72 research outputs found
Collection des Auteurs Classiques François et Latins
No full textBodemann seems to say that Didot did a major four-volume illustrated edition of this very size (about 3 x 5) in 1787. This present two-volume set may be the texts of that edition arranged now as part of a series: Collection des Auteurs Classiques François et Latins. The title-page also adds Imprimé par Ordre du Roi pour l'Éducation de Monseigneur le Dauphin. This second volume contains Books VII through XII, with an AI at the book's end. Calf binding and gilt page edges. A lovely little set of volumes, up to the high standards which the commissioning letter by Amelot in the first pages sets.This is a hardbound book (hard cover)Language note: FrenchJean de La Fontain
Suivi des patients implantés par un défibrillateur automatique implantable sans fonction de resynchronisation en prévention primaire de cardiomyopathies ischémiques et dilatées non ischémiques de 2005 à 2007 au CHU de Caen
No full textCAEN-BU Médecine pharmacie (141182102) / SudocSudocFranceF
Collection des Auteurs Classiques François et Latins
No full textBodemann seems to say that Didot did a major four-volume illustrated edition of this very size (about 3 x 5) in 1787. This present two-volume set may be the texts of that edition arranged now as part of a series: Collection des Auteurs Classiques François et Latins. The title-page also adds Imprimé par Ordre du Roi pour l'Éducation de Monseigneur le Dauphin. This first volume contains Books I through VI, Preliminary material runs through some 115 pages with Roman numerals. Calf binding and gilt page edges. A lovely little set of volumes, up to the high standards which the commissioning letter by Amelot in the first pages sets.This is a hardbound book (hard cover)Language note: FrenchJean de La Fontain
Analyse Multi-Echelle des Lisières pour la réalisation de la trame verte de la région Poitou- Charentes – Expérimentation d’ateliers de cartographie participative du bocage bressuirais
No full textInternational audienc
Autonomie d'un diplomate ou prise de risque : Amelot de Gournay et l'abolition des fueros en 1707
No full textInternational audienc
Propagation and titration of infectious bursal disease virus, including non-cell-culture-adapted strains, using ex vivo-stimulated chicken bursal cells
No full textABSTRACT
Infectious bursal disease virus (IBDV) is a Birnaviridae family member of economic importance for poultry. This virus infects and destroys developing B lymphocytes in the cloacal bursa, resulting in a potentially fatal or immunosuppressive disease in chickens. Naturally occurring viruses and many vaccine strains are not able to grow in in vitro systems without prior adaptation, which often affects viral properties such as virulence. Primary bursal cells, which are the main target cells of lymphotropic IBDV in vivo, may represent an attractive system for the study of IBDV. Unfortunately, bursal cells isolated from bursal follicles undergo apoptosis within hours following their isolation. Here, we demonstrate that ex vivo stimulation of bursal cells with phorbol 12-myristate 13-acetate maintains their viability long enough to allow IBDV replication to high titres. A wide range of field-derived or vaccine serotype 1 IBDV strains could be titrated in these phorbol 12-myristate 13-acetate -stimulated bursal cells and furthermore were permissive for replication of non-cell-culture-adapted viruses. These cells also supported multistep replication experiments and flow cytometry analysis of infection. Ex vivo-stimulated bursal cells therefore offer a promising tool in the study of IBDV
Blood B Cell Depletion Reflects Immunosuppression Induced by Live-Attenuated Infectious Bursal Disease Vaccines
No full textImmunosuppression in poultry production is a recurrent problem worldwide, and one of the major viral immunosuppressive agents is Infectious Bursal Disease Virus (IBDV). IBDV infections are mostly controlled by using live-attenuated vaccines. Live-attenuated Infectious Bursal Disease (IBD) vaccine candidates are classified as “mild,” “intermediate,” “intermediate-plus” or “hot” based on their residual immunosuppressive properties. The immunosuppression protocol described by the European Pharmacopoeia (Ph. Eur.) uses a lethal Newcastle Disease Virus (NDV) infectious challenge to measure the interference of a given IBDV vaccine candidate on NDV vaccine immune response. A Ph. Eur.-derived protocol was thus implemented to quantify immunosuppression induced by one mild, two intermediate, and four intermediate-plus live-attenuated IBD vaccines as well as a pathogenic viral strain. This protocol confirmed the respective immunosuppressive properties of those vaccines and virus. In the search for a more ethical alternative to Ph. Eur.-based protocols, two strategies were explored. First, ex vivo viral replication of those vaccines and the pathogenic strain in stimulated chicken primary bursal cells was assessed. Replication levels were not strictly correlated to immunosuppression observed in vivo. Second, changes in blood leukocyte counts in chicks were monitored using a Ph. Eur. - type protocol prior to lethal NDV challenge. In case of intermediate-plus vaccines, the drop in B cells counts was more severe. Counting blood B cells may thus represent a highly quantitative, faster, and ethical strategy than NDV challenge to assess the immunosuppression induced in chickens by live-attenuated IBD vaccines
Propagation and titration of infectious bursal disease virus, including non-cell-culture-adapted strains, using<i>ex vivo</i>-stimulated chicken bursal cells
No full text
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