341 research outputs found
Leaky Governance: Alternative Service Delivery and the Myth of Water Utility Independence
No full textBook review by Eva Pip of Leaky Governance: Alternative Service Delivery and the Myth of Water Utility Independence, Kathryn Furlong, author
Efficient Mechanics of PIP Mobilisation Splinting
No full text The proximal interphalangeal (PIP) joint, a tightly constructed hinge joint, frequently develops limited motion following injury. Mobilisation splints are the most frequently used method of regaining PIP joint motion following isolated PIP injury. This article reviews a variety of PIP mobilisation splints, which the author has found effective. In addition to discussing the biomechanics of PIP extension and flexion mobilisation splinting, a variety of designs are offered for PIP extension mobilisation and one design for PIP flexion mobilisation. Clinical problems discussed include: PIP extension lag, PIP flexion contracture responsive to stretch (including acute boutonniere), PIP joint contracture unresponsive to stretch, and gaining/maintaining the last few degrees of PIP extension in a resistive contracture as well as isolated PIP flexion mobilisation. For all splints, construction advice and other tips are given for successful use of these designs. </jats:p
Strategies for Adapting Urban Agriculture to Climate Change: A Priority Action of the Green Cincinnati Plan
No full textRecord ID: 98
Program Affiliation: Capstone
Presentation Type: Video
Abstract: Climate change is affecting the yield, quality, and resiliency of our crops. With The City of Cincinnati already addressing food insecurity through the Green Cincinnati Plan, this Research aims to specifically address the impact, and possible mitigation, of climate change on our agricultural industry. Topics researched include, most at risk crops, responses from other areas within the United States, and possible opportunities for new crops. The outcome of this research will further the strategic response to climate change in our urban agricultural industry, allowing for the possibility of decreased food insecurity, and increased food resiliency in our city. The next steps would be to partner with our local growers to implement these strategies and help improve the resilience of our urban agricultural industry. 
Generation of prolactin-inducible protein (Pip) knockout mice by CRISPR/Cas9-mediated gene engineering
No full textProlactin-inducible protein (PIP) is a multifunctional glycoprotein that is highly expressed and found in the secretions of apocrine glands, such as salivary, lacrimal, and sweat glands including the mammary glands. PIP has been implicated in various diseases, including breast cancer, gross cystic disease of the breast, keratoconus of the eye and the autoimmune Sjgren’s syndrome. Here we have generated a Pip knockout (KO) mouse using the CRISPR/Cas9 system. The Cas9 protein and two single guide RNAs targeting specific regions for both exons 1 and 2 of the Pip gene were microinjected into mouse embryos. The deletions and insertions promoted by CRISPR/Cas9 system on the Pip gene successfully disrupted PIP protein coding, as confirmed by PCR genotyping, sequencing and ultimately Western blot analysis. This mouse model was generated in the inbred C57Bl/6J mouse, which exhibits lower genetic variation. This novel CRISPR Pip KO mouse model will be not only be useful for future studies to interrogate the multifunctional role of PIP in physiological processes but will facilitate a broader understanding of the function of PIP in vivo while providing unprecedented insight into its role in a spectrum of diseases attributed to the deregulation of the PIP gene.The presentation of the authors' names and (or) special characters in the title of the pdf file of the accepted manuscript may differ slightly from what is displayed on the item page. The information in the pdf file of the accepted manuscript reflects the original submission by the author
Everyone Called Him Mister Pip: Great Expectations and Postcolonialism in Lloyd Jones’s Mister Pip
No full textEven though Charles Dickens’s Great Expectations was published roughly 150 years ago, it still has a great impact in literature today. An example of this is the 2006 novel Mister Pip by Lloyd Jones. The novel uses Great Expectations in a unique way. Children on the island of Bougainville read Charles Dickens’s work in school, and the novel starts to play a very large role in the small community, which is being torn apart by a brutal civil war. To answer the question how author Lloyd Jones uses Great Expectations to reflect on postcolonial issues in his novel Mister Pip, it is important to look at several recurring themes that fall under the umbrella term postcolonialism. This thesis will focus on the subjects of mimicry, intertextuality, oral storytelling, rewriting history, and migrating characters. The characters in the novel Mister Pip will be the main focus in the discussion of all of these themes. This thesis uses these elements to answer the previously mentioned research question
Collisional ionization and recombination effects on coalescence instability in chromospheric partially ionized plasmas
No full textThis is the final version. Available on open access from the American Institute of Physics via the DOI in this recordData availability: The data that support the findings of this study are available from the corresponding author upon reasonable request.
The (PIP) code is available at the following url: https://github.com/AstroSnow/PIP. Details of the code and
equations are available in A. Hillier, S. Takasao & N. Nakamura, A&A 591, A112 (2016)54. A copy of the code version
used in this work and the initial condition of the reference simulations can be found in a dedicated repository provided by G.
Murtas: https://github.com/GiuliaMurtas/PIP.gitPlasmoid-mediated fast magnetic reconnection plays a fundamental role in driving explosive dynamics and heating, but relatively little is known about how it develops in partially ionised plasmas (PIP) of the solar chromosphere. Partial ionisation might largely alter the dynamics of the coalescence instability, which promotes fast reconnection and forms a turbulent reconnecting current sheet through plasmoid interaction, but it is still unclear to what extent PIP effects influence this process. We investigate the role of collisional ionisation and recombination in the development of plasmoid coalescence in PIP through 2.5D simulations of a two-fluid model. The aim is to understand whether these two-fluid coupling processes play a role in accelerating reconnection. We find that in general ionisation-recombination process slow down the coalescence. Unlike the previous models in G. Murtas, A. Hillier \& B. Snow, Physics of Plasmas 28, 032901 (2021) that included thermal collisions only, ionisation and recombination stabilise current sheets and suppress non-linear dynamics, with turbulent reconnection occurring in limited cases: bursts of ionisation lead to the formation of thicker current sheets, even when radiative losses are included to cool the system. Therefore, the coalescence time scale is very sensitive to ionisation-recombination processes. However, reconnection in PIP is still faster than in a fully ionised plasma environment having the same bulk density: the PIP reconnection rate () increases by a factor of with respect to the MHD reconnection rate ().Science and Technology Facilities Council (STFC
A novel non-canonical PIP-box mediates PARG interaction with PCNA
No full textPoly(ADP-ribose) glycohydrolase (PARG) regulates cellular poly(ADP-ribose) (PAR) levels by rapidly cleaving glycosidic bonds between ADP-ribose units. PARG interacts with proliferating cell nuclear antigen (PCNA) and is strongly recruited to DNA damage sites in a PAR- and PCNA-dependent fashion. Here we identified PARG acetylation site K409 that is essential for its interaction with PCNA, its localization within replication foci and its recruitment to DNA damage sites. We found K409 to be part of a non-canonical PIP-box within the PARG disordered regulatory region. The previously identified putative N-terminal PIP-box does not bind PCNA directly but contributes to PARG localization within replication foci. X-ray structure and MD simulations reveal that the PARG non-canonical PIP-box binds PCNA in a manner similar to other canonical PIP-boxes and may represent a new type of PIP-box. While the binding of previously described PIP-boxes is based on hydrophobic interactions, PARG PIP-box binds PCNA via both stabilizing hydrophobic and fine-tuning electrostatic interactions. Our data explain the mechanism of PARG–PCNA interaction through a new PARG PIP-box that exhibits non-canonical sequence properties but a canonical mode of PCNA binding.© The Author(s) 201
JPET #166710 PIP BEHAVIORAL INDICES IN ANTIPSYCHOTIC DRUG DISCOVERY Running title: Antipsychotic drug discovery Corresponding Author: Statistics : Number of text pages : 23 Number of tables : 0
No full textABSTRACT Schizophrenia is characterized by three major symptom classes: positive symptoms, negative symptoms and cognitive deficits. Classical antipsychotics (phenothiazines, thioxanthines, butyophenones) are effective against positive symptoms but induce major side-effects, in particular extrapyramidal symptoms (EPS). The discovery of clozapine, which does not induce EPS and is thought effective against all three classes of symptom, has driven research for novel antipsychotics with a wider activity spectrum and lower EPS liability. To increase predictiveness, current efforts aim to develop translational models, where direct parallels can be drawn between the processes studied in animals and in man. The present paper reviews existing procedures in animals for their ability to predict compound efficacy and EPS liability in relation to their translational validity. Rodent models of positive symptoms include procedures related to dysfunction in central dopamine (DA), glutamatergic (N-methyl-D-aspartate, NMDA) and serotonin (5-hydroxytryptamine, 5-HT) neurotransmission. Procedures for evaluating negative symptoms include rodent models of anhedonia, affective flattening and diminished social interaction. Cognitive deficits can be assessed in rodent models of attention (prepulse inhibition) and of learning/memory (object and social recognition, Morris water maze and operant delayed alternation). The relevance of the conditioned avoidance response (CAR) is also discussed. A final section reviews procedures for assessing EPS liability, in particular parkinsonism (catalepsy in rodents), acute dystonia (purposeless chewing in rodents, dystonia in monkeys), akathisia (defecation in rodents) and tardive dyskinesia (long-term antipsychotic treatment in rodents and monkeys). It is concluded that, with notable exceptions (attention, learning /memory, EPS liability), current predictive models for antipsychotics fall short of clear translational validity
An analysis on conflicts faced by the main character of Pip in Charles Dickens’ Great Expectations
No full textAs long as we live in the world, we always faced conflicts. As human beings that have problems attempt to solve it. Conflicts are a contrast or contradiction with two people, or more that have different opinion and ideas. Sometimes conflicts appear when the people have different ideas with other. Conflicts are divided into two parts such as external and internal Conflict, the external conflict is a struggle between a character and an outside force, and character may face several types of outside force like character with community, character with nature. The internal conflict is a struggle that takes place in character’s mind.
Character is a person presented in works of narrative or drama who convey their person qualities though dialogue and action, by which the reader as audience understands their thoughts, feelings, intentions and motives. The study aimed at answering that the following three research problems. First what kinds of Conflicts faced by Pip, Second what are the causes of the conflicts, and the last what are the impacts of Pip’s conflicts in life. This study use structural approach, structural approach which used to analyze
fictions anatomic structure or author, imagination that is independent from other aspect of human life.
The result of the research shows that the main character Pip in Great Expectations by Charles Dickens face two conflicts that are external and internal conflicts; the external conflict included physical and psychological conflicts that faced Pip in his life. Physical conflict happen when Pip fought with slouching man he is name Orlick that dangerous person that hurting people simple because he enjoys it, and the second is when Pip mets the Convict in the Marshes asks some food and a file, if Pip did not give it he Convict will cut Pip’s throat. It this happen that makes Pip felt afraid if he mets again with the Convict or strange man, and the psychological conflict when Estella insults him with called labouring and poor boy. In pip’s thought he want to be a gentleman in London and to similarity with Estella’s dignity, and the most important of this Pip want to get Estella’s love, but Pip not get it. After all of this events Pip’s realize and it is can make Pip encourage to better than before in his life. The Internal conflict Pip’s life so make Pip realize that all of his want not always appropriate what his want in real life
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