und Hof
No full textDiese Arbeit beschäftigt sich mit Mainzer Malern. Maler die in Mainz ansässig waren und hier das Bürgerrecht besaßen, sowie Maler, die für die Mainzer Erzbischöfe als Hofkünstler tätig waren. Große Namen wie Matthias Grünewald aber auch unbekannte Namen wie Conrad Kol oder Hans von Oppenheim.
Zeitlich bilden die Jahre 1462 – damals endete die Epoche der Stadtfreiheit – und 1631, damals wurde die Stadt im Dreißigjährigen Krieg von den Truppen Gustav Adolfs von Schweden erobert, die Eckpunkte der Untersuchung. Zu den zwischen den genannten Daten in Mainz ansässigen Malern und zu ihren Lebensbedingungen findet der gütige Leser ein wenig Text, dazu eine kleine Zusammenstellung der dem Autor bekannten Informationen zu einzelnen Malern, ein paar Diagramme, Tabellen und Karten und einen teilweise farbigen Abbildungsteil.
5 Bibliotheken, 15 Museen, 19 Archive, einige Semestertickets, 42 Bahntickets, 13.800 Blatt Papier, 16 Druckerpatronen, 4 Computer und mehr als 67 Liter Bier waren zu seiner Realisierung notwenig!The focus of this thesis are painters from Mainz, i.e. painters who were resident in and citizens of Mainz, as well as painters who worked for the archbishops of Mainz as court artists. They include well known names like Matthias Grünewald but also unknown names like Conrad Kol or Hans von Oppenheim.
Chronologically, the year 1462 - when the period of the independency of the city ended – and the year 1631, when the city was conquered during the 30-year war by the troops of Gustav Adolf of Sweden, are the cornerstones of the thesis. With regards to these painters, the reader finds some facts about their living conditions, information known to the author about individual painters, a few diagrams, tables and maps and a section with partially colored figures.
5 libraries, 15 museums, 19 archives, several semester tickets, 42 train tickets, 13.800 sheets of paper, 16 printer cartridges, 4 computers and more than 67 liters of beer were needed to make it possible
Mainzer Maler - Maler in Mainz : Lebenswelten zwischen Stadt und Hof
No full textDiese Arbeit beschäftigt sich mit Mainzer Malern. Maler die in Mainz ansässig waren und hier das Bürgerrecht besaßen, sowie Maler, die für die Mainzer Erzbischöfe als Hofkünstler tätig waren. Große Namen wie Matthias Grünewald aber auch unbekannte Namen wie Conrad Kol oder Hans von Oppenheim.
Zeitlich bilden die Jahre 1462 – damals endete die Epoche der Stadtfreiheit – und 1631, damals wurde die Stadt im Dreißigjährigen Krieg von den Truppen Gustav Adolfs von Schweden erobert, die Eckpunkte der Untersuchung. Zu den zwischen den genannten Daten in Mainz ansässigen Malern und zu ihren Lebensbedingungen findet der gütige Leser ein wenig Text, dazu eine kleine Zusammenstellung der dem Autor bekannten Informationen zu einzelnen Malern, ein paar Diagramme, Tabellen und Karten und einen teilweise farbigen Abbildungsteil.
5 Bibliotheken, 15 Museen, 19 Archive, einige Semestertickets, 42 Bahntickets, 13.800 Blatt Papier, 16 Druckerpatronen, 4 Computer und mehr als 67 Liter Bier waren zu seiner Realisierung notwenig!The focus of this thesis are painters from Mainz, i.e. painters who were resident in and citizens of Mainz, as well as painters who worked for the archbishops of Mainz as court artists. They include well known names like Matthias Grünewald but also unknown names like Conrad Kol or Hans von Oppenheim.
Chronologically, the year 1462 - when the period of the independency of the city ended – and the year 1631, when the city was conquered during the 30-year war by the troops of Gustav Adolf of Sweden, are the cornerstones of the thesis. With regards to these painters, the reader finds some facts about their living conditions, information known to the author about individual painters, a few diagrams, tables and maps and a section with partially colored figures.
5 libraries, 15 museums, 19 archives, several semester tickets, 42 train tickets, 13.800 sheets of paper, 16 printer cartridges, 4 computers and more than 67 liters of beer were needed to make it possible!1014 Seite
Countering the "Climate Cult" -- Cascading Frame Activation in Far-right Digital Networks (Supplementary Materials)
No full textThis is the supplementary material for the project "Countering the "Climate Cult" -- Framing Cascades in Far-right Digital Networks." We include our code in R Markdown format along with tables outlining the results of qualitative analyses and data cleaning. It also includes the Appendix, which features additional results and lists the dictionaries and intercoder reliability scores. The original dataset, needed to replicate the study and run the entire code, will be made available upon reasonable request to the corresponding author
Poetik und Politik der Lesbarkeit in der deutschen Literatur
No full textIn 1990, German literary critics agreed that the end of the Cold War should mark the end of politically committed post-war literature. The political caesura prompted a debate about the future of German literature during which the concept of 'readability' evolved as a contested issue. It was championed in particular by the author Matthias Politycki and the publishers Uwe Wittstock and Martin Hielscher. Ever since, 'readability' has remained a benchmark for authors and critics alike in the battle for value and success. The thesis will establish a theoretical basis for ‘readability’ that draws on narratology, the Aristotelian concept of ‘mimesis’, classical rhetoric, and the poetics of contemporary authors who explicitly engage with 'readability'. Discussion will centre on the novel since this genre has been the focus of debate ever since the novel gained prominence with the rise of the reading middle classes in the eighteenth century. An analysis of the historical role of 'readability' will demonstrate that the debate as it manifested itself around 1990 developed out of a specifically German tradition, in which authors and critics alike viewed it as potentially in conflict with true art. The thesis will demonstrate that 'readability' is key to understanding the debates about German literature in an era of globalisation when readers are more attracted to works by foreign authors than to works by German ones. It will examine how writers such as Helmut Krausser, Daniel Kehlmann, and Thomas Glavinic have exploited the opportunities of the changed parameters by writing and promoting 'readable' books. It will further explore to what extent 'readability' has opened up new avenues even for authors like Felicitas Hoppe and Ulrike Draesner, who distrust the quest for 'readability'. The thesis will conclude with a reflection on the prospects for 'readability' in the current literary landscape in Germany
Inca axeli Sousa & Seidel 2021, sp. nov.
No full textInca axeli sp. nov. urn:lsid:zoobank.org:act: C559A3D8-DD5D-4B6B-95A1-62276FAE6A79 Figs 1A–E, P, 2A–D, M, 3A, D, G–N, 4A Differential diagnosis Inca axeli sp. nov. is very similar to I. neglectus sp. nov. in that both species have clypeal horns in males with dorsolateral tooth long and acute (short and rounded in I. irroratus), posterior angles of pronotum acute (rounded in I. irroratus), medial area of elytra with large waxy maculae (absent in I. irroratus), and posterior tooth of protibia long and acute (short and rounded in I. irroratus). Inca axeli sp. nov. has the inner dorsal carina of clypeal horns abruptly interrupted at apex (gradually interrupted in I. neglectus sp. nov. and I. irroratus) and the outer distal process of parameres long and acuminate (long and rounded in I. neglectus sp. nov. and short and rounded in I. irroratus) (see Table 2). Etymology It is a pleasure for the last author to name the species after Axel González Gallardo in gratitude for the last years together and all his support. Material examined Holotype BRASIL – Santa Catarina • ♂; SC, Joinville; Dirings coll.; “HOLOTYPE, Inca axeli, Sousa & Seidel, 2019 ”; MZSP 22036. Paratypes (13 ♂♂ and 25 ♀♀) BRAZIL – No detailed localities • 1 ♂, 1 ♀; “Brasilien, Jäger coll., I. Burmeisteri, Brasil, Beske”; SMNS • 1 ♀; same collection data as for preceding; ZMH 833672 • 1 ♂; “ Brasil, Inca pulverulentus, Le Moult vend via Reinbek, Eing. Nr. 1, 1957”; ZMH 833671. – Amazonas • 1 ♀; “AM; Benjamin Constant; Rio Javary ”; Dec. 1961; Dirings coll.; MZSP 22053. – São Paulo • 1 ♀; “ Eugênio Lefreve, Pindamonhangaba, S.P.”; Mar. 1963; “EXp. Dep. Zoologia, INCA IRRORATUS, CHEVR., det. B.C. Ratcliffe 1983”; MZSP 22020 • 1 ♂; Est. Bio. Boraceia [Estação Biológica da Boracéia], Salesópolis; 1–4 Feb. 1973; Vanin coll.; “ INCA SP NOV., proche de irroratus [handwritten]; det. P. Bleuzen 1992”; MZSP 22007 • 1 ♀; São Paulo, (capital); Dirings coll.; Jan. 1960; MZSP 22054 • 1 ♀; S. Bocaína, Parq. criac. Trutas [parque de criação de trutas]; 1800 m a.s.l.; Mar. 1954; “Dalcy, R. Barros; Coll. IRSNB, ex coll. J. ROUCH, I.G.: 32.703”; RBINS. – Paraná • 1 ♂, 1 ♀; Piraquara, Manaciais da Serra; 3 Mar. 2012; CEMT • 1 ♂; Santo Antonio; 20 Mar. 1993; “Coll. P. BLEUZEN, Coll. IRSNB, eX coll. J. ROUCH, I.G.: 32.703, Photo N. Mal 2015”; RBINS. – Santa Catarina • 1 ♀; Joinville; Dirings coll.; MZSP 22051 • 1 ♂; Timbó; MZSP 22004 • 1 ♂; Timbó; Jun. 1969; Dirings coll.; MZSP 22035 • 1 ♂; Timbó; May 1956; Dirings coll.; MZSP 22038 • 1 ♀; Timbó; Feb. 1960; Dirings coll.; MZSP 22018 • 1 ♀; Timbó; Mar. 1960; Dirings coll.; MZSP 22019 • 7 ♀♀; Timbó; Mar. 1960; Dirings coll.; MZSP 22041–22047 • 3 ♀♀; Timbó; Jun. 1969; Dirings coll.; MZSP 22048–22050 • 1 ♀, 1 ♂; Rio Vermelho; Mar. 1949; Dirings coll.; MZSP 22039–22040 • 1 ♀; Rio Vermelho; Nov. 1963; Dirings coll.; MZSP 22052 • 1 ♂; CORUPA; Feb. 1952; “ Anton Maller; coleção CAMPOS SEABRA; Coll. IRSNB, eX coll. J. ROUCH, I.G.: 32.703”; RBINS • 1 ♀; CORUPA; “II”; “Anton Maller; coleção CAMPOS SEABRA; Coll. IRSNB, eX coll. J. ROUCH, I.G.: 32.703”; RBINS • 1 ♂; Campo Alegre; 23 Feb. 1997; “Coll. IRSNB, eX coll. J. ROUCH, I.G.: 32.703”; RBINS • 1 ♀; Jaragua do Sul; Feb. 1991; “Coll. Th. PORION; Coll. IRSNB, eX coll. J. ROUCH, I.G.: 32.703, Photo N. Mal 2015”; RBINS • 1 ♂; Hansa Humbolt; Mar. 1929; “ A. Maller; L. Burgeon, coll.et det., R.I.Sc. N.B.16.117”; RBINS • 1 ♂; “ Brésil, Sta Catarina ”, “ J.P.MARECHAL; Coll. IRSNB, eX coll. J. ROUCH, I.G.: 32.703”; Coll. Matthias Seidel 2019; MSPC • 1 ♀; Campo Alegre; 23 Feb. 1997; “Coll. IRSNB, eX coll. J. ROUCH, I.G.: 32.703”; Coll. Matthias Seidel 2019; MSPC. Paratypes deposited in CEMT, MZSP and UFRPE are labelled with “ PARATYPE; Inca axeli; Sousa & Seidel, 2019” and paratypes deposited in MSPC, RBINS, SMNS and ZMH are labelled with “ PARATYPE; Inca axeli sp. nov., M. Seidel & R. Sousa, 2019”. Description Holotype (male) BODY (Fig. 1A–C). Total length including clypeal horns 40 mm; width across humeri 17 mm. COLOUR. Reddish brown with dark green heterogeneous spots, dorsal surface with green metallic reflections; legs and meso- and metathoraX bright reddish brown (Fig. 1A–C, P). HEAD. Surface of frons with dark green and dark reddish-brown waxy secretion; clypeal horns with anterior area of inner dorsal carina truncate, abruptly interrupted and not reaching distal angles; dorsolateral tooth long and acute (Fig. 1P). THORAX. Lateral margin of pronotum strongly sinuous; lateromedial area with elongated and irregular fovea; longitudinal groove shallow; posterior angles acute (Fig. 1A–C). Anterior prosternal process acute, projected and densely setose in median area. Anterolateral area of scutellar shield punctate. Elytra with yellowish grey heterogeneous waxy maculae covering all surface and two large maculae in medial area (Fig. 1A). LEGS. Posterior tooth of protibia long and acute (Fig. 3A). Mesempodium with 2 setae. ABDOMEN. Fovea of sternite VII strongly marked. Disc of pygidium densely punctate, lateral area with well-defined punctures. TERMINALIA. Aedeagus: outer distal process of parameres long and acuminate (Fig. 1D–E). MORPHOLOGICAL VARIATIONS (males). Body length 30−45 mm, width 13−17 mm. Head: clypeal horns with or without green metallic reflection; brachycerous males with clypeal horns with dorsolateral tooth small and inner dorsal carina abruptly or gradually interrupted at apex. Thorax with anterior prosternal process rounded and small. Density of waxy maculae of elytra variable, maculae yellowish grey or golden yellow; the two large maculae in median area variable in shape and size. LEGS. Mesempodium with 2−4 setae. Female BODY (Fig. 2A–C). Length 32–42 mm; width across humeri 15–18 mm. Maculae of medial area of elytra usually larger than in males. Type locality Joinville, Santa Catarina State, Brazil. Distribution Brazil: São Paulo (Pindamonhangaba, Salesópolis, São Paulo) Paraná (Piraquara), Santa Catarina (Campo Alegre, Joinville, Corupá, Jaraguá do Sul, Timbó, Florianópolis, Bocaina do Sul) (Fig. 5). The female paratype from Benjamin Constant (Amazonas) is probably labelled incorrectly. Biology Two larvae of I. axeli sp. nov. were collected inside the base of a dead bromeliad (possibly Vriesea sp. or Aechmea sp.) in a fallen tree at Mananciais da Serra, Piraquara, Paraná State, Brazil (pers. com. Paschoal Grossi).Published as part of Sousa, Rafael & Seidel, Matthias, 2021, Review of the Inca irroratus species group with description of two new species of Inca LePeletier & Serville, 1828 (Coleoptera, Scarabaeidae, Cetoniinae), pp. 15-35 in European Journal of Taxonomy 748 (1) on pages 21-24, DOI: 10.5852/ejt.2021.748.1335, http://zenodo.org/record/473612
A Unified Model for Inter- and Intra-processor Concurrency
No full textAlthough concurrency is generally perceived to be a `hard' subject, it can in fact be very simple --- provided that the underlying model is simple. The occam-pi parallel processing language provides such a simple yet powerful concurrency model that is based on CSP and the pi-calculus. This thesis presents pony, the occam-pi Network Environment. occam-pi and pony provide a new, unified, concurrency model that bridges inter- and intra-processor concurrency. This enables the development of distributed applications in a transparent, dynamic and highly scalable way. The author specified the layout of the pony system as presented in this thesis, and carried out about 90% of the implementation. This thesis is structured into three main parts, as well as an introduction and an appendix. In the introduction, the need for a unified concurrency model is examined in detail. Thereupon, the pony environment is presented as a solution that provides such a unified model. The first part of this thesis is concerned with the usage of the pony environment for the development of distributed applications. It presents the interface between pony and the user-level code, as well as pony's configuration and a sample application. The second part presents the design and implementation of the pony environment. It explains the internal structure of pony, the implementation of pony's components and public processes, and the integration of pony in the KRoC compiler. The third part evaluates pony's performance and contains the final conclusions. It presents a number of performance tests and concludes with a discussion of the work presented in this thesis, along with an outline of possible future research
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