1,721,412 research outputs found
Microbiological diagnosis of invasive aspergillosis
No full textItem does not contain fulltextKUN, 01 februari 1997Promotor : Hoogkamp-Korstanje, J.A.A. Co-promotores : Meis, J.F.G.M., Pauw, B.E. d
Molecular epidemiology, environmental dispersion and antifungal susceptibility of Cryptococcus grubii and C. gattii prevalent in India
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100890.pdf (Publisher’s version ) (Open Access)Radboud Universiteit Nijmegen, 18 juni 2012Promotor : Mouton †, J.W. Co-promotores : Meis, J.F.G.M., Klaassen, C.H.W
Tackling histoplasmosis infection in Latin America: From diagnostic strategy to public health solutions
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321028.pdf (Publisher’s version ) (Open Access)Radboud University, 02 september 2025Promotor : Verweij, P.E. Co-promotores : Meis, J.F.G.M., Chiller, T., Gomez, B.157 p
Cryptococcosis in Southern-Brazil: Molecular, antifungal and in vitro cytokine induction studies
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201859.pdf (Publisher’s version ) (Open Access)Radboud University, 11 april 2019Promotor : Verweij, P.E. Co-promotores : Meis, J.F.G.M., Hagen, F
Molecular diagnosis and characterisation of Yersinia enterocolitica infection
No full textItem does not contain fulltextKUN, 07 december 1998Promotor : Hoogkamp-Korstanje, J.A.A. Co-promotores : Melchers, W.J.G., Meis, J.F.G.M
Cryptococcus neoformans complex: molecular, clinical and in-vitro studies.
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128930.pdf (Publisher’s version ) (Open Access)Radboud Universiteit Nijmegen, 27 augustus 2014Promotores : Voss, A., Verweij, P.E. Co-promotores : Meis, J.F.G.M., Hagen, F
Exact Molecular Typing of Aspergillus fumigatus. Methods and Applications.
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65557_exacmotyo.pdf (Publisher’s version ) (Open Access)
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70295.pdf (Publisher’s version ) (Open Access)Aspergillus species are widely distributed fungi that release large amounts of airborne conidia that are dispersed in the environment. Aspergillus fumigatus is the species most frequently isolated from human infections. In this thesis a novel assay for fingerprinting A. fumigatus is described and evaluated. This assay demonstrate several characteristics important for a good typing assay. Of major importance was the ability to discriminate between unrelated isolates. Typing results from epidemiological related collections showed identical genotypes and the results are in concordance with other high discriminatory typing techniques. Other advantages include the requirement of low amounts of DNA, and the automated sizing of amplification products result in the relatively easy interpretation of the data. The STRAf assay is also rapid and has a high throughput by multiplexing. The assay is robust, which makes it possible to successfully use the assay in other settings. Together with the use of allelic ladders it is even possible to exchange fingerprint typing data between laboratories, provided that they have access to high resolution electrophoresis platforms. In CF patients different colonization patterns were found with the STRAf assay. In IA patients the uniqueness of clinical isolate and the dissemination route are evaluated. With the option to genotype A. fumigatus directly in FFPE-tissue samples and serum samples, it is possible to retrospectively examine dissemination routes in IA patients without A. fumigatus cultures. In outbreak settings, where besides clinical isolates also environmental isolates were analyzed, variations in a single loci between epidemiological related isolates were found. The knowledge of the rate of change of the individual markers of the STRAf assay, may provide good guidelines for the interpretation of different STRAf typing patterns. The high throughput of samples, the low costs, the robustness and the interlaboratory reproducibility together with the high discrimination power makes this assay suitable for fingerprinting large amount of isolates and exchange of results between labs worldwideRU Radboud Universiteit Nijmegen, 25 november 2008Promotor : Voss, A. Co-promotores : Klaassen, C.H.W., Meis, J.F.G.M.205 p
Exoerythrocytic development of a rodent malaria parasite: an ultrastructural study on Plasmodium berghei in rat liver
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mmubn000001_027884929.pdf (Publisher’s version ) (Open Access)Promotores : J. Meuwissen en S. Wendelaar Bonga167 p
Antifungal susceptibility testing and drug interaction modeling in moulds
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19230_antisutea.pdf (Publisher’s version ) (Open Access)In this thesis two parameters of in vitro antifungal susceptibility testing of filamentous fungi, the medium and the quantification of fungal growth, were investigated and different drug interaction models were used for analyzing the in vitro combination of various antifungal drugs. A microbroth kinetic system was developed in order to analyze the growth curves of three species of filamentous fungi in five different nutrient media. Two colorimetric methods based on the reduction of the tetrazolium salts, MTT and XTT, was evaluated for the MIC determination of different antifungal drugs against different species of filamentous fungi. High levels of agreement were found between the colorimetric medthods and the NCCLS method for all drugs and species tested. The in vitro combination of terbinafine with three azoles, namely itraconazole, voriconazole and miconazole was tested against clinical S. prolificans isolates using the spectrophotometric method, the colorimetric MTT method and a modified MTT method. In addition, the results were analyzed with parametric and non-parametric approaches of the Loewe additivity and Bliss independence zero interaction theories. Strong synergy was found between terbinafine and the three azoles with miconazole and voriconazole showing the strongest synergy based on Loewe additivity and Bliss independece theory, respectively. Less variable results were obtained with the modified MTT method. Despite its simplicity, results of the FIC index model depended on the choice of MIC endpoints and interpretation endpoints. Furthermore it lacked of good summary. Although the model described by Prichard et al. resulted in a concentration dependent mosaic of interactions, replicates were required for statistical evaluation of the results, good summary was absent and the results were dependent on the concentration range used. The fully parametric model described by Greco et al., although it did not describe precisely the response surface of antifungal combinations, was able to distinguish synergistic and antagonistic interactions and summarized the interaction with a non-unit concentration independent interaction parameter including statistically significance levels without requiring replicates130 p
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