1,721,302 research outputs found
Development and validation of short amplicon-length PCR assays for the detection of feline species in processed foods / Md. Al Amin
Full text linkFood falsification is a common concern, but has been mutually practiced in the meat industry, especially for processing food products, for realizing an extra profit. The
everyday happenings such horse, porcine, rat and dog meats forgeries in various foods have made consumers increasingly worried to safeguard their religious faith, health, money and wildlife in natural habitats. The consumers of the Halal food market have reached to 1.8 billion and turnover has crossed US Dollar 700 billion in 2012 and it has been projected to reach at US$ 1.6 trillion by the 2030. Since the market is quite large and opportunities in halal food business are huge, it has been targeted for adulteration for
a long time.
Consumption or mixing of feline ingredients in halal and kosher foods is forbidden and various diseases such as SARS, anthrax and hepatitis could be transmitted through feline meats. However, since feline species are abundant across the world without market price and their meats are consumed in exotic foods, the chances of their adulteration in common meats are very high. For meat specification, DNA-based techniques are preferred over protein and lipid-based molecular identification schemes since DNA biomarkers, especially the short-length one, is extremely stable even under harsh processing condition (heat, pressure and additives chemicals) and compromised states (natural decomposition) where most protein-based markers are denatured or degraded.
Although several PCR assays have been proposed for feline species detection, those assays are based on longer length target amplicon which are assumed to break down under food processing treatments. Thus, a reliable detection of feline ingredients is crucial for the safety of consumer health, religious faith and fair-trade economy. In this study, a
69-bp target of feline mitochondrial cytochrome b gene was selectively amplified using a pair of primers of the said species. The assay was specific for feline species under raw, processed, admixed and commercial food matrices. The specificity of the developed assay was checked against commercially important 14 terrestrial 5 aquatic and 5 plants species.
The target DNA stability under various food processing conditions such boiling, autoclaving and microwaving that degrade DNA and exceptional constancy were found in all treatments. The lower limit of detection of the assay was reflected by its ability to detect 0.1 pg of feline DNA from raw meats, 0.01% (w/w) in different admixes and 0.1%
(w/w) of feline meats in burger as well as meatball formulations, respectively.
The PCR product was further authenticated by restriction digestion followed by RFLP analysis in microfluidic-based lab-on a chip system. Theoretical analysis revealed two RFLP fragments of length 43 and 26-bp which will be separated using a highly sensitive microfluidic-based lab-on a chip system with a resolution of ≤10-bp. Very short
amplicon-length, extreme stability and high sensitivity suggested that this assay could be used by the regulatory bodies for the routine assessments of feline species in food
forensics or archaeological investigations. Therefore, a short amplicon-length polymerase chain reaction (PCR) was developed and validated it by restriction fragment length
polymorphism (RFLP) analysis for the authentication of feline meat in processed foods
Anti Carcinogenic Effect of Lemon and Lemon Products in Cancer Therapy A Summary of the Evidence
No full textLemon is a popular fruit that includes many helpful nutrients for humans. Accumulated proof from research shows that nutritional intake of lemon and lemon products e.g. lemon peel, lemon grass oil, lemon extract appears to be inversely linked to the reduced danger of numerous infectious illnesses and cancers. These helpful impacts of the lemon will be attributed to their chemical constituents in particular. Lemon contains a range of important nutrients such as vitamin C, vitamin A, carotenes of different kinds, as well as several non nutrient phytochemicals as well as categories of flavonoid, coumarin, glucarate, monoterpenes, triterpenes and phenolic acids. There are many compounds distinctive to lemon that are comparatively uncommon in other plants, their individual components such as sesqui terpene isointermedeol, geraniol, terpene, quercetin and eriocitrin have also shown anticancer activity against various cell lines. This review regularly summarized lemons anticarcinogenic effect in cancer therapy along with the fundamental molecular mechanisms needed to further explore the more efficient use of lemon peel, lemon grass oil, lemon extract. Mohammad Asadul Habib | Kawsar Hossen | Md. Al Amin "Anti-Carcinogenic Effect of Lemon & Lemon Products in Cancer Therapy: A Summary of the Evidence" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-6 , October 2019, URL: https://www.ijtsrd.com/papers/ijtsrd29359.pd
Secondary metabolites from seed extracts of Syzygium cumini (L.)
No full textFour compounds were isolated from the pet-ether and carbon tetrachloride soluble fractions of a methanol extract of seeds of Syzygium cumini. The structures of the isolated compounds were elucidated as 7-hydroxycalamenene (1), methyl-?-orsellinate (2), ?-sitosterol (3) and oleanolic acid (4) through extensive spectroscopic studies, including high-field NMR analyses. This report appears to be the first to identify 7-hydroxycalamenene (1) in S. cumini and the Myrtaceae family, although it has been reported in cultured cells of the liverwort Heteroscyphus planus. This is also the first report of the isolation of compounds 2-4 from this plant species
sj-docx-1-pie-10.1177_09544089221111584 - Supplemental material for Optimization of hydroxyapatite powder mixed electric discharge machining process to improve modified surface features of 316L stainless steel
No full textSupplemental material, sj-docx-1-pie-10.1177_09544089221111584 for Optimization of hydroxyapatite powder mixed electric discharge machining process to improve modified surface features of 316L stainless steel by Mohd Danish, Md Al-Amin, Ahmad Majdi Abdul-Rani, Saeed Rubaiee, Anas Ahmed, Fatema Tuj Zohura, Rasel Ahmed and Mehmet B Yildirim in Proceedings of the Institution of Mechanical Engineers, Part E: Journal of Process Mechanical Engineering</p
sj-docx-2-pie-10.1177_09544089221111584 - Supplemental material for Optimization of hydroxyapatite powder mixed electric discharge machining process to improve modified surface features of 316L stainless steel
No full textSupplemental material, sj-docx-2-pie-10.1177_09544089221111584 for Optimization of hydroxyapatite powder mixed electric discharge machining process to improve modified surface features of 316L stainless steel by Mohd Danish, Md Al-Amin, Ahmad Majdi Abdul-Rani, Saeed Rubaiee, Anas Ahmed, Fatema Tuj Zohura, Rasel Ahmed and Mehmet B Yildirim in Proceedings of the Institution of Mechanical Engineers, Part E: Journal of Process Mechanical Engineering</p
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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