1,720,965 research outputs found
Multiparametric Oled-Based Biosensor for Rapid Dengue Serotype Recognition With a New Point-Of-Care Serological Test
No full textA deep-blue OLED-based biochip for protein microarray fluorescence detection
No full textIntegrated biochips exploit a multi-disciplinary approach to produce portable point-of-care medical diagnostic systems that uncouple diagnosis from centralized laboratories. These portable devices are cost effective and have several advantages including broader accessibility to health care worldwide. Fluorescence detection of a disease-specific probe excited by an optical source is one of the most diffused methods for quantitative analysis on biochips. Here we designed and characterized a miniaturized biochip based on a novel deep-blue organic light-emitting diode. The molecular design of the diode was optimized to excite a fluorophore-conjugated antibody and tested on a protein microarray configuration with good sensitivity and specificity. These findings will be instrumental for the development of next generation point-of-care biochips. (C) 2013 Elsevier B.V. All rights reserved
Optimization of an OLED-based immunosensor for the detection of tetrodotoxin in mussels
No full textAlien species have colonized new aquatic ecosystems due to multifactorial effects, among which climate change or the increasing marine traffic, can be mentioned. The occurrence of contamination due to tetrodotoxin (TTX) is now observed in the Mediterranean Sea and in bivalves, whereas TTX was classically contaminating pufferfish in the Pacific Ocean. In this paper, we present the optimization of an Organic Light Emitting Diode (OLED) based immunosensor to detect tetrodotoxin in spiked samples of mussels. An ELISA test was preliminary optimized to set the concentrations of all biological elements required to develop the OLED-immunoaffinity-based biosensor and to mutually validate the two detection systems presently optimized. The threshold concentration of 44 ng g−1 set by EFSA for TTX in seafood products was used to distinguish the negative mussel samples from the positive ones. A streamlined extraction protocol was adopted after its optimization to fulfil the need of the assay (European Food Safety Authority, 2017)
A deep-blue OLED-based biochip for protein microarray fluorescence detection.
No full textIntegrated biochips exploit a multi-disciplinary approach to produce portable point-of-care medical diagnostic systems that uncouple diagnosis from centralized laboratories. These portable devices are cost effective and have several advantages including broader accessibility to health care worldwide. Fluorescence detection of a disease-specific probe excited by an optical source is one of the most diffused methods for quantitative analysis on biochips. Here we designed and characterized a miniaturized biochip based on a novel deep-blue organic light-emitting diode. The molecular design of the diode was optimized to excite a fluorophore-conjugated antibody and tested on a protein microarray configuration with good sensitivity and specificity. These findings will be instrumental for the development of next generation point-of-care biochips
Development of a point of care (POC) test as an immunobiosensor for okadaic acid detection in mussels
Full text linkThe increase in frequency and intensity of potential risks concerning the exposure of seafood to diarrhetic shellfish poisoning (DSP) toxins, even in areas not traditionally accomplished, raises the number of monitoring analyses for the detection of this class of toxins to guarantee the food safety of the products. In addition to the analysis performed with liquid chromatography-tandem mass spectrometry (LC-MS/MS), reference method in EU, semiquantitative rapid tests for preliminary screening and rapid monitoring plan are applied. Different kits for the detection of DTX family toxins are available on the market, but they require specialised labs and the cost of the analysis per sample is quite high. Based on the current situation, developing an easy-touse and cheap test for measuring okadaic acid, as the main responsible for DSP poisoning, for the implementation of point-of-care (POC) systems is desirable. An immunobiosensor test, based on immunoaffinity reaction utilizing commercially available monoclonal antibodies, has been optimized and validated for the detection of okadaic acid in mussel (Mytilus galloprovincialis) extracts, with a limit of detection of 60 mu g/kg, a sensitivity of 0.68 n degrees of counts/ ug/Kg and a screening test range between of 60-350 ug/kg. The proposed POC immunoassay provides results comparable (r = 0.981) to the ones obtained by other semiquantitative rapide tests, like enzymatic assay applied for routine monitoring plans for the detection of the dinophysistoxins (DTXs) family. The goal of the presented test is the reduction of steps for the toxin extraction and a relevant reduction of the time for the analysis and the provision of a cheap POC analysis system
Campylobacter detection in poultry meat by using an organic light-emitting diode based biochip
No full text
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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