1,720,955 research outputs found
High throughput sequencing reveals the Upregulation of PNMA3 and RASSF2 associated with chemoradiotherapy resistance in cervical cancer patients
No full textCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorCervical cancer (CC) affects women in global scale and many factors are responsible for the high mortality rate related to this cancer, such as chemoradiotherapy resistance including the cisplatin resistance (CPR) and late diagnosis. Between the hallmarks of cancer is the inhibition of apoptotic pathways, a modulation weakly knows considering both the chemoradiotherapy resistance and CC. Thus, the objective of this study was to identify differentially expressed genes (DEGs) related to apoptosis between Responder (R) and Non-responder (NR) patients to the chemoradiotherapy, as well as the number of immune cells in the tumor microenvironment (TME). For this purpose, 21 R and 10 NR women were included in this study and your cervical cancer non-stem-like cells (CCNSCs) were selected in Fluorescence-activated cell sorting (FACS). Then, the RNA-Seq was performed and all sequences were evaluated according to quality, and those with Phread 1 or < -1 and pajd value < 0.05 were found, 117 of them were linked to apoptosis and only 8 were relevant according to statistical and decision tree analyses. Between the relevant DEGs, the PNMA3 and RASSF2 were upregulated in NR patients and downregulated in R considering all analyses performed. Furthermore, a high number of intratumoral TILs and TATES occurred in the R group, while the NR had a high number of stromal TILs. Therefore, the genes PNMA3 and RASSF2 upregulated in NR patients were considered as potential biomarkers related to apoptosis and chemoradiotherapy resistance, as well as a larger nº of intratumoral TILs and TATES were associated to the positive chemoradiotherapy responses in CC. Nonetheless, more studies still need to be done to confirm the likely interactions among PNMA3, RASSF2, apoptosis and chemoradiotherapy resistance in the CC.Dissertação (Mestrado)O câncer de colo uterino (CCU) afeta mulheres no mundo todo e os índices de mortalidade devido a esta patologia são próximos de 50%, o que é devido principalmente ao seu diagnóstico tardio e à resistência a tratamentos quimioradioterápicos como à cisplatina (RCP). Hallmarks do câncer como a inibição da apoptose são associadas a esta resistência, mas os fatores envolvidos nestas vias de morte celular no CCU ainda são pouco conhecidos. Assim, o objetivo deste trabalho foi identificar genes diferencialmente expressos (DEGs) relacionados à apoptose entre pacientes Respondedoras (R) e Não-respondedoras (NR) à quimioradioterapia, bem como o número de células imune no microambiente tumoral destas mulheres. Com esta finalidade, 21 pacientes R e 10 NR foram incluídas no estudo e suas células não-tronco tumorais (CNTTs) foram selecionadas por citometria de fluxo e submetidas a sequenciamentos de RNA. Todas sequencias obtidas com baixa qualidade e adaptadores foram removidos, enquanto aquelas selecionadas foram mapeadas unicamente a loci específicos do genoma humano e quantificadas quanto à sua expressão. Por fim, análises de expressão diferencial gênica, de ontologia gênica (GO), estatísticas e árvores de decisão foram realizadas para a obtenção de potenciais biomarcadores da resistência quimioradioterápica. Paralelamente foi feita a avaliação morfométrica do nº de linfócitos infiltrantes tumorais (TILs) e eosinófilos associados ao tecido tumoral (TATES) de 23 pacientes que atenderam aos critérios de inclusão. Ao todo 2519
DEGs com log2FC > 1 ou < -1 e valor de p.ajustado < 0.05 foram considerados, sendo 117 deles associados a apoptose e somente 8 relevantes conforme análises de GO, estatística e de árvore de decisão. Destes 8 DEGs, PNMA3 e RASSF2 foram encontrados superexpressos em pacientes NR e com baixa expressão em R, conforme valores de log2FC, z-score e a razão entre médias normalizadas de expressão entre os grupos R e NR. Além disso, o número de TILs intratumorais e TATES foram superiores em pacientes R enquanto no grupo NR houve um maior número no ambiente estromal. Portanto, os genes PNMA3 e RASSF2 foram definidos como potenciais biomarcadores associados a apoptose e à resistência quimioradioterápica, visto a sua predominância em pacientes NR e os inúmeros trabalhos associando as famílias destes genes com a regulação da apoptose. Adicionalmente, um maior nº de TILs e TATES no microambiente intratumoral foram associados com respostas quimioradioterápicas favoráveis no CCU, mas mais estudos são necessários para caracterizar tanto estes DEGs quanto o perfil destas células imune neste câncer
Influência do polimorfismo -196/-174 del do receptor toll-like 2 (tlr2) na susceptibilidade ao câncer de próstata
Full text linkThe Toll-like family (TLR) represents the main group of receptors related to innate immune system that respond against antigenic factors, including to Damage-Associated Molecular Patterns (DAMPs), which are produced by cancerous cells. Considering the Prostate Cancer (PCa), the genetic regulation, the cell signaling, the secreted cytokines and the recruited immune cells related to TLR2 activation, still need to be elucidated. This work aimed to characterize the -196/-174del polymorphism of TLR2 in PCa susceptibility in Brazilian men. Through allele-specific PCR, 23 patients with PCa and 23 with Benign prostatic hyperplasia (BPH) were genotyped. Men were considered as Ins/Ins (Insertion/Insertion),when both alleles were present(288bp), Ins/Del as heterozygous and Del/Del (Deletion/Deletion) with both 266bp alleles. There were no statistically significant difference of age and PSA values between the studied groups (p= 0.12 and p= 0.17, respectively). The genotypic frequency was in Hardy-Weinberg Equilibrium (HWE) (p=0.90 for PCa and 0.43 for BPH). Furthermore, the genotypes did not differentiate PCa and BPH patients; however, there was a higher prevalence of deleterious allele in men affected by the disease. A greater number of individuals need to be evaluated, as well as analyzes of the transcripts number of TLR2 gene must be conducted to define the impact of this mutation in prostatic lesions.CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoTrabalho de Conclusão de Curso (Graduação)A família de receptores Toll-like (TLR) representa o principal grupo de receptores responsável pelas respostas do sistema imune inato a fatores antigênicos, incluindo aos Padrões Moleculares Associados a Danos celulares (DAMPs), os quais são produzidos por células cancerígenas. Considerando o Câncer de Próstata (CaP), a regulação gênica, a sinalização celular, a consequente secreção de citocinas e o recrutamento de células imunes relacionadas à ativação de TLR2 ainda necessitam ser descritos. Este trabalho objetivou verificar a influência do polimorfismo -196/-174del deste receptor na susceptibilidade ao CaP em indivíduos brasileiros. Por meio de PCR alelo-específica, 23 pacientes com CaP e 23 com hiperplasia prostática benigna (HPB) foram genotipados. Estes foram caracterizados em Ins/Ins (Inserção/Inserção), quando ambos os seus alelos continham 288 pb, Ins/Del (Inserção/Deleção) como heterozigotos e Del/Del (Deleção/Deleção) quando continham alelos com 266pb. Não foi verificada diferença estatisticamente significante entre as idades e os valores de PSA (Antígeno Prostático Específico) entre os grupos de estudo (p = 0,12 e p = 0,17, respectivamente). A frequência genotípica se mostrou em equilíbrio de Hardy-Weinberg (p=0.90 para CaP e 0.43 para HPB). Além disso, os genótipos não diferenciaram os pacientes CaPde HPB, contudo, houve uma maior prevalência do alelo deletério em homens acometidos pela doença. Um maior número de indivíduos necessita ser avaliado, bem como análises do número de transcritos do gene TLR2 devem ser conduzidas para, assim, definir o impacto dessa mutação nas lesões prostáticas
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
- …
