1,721,313 research outputs found
F-actin 결합 Cotl1 단백질의 미토콘드리아 다이나믹스 조절에 관여하는 역할
No full text비공개I. INTRODUCTION 1
II. MATERIALS AND METHODS 10
A. Annealing control primer (ACP)-based screening of differentially expressed genes 10
B. Plasmids, shRNA constructs and oligonucleotide sequence for siRNA 11
C. Cell culture and drugs treatment 12
D. Antibodies 12
E. Immunofluorescence 13
F. Confocal microscopy 13
G. Transmission electron microscopy 14
H. Primary cell culture 15
I. Quantification of mitochondrial length 16
J. Western blotting 16
K. Cell fractionation 17
L. Statistical analysis 17
III. RESULTS 18
A. Identification of differentially expressed genes among three types of HeLa cells with different mitochondrial morphology 18
B. Cellular localization of endogenous Cotl1 and overexpressed GFP-Cotl1 in HeLa, C2C12, and IMR90 cells 25
C. Changes in Cotl1 distribution in elongated mitochondria in shDrp1 cells 29
D. Changes in mitochondrial and F-actin morphologies in Cotl1-knock-down cells 32
E. Alterations in the expression of mitochondrial-shaping proteins in Cotl1-knockdown cells 37
F. Alterations in mitochondrial morphology and mitochondrial-shaping protein expression in primary cultured heart cells derived from Cotl1-knockout mouse 39
IV. DISCUSSION 42
V. CONCLUSION 46
REFERENCES 47
국문요약 55Maste
sj-docx-1-jvb-10.1177_0145482X241248461 - Supplemental material for Assessment of Level of Awareness and Barriers to Low Vision Rehabilitation Among Optometrists in Pakistan
No full textSupplemental material, sj-docx-1-jvb-10.1177_0145482X241248461 for Assessment of Level of Awareness and Barriers to Low Vision Rehabilitation Among Optometrists in Pakistan by Memoona Arshad, Aqsa Younas and Nida Amin in Journal of Visual Impairment & Blindness</p
sj-docx-2-jvb-10.1177_0145482X241248461 - Supplemental material for Assessment of Level of Awareness and Barriers to Low Vision Rehabilitation Among Optometrists in Pakistan
No full textSupplemental material, sj-docx-2-jvb-10.1177_0145482X241248461 for Assessment of Level of Awareness and Barriers to Low Vision Rehabilitation Among Optometrists in Pakistan by Memoona Arshad, Aqsa Younas and Nida Amin in Journal of Visual Impairment & Blindness</p
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Can coal replace other fossil fuels to fulfil the energy demand in Pakistan? An environmental impact analysis
No full textEnergy is considered as the backbone of an economy since all the production is dependent on energy consumption. Fossil fuels are currently the major energy source in most of the developing and developed countries. The use of fossil fuels as
energy source does not seem to support the concept of sustainability but in developing economies like Pakistan, the use of the indigenous fossil resources is vital to fulfill the energy demand of the country and to improve the socio-economic status
of the people. In this study, we have used environmental input–output (EIO) analysis for the estimation of direct and indirect CO2
emissions from fossil fuel consumption by the economy in year 2012. Keeping in view the huge coal reserves in Thar
Desert of Pakistan, we have developed a policy scenario in which coal is substituted by 100% for other fossil fuel types. Total CO2
emissions for this case are noted to be 16% higher than those estimated for 2012. CO2 emissions per capita come out to be
0.85 tons which is still less than that of India, China, USA, Middle East and Europe. This study recommends the use of indigenous coal as a short-term solution to the energy crisis in the country. Research and development activities should be escalated
for a gradual transition toward more sustainable energy systems
Interconnection of Cartoon Watching with Blood in Urine
No full textIf someone is interested in cartoon watching then there will be some chances to cause hematuria. The main motive of the study was to interconnect cartoon watching with blood in urine. Blood in urine causes hematuria. It causes serious diseases in human. It causes health problems. There were about 100 students involved in this research. All of them were the students of Bahauddin Zakariya University, Multan. They were between the age of 19-22. They shared their point of views about the affection and detest of cartoon watching
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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