1,723,274 research outputs found
Eretmocerus iulii Laudonia et Melone sp. n.: parasitoid associated with Aleurocanthus spiniferus
Full text linkA meticulous study has led to description and identification of a novel member of the aphelinid genus Eretmocerus as a species
associated with nymphs of the orange spiny whitefly, Aleurocanthus spiniferus (Quaintance) (Hemiptera Aleyrodidae). The detailed
morphological analysis of the parasitoid has revealed distinctive characteristics that differentiate it from known species within the
genus. Molecular analysis has been conducted to provide additional evidence to the morphological analyses. Molecular data obtained
from DNA sequencing of the mitochondrial COI and 28S ribosomal genes has been compared with sequences from existing
databases, revealing a genetic divergence from other available Eretmocerus species and genetically defining the studied entity. The
integrated methodology employed in this study enabled the delineation of a new species within the Eretmocerus serius Silvestri
group, Eretmocerus iulii Laudonia et Melone sp. n. (Hymenoptera Aphelinidae)
GLT-1 up-regulation impairs prepulse inhibition of the startle reflex in adult rats
No full textMELONE M. and BELLESI M: co-first autho
Increased expression of the astrocytic glutamate transporter GLT-1 in the prefrontal cortex of schizophrenics
No full textMELONE M. and MATUTE C.: co-first author
ABSTRACT
To verify whether altered glial glutamate uptake contributes to the
reduced efficacy of glutamatergic transmission reported in the prefrontal cortex of
schizophrenics, we studied the expression of GLT-1, the transporter responsible for most
glutamate transport, in autoptic samples of prefrontal cortex using real time quantitative
RT-PCR, immunocytochemistry, and functional assays. GLT-1 mRNA levels in
medication-free patients were 2.5-fold higher than in controls, whereas they were
normal or reduced in patients treated with antipsychotics. We also observed a 4-fold
increase in L-[3H]-Glu uptake in Xenopus oocytes injected with mRNA from the prefrontal
cortex of a medication-free schizophrenic and a 2-fold increase in GLT-1 protein in
the same cortical area of another medication-free patient. Results suggest that GLT-1
mRNA, protein and function are increased in prefrontal cortex of schizophrenics
Solatisonax bannocki Melone & Taviani 1980
No full textSolatisonax bannocki (Melone & Taviani, 1980) Fig. 16 l–n Architectonica bannocki Melone & Taviani, 1980 (p. 97, figs. 1–2). Solatisonax bannocki (Melone & Taviani, 1980) —Melone & Taviani 1984 (p. 157, figs. 12–1); Repetto et al. 2005 (p. 224, bottom right fig.). Diagnostic characters. Low-spired, almost discoidal shell; weak subsutural shoulder giving the spire a somewhat step-like profile; markedly prosocline rhomboidal aperture; wide and very deep umbilicus bordered by a row of knobs; robust peripheral keel; numerous fine spiral threads cut into minute granules by dense radial striae. Protoconch: heterostrophic; about 2 whorls, the first deeply immersed; diameter about 800 µm; surface smooth; transition to the teleoconch marked by a thin, everted lip. Occurrence. Box-corer samples BC11 (2 specimens), BC67 (1), BC68 (1), BC70 (4), BC71 (1), BC72 (11). Maximum diameter: 10 mm. Distribution and habitat. Solatisonax bannocki is distributed in the western Mediterranean basin and in the nearby Atlantic, as far south as Cape Verde; it is a bathyal species dwelling on mud and probably associated with deep-water corals (Melone & Taviani 1980, 1984). The present occurrence could be the easternmost record of the species in the Mediterranean. Fossil record. Upper and Middle Pleistocene of Calabria and Sicily Channel (Melone & Taviani 1984).Published as part of Negri, Mauro Pietro & Corselli, Cesare, 2016, Bathyal Mollusca from the cold-water coral biotope of Santa Maria di Leuca (Apulian margin, southern Italy), pp. 1-97 in Zootaxa 4186 (1) on page 73, DOI: 10.11646/zootaxa.4186.1.1, http://zenodo.org/record/16528
Listeria, il melone diventa vettore
No full textDescrizione di una epidemia di Listeria moocytogenes da melone negli Stati Unit
Improving melon (Cucumis melon L.) productivity and quality through the use of molecular markers for biotic stress tolerance.
No full textembargoed_20271205Questa tesi propone una strategia avanzata per migliorare la produttività e la qualità del melone utilizzando marcatori molecolari legati alla tolleranza all'oidio, un patogeno che compromette resa e conservabilità del raccolto. Il lavoro si è concentrato sull'identificazione di marcatori genetici associati alla tolleranza all'oidio in varietà di melone. Tramite il sequenziamento Next-Generation Sequencing (NGS) è stato individuato il marcatore SNP421, correlato a una maggiore tolleranza alla malattia. Questo SNP è stato validato attraverso diverse tecniche di validazione, tra cui l’analisi High-Resolution Melting (HRM), il sequenziamento Sanger e saggi di discriminazione allelica rhAmp, dimostrandosi efficace per una selezione assistita più efficiente rispetto ai metodi fenotipici tradizionali. L'adozione di SNP421 nei programmi di breeding consente di ottenere piante meno suscettibili all'oidio, riducendo il bisogno di trattamenti chimici e migliorando shelf- life e qualità del frutto. SNP421 rappresenta dunque uno strumento chiave per una produzione di melone più sostenibile e competitiva, che risponde alle sfide del mercato e ai requisiti di sicurezza alimentare.This thesis proposes an advanced strategy to improve the productivity and quality of melon using molecular markers associated with powdery mildew tolerance, a pathogen that compromises yield and crop shelf life. The work focused on identifying genetic markers associated with powdery mildew tolerance in melon varieties. Through Next- Generation Sequencing (NGS), the SNP421 marker was identified, which is linked to increased disease tolerance. This SNP was validated using several validation techniques, including High-Resolution Melting (HRM) analysis, Sanger sequencing, and rhAmp allele discrimination assays, proving effective for a more efficient marker-assisted selection compared to traditional phenotypic methods. The adoption of SNP421 in breeding programs enables the development of plants less susceptible to powdery mildew, reducing the need for chemical treatments and enhancing fruit shelf life and quality. Therefore, SNP421 represents a key tool for a more sustainable and competitive melon production that meets market challenges and food safety requirements
Spongelike Functional Materials fromTEMPO-Oxidized Cellulose Nanofibers
No full textThe cleavage of the hierarchical structure of plant cellulose provides nanocellulose (NC), an attractive renewable nanomaterial for advanced applications. The presence of carboxylic acid moieties renders 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-oxidized cellulose nanofibers (TOCNF) particularly appealing for the synthesis of cellulose-based polymers with suitable cross-linkers. This chapter aims to provide an overview on the synthesis of bPEI-TOCNF sponges and their possible application fields. The high specific content of amino groups in bPEI-TOCNF makes them very suitable for environmental applications. The nanostructuring of cellulose nanofibers can also represent a useful synthetic tool for the preparation of different families of organic and inorganic aerogels. The cross-linking of TOCNF, in the presence of polyamines, provides a simple and cheap thermal procedure for the production of nanostructured aerogels from polysaccharide renewable sources. The ecofriendly nature of the engineered nanostructured materials opens the route for the development of sustainable and ecosafe nanoremediation, overcoming the issues related to the use of nanosized technologies
Cyclodextrins functionalized with nitroxyl persistent radicals: synthesis, magnetic behaviour and applications
No full textMagnetic behavior of cyclodextrin-based nanosponges bearing nitroxyl persistent radicals
No full textParamagnetic organic materials are attracting an increasing interest due to their potential use in nanomedicine. In particular, it has been reported that polymeric matrices containing TEMPO radical moieties are capable to combine drug delivery properties with intracellular scavenging action of reactive oxygen species.
Paramagnetic cyclodextrin nanosponges (pmCDNS) are a new class of nanodevices with potential application in nanomedicine, due to the following properties: a) adsorption and release capability of bioactive molecules; b) detectability by EPR and MRI spectroscopies during in vitro and in vivo experiments thanks to the presence of spin labels. Moreover, they may open the way to the synthesis of metal-free organic magnets.
pmCDNS were prepared by reaction in anhydrous DMSO of a mono-functionalized β-cyclodextrin (βCD), bearing a TEMPO radical moiety, with suitable cross-linkers (CL) like EDTA or pyromellitic dianhydride (PMA), with variable βCD/CL molar ratio. An alternative approach consisted into the one-pot reaction between pristine βCD, CL and a phthalic anhydride derivative bearing a TEMPO radical unit. The paramagnetic behavior of the obtained materials was investigated by EPR spectroscopy and SQUID magnetometry. Magnetic measurements, corrected for the diamagnetic contribution of the nanosponge matrix, displayed a Curie-Weiss behaviour with a negative paramagnetic Curie temperature (p = -4.3 K), suggesting that antiferromagnetic interactions were predominant in this system. The isothermal magnetisation at 2K was well fitted by the Brillouin function with a single spin moment (J=1⁄2), as expected by the TEMPO radical. A quantitative evaluation of the magnetic data suggested that around 50 mg of TEMPO radicals were present per gram of material
GLT-1 promoter activity in astrocytes and neurons of mouse hippocampus and somatic sensory cortex
Full text linkGLT-1 eGFP BAC reporter transgenic adult mice were used to detect GLT-1 gene expression in individual cells of CA1, CA3 and SI, and eGFP fl uorescence was measured to analyze quantitatively GLT-1 promoter activity in different cells of neocortex and hippocampus. Virtually all GFAP+ astrocytes were eGFP+; we also found that about 80% of neurons in CA3 pyramidal layer, 10-70% of neurons in I-VI layers of SI and rare neurons in all strata of CA1 and in strata oriens and radiatum of CA3 were eGFP+. Analysis of eGFP intensity showed that astrocytes had a higher GLT-1 promoter activity in SI than in CA1 and CA3, and that neurons had the highest levels of GLT-1 promoter activity in CA3 stratum pyramidale and in layer VI of SI. Finally, we observed that the intensity of GLT-1 promoter activity in neurons is 1-20% of that measured in astrocytes. These results showed that in the hippocampus and neocortex GLT-1 promoter activity is observed in astrocytes and neurons, detailed the distribution of GLT-1 expressing neurons, and indicated that GLT-1 promoter activity in both astrocytes and neurons varies in different brain regions. © 2010 de Vivo, Melone, Rothstein and Conti
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