1,720,969 research outputs found
Subsampled fastq from GSM7890929 and GSM7890951
No full text<p>This record contains:<br>- 4 fastqs which are subsets of fastqs corresponding to mESC and 168h gastruloids (the whole fastqs are available at SRA).<br>- All command lines which have been used to generate these fastqs:<br> - The main script is pipeline.sh<br> - Other files are temporary files or accessory scripts<br><br>The subsetting is totally biased and promote cells from different clusters so the proportion are absolutely artifical!</p><p>The goal of this subsetting is to be able to use this data in training and workflow testing.</p>
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
Interplay between chromatin and transcription factors drives pituitary cell fate specification
No full textComplex organisms begin as a single undifferentiated cell, the zygote, which derives a multitude of highly specialized cell types through sequential rounds of differentiation. Cell fate specification is the fundamental process by which cells engage towards specialization. Cell fate transitions during normal development are poorly understood. My work focused on the role of pioneer factors during differentiation. These transcription factors find their target sites even concealed in closed chromatin leading to chromatin opening and binding of nonpioneer transcription factors.We first studied pioneer driven cell differentiation of two pituitary lineages, melanotropes and corticotropes, that both express the pro-opiomelanocortin (POMC) gene. We showed that Pax7 pioneer action drives melanotrope specification in vivo by opening a new enhancer repertoire. We uncovered pioneer action dynamics using an engineered inducible Pax7. Pax7 can locate its target rapidly (less than 30 minutes) but initially binds weakly. Then, Pax7 binds strongly in less than 24h while chromatin opening is slow and progressive over more than three days. Following Pax7 withdrawal, long-term memory of pioneer action is associated with loss of DNA methylation.We then, investigated the role of nonpioneers during pioneer driven chromatin opening. The typical model of pioneer factor action assumes that nonpioneers are passive in this process and that they opportunistically bind newly accessible chromatin, yet this was never tested. The pioneer factor Pax7 acts as the specifying factor for melanotropes fate while the nonpioneer Tpit acts as the determining factor of both melanotropes and corticotropes. Chromatin accessibility is affected in Pax7 or Tpit deficient mice; they are required for the opening of their cognate program. Strikingly, in the absence of Tpit, Pax7 fails to drive melanotrope chromatin opening. Ectopically expressed Pax7 confirmed that Pax7 bind closed chromatin regardless of Tpit expression while Tpit is unable to bind closed chromatin in absence of Pax7. However, in the absence of Tpit, Pax7 does not open chromatin. In summary, we propose that cooperation between a pioneer factor and a nonpioneer factor drives lineage specific chromatin opening.Tous les organismes multicellulaires débutent par le zygote, une cellule non différenciée qui dans le développement embryonnaire se différencie en multiples types cellulaires spécialisés. La spécification du destin cellulaire est un processus fondamental par lequel la cellule s'engage en différenciation. Les transitions de destin cellulaire durant le développement sont mal comprises. Mon travail s'est concentré sur le rôle des facteurs pionniers durant la différenciation. Ces facteurs sont capables de trouver leurs cibles même lorsque l'ADN est inaccessible dans de la chromatine fermée et permettent l'ouverture de la chromatine et la liaison de facteurs non-pionniers.D'abord, nous avons étudié l'implication de l'action pionnière lors de la différenciation de deux lignées hypophysaires, les mélanotropes et les corticotropes, qui expriment toute deux le gène de la pro-opiomelanocortine. Nous avons montré que l'action pionnière de Pax7 dirige la spécification des mélanotropes in vivo en ouvrant un nouveau répertoire d'enhancers. Nous avons découvert la dynamique de l'action pionnière à l'aide d'une version inductible de Pax7. Pax7 trouve ses cibles rapidement (moins de 30 minutes) mais initialement les lie faiblement. Pax7 est ensuite stabilisé sur ses sites en moins de 24h alors que l'ouverture de la chromatine est lente et progressive sur plus de trois jours. Après retrait de Pax7, l'action pionnière de Pax7 est stable et associées à une perte de la méthylation de l'ADN.Nous avons ensuite étudié le rôle des facteurs non-pionniers dans l'ouverture de la chromatine dépendante des facteurs pionniers. Le modèle classique de l'action pionnière présume que les non-pionniers sont passifs dans le processus et lient la chromatine nouvellement accessible de manière opportuniste, mais cela n'a jamais été testé. Le facteur pionnier Pax7 agi comme spécificateur du destin mélanotrope alors que le facteur non pionnier Tpit agi comme facteur de détermination pour les mélanotropes et les corticotropes. L'accessibilité de la chromatine est affectée dans les souris déficientes pour Pax7 ou Tpit, chacun est requis pour l'ouverture de son programme respectif. De manière remarquable, Pax7 ne déclenche pas l'ouverture de ses régions cibles en l'absence de Tpit. L'expression ectopique de Pax7 en présence/absence de Tpit confirme que Pax7 lie de manière stable la chromatine fermée en présence/absence de Tpit alors que Tpit ne lie pas la chromatine fermée en l'absence de Pax7. En revanche, en l'absence de Tpit, la liaison de Pax7 ne permet pas l'ouverture de la chromatine. Nous proposons qu'une coopération entre pionniers et non-pionniers permet l'ouverture de la chromatine
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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