102,007 research outputs found
A broad host range reporter plasmid for the analysis of divergent promoter regions
No full textCITATION: Jiwaji, M., Matcher, G. F. & Dorrington, R. A. 2008. A broad host range reporter plasmid for the analysis of divergent promoter regions. South African Journal of Science, 104:305-307.The original publication is available at https://www.sajs.co.zaAlthough many vectors exist for Escherichia coli and closely related species, there are few broad host range vectors that can be conjugated into a large variety of Gram-negative bacteria. We have constructed a broad host range vector, pMJ445, that facilitates the analysis of divergent promoters in Gram-negative bacteria. The vector was validated using two intergenic regions derived from gene clusters involved in hydantoin hydrolysis, from the environmental isolates Pseudomonas putida and Agrobacterium tumefaciens. The DNA sequences analysed were capable of activating expression of the reporter enzymes, β-glucuronidase and β-galactosidase, present on pMJ445, indicating the presence of divergent promoters in the sequences selected. In addition, we demonstrated that pMJ445 can be applied to gene regulation studies.Publisher's versio
Bibliographie Hilarion G. Petzold 1958 – 2009 mit Anhang als Einführung
No full textDieses Archiv enthält die Gesamtbibliographie der Werke des Autors nebst einiger Texte „Über H. G. Petzold“ im Schlussteil der Bibliographie sowie einen Anhang mit einer Einführung in die Architektur des Werkes in seinem wissenslogischen Aufbau als Ausarbeitung seines „Tree of Science Modells“ (2007).This archive contains the complete bibliography of the author and some texts about H. G. Petzold, moreover an epilogue with an introduction to the architecture of the works in its epistemological structure and composition and as an elaborations of Petzold’s „Tree of Science Modell (2007).https://www.fpi-publikation.de/polyloge/01-2009-petzold-h-g-gesamtbibliographie-h-g-petzold-1958-2009-updating-november2009/peerReviewedpublishedVersio
Dispelling the Myths Behind First-author Citation Counts
No full textWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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3346: Samuel G. Freedman, author, 2013
No full textPhotograph of author Samuel G. Freedman, at NT Daily Slash meeting in the Mayborn School of Journalism at UNT
Application of Nanomagnetic Logic: Co/Pt based Systolic Pattern Matcher
No full textNanomagnet Logic (NML) is widely considered to be one of the promising for “beyond-CMOS” nanoscale architectures. So far only relatively simple circuits (nanomagnetic logic gates and adders) have been studied experimentally and in simulations. Here we investigate the possibility of building larger-scale computing devices from out-of-plane NML. We designed a systolic pattern matcher circuit that is in principle scalable to arbitrary number of nanomagnets and can match arbitrarily long patterns in an incoming data stream. The design of this systolic architecture for NML makes an important step toward large-scale devices
Computer simulation of the skin reflectance spectra
No full textThe reflectance spectra of the human skin in visible and near-infrared (NIR)
spectral region have been calculated using the Monte Carlo technique, and the
specular and internal reflection on the medium surface is taken into account.
Skin is represented as a complex inhomogeneous multi-layered highly scattering
and absorbing medium. The model takes into account variations in spatial
distribution of blood, index of blood oxygen saturation, volume fraction of
water and chromophores content. The simulation of the skin tissues optical
properties and skin reflectance spectra are discussed. Comparison of the results
of simulation and in vivo experimental results are given
Quantitative assessment of skin layers absorption and skin reflectance spectra simulation in the visible and near-infrared spectral regions
No full textWe have simulated diffuse reflectance spectra of skin by assuming a wavelength-independent scattering coefficient for the different skin tissues and using the known wavelength dependence of the absorption coefficient of oxy- and deoxyhaemoglobin and water. A stochastic Monte Carlo method is used to convert the wavelength-dependent absorption coefficient and wavelength-independent scattering coefficient into reflected intensity. The absorption properties of skin tissues in the visible and near-infrared spectral regions are estimated by taking into account the spatial distribution of blood vessels, water and melanin content within distinct anatomical layers. The geometrical peculiarities of skin histological structure, degree of blood oxygenation and the haematocrit index are also taken into account. We demonstrate that when the model is supplied with reasonable physical and structural parameters of skin, the results of the simulation agree reasonably well with the results of in vivo measurements of skin spectra.Physiolgical Measuremen
The Right to Strike under the United States Constitution: Theory, Practice, and Possible Implications for Canada
No full textAnswering critics of the Canadian Supreme Court's judgment in B.C. Health, the author argues that the Court laid the foundation for a principled and durable doctrine protecting constitutional labour rights, one that goes directly to the heart of the matter — the inequality of workers’ power in the employment relation. In the author’s view, two paths could lead from B.C. Health to the recognition of Charter protec- tion for a right to strike: one that treats the right as an accessory to col- lective bargaining, and one that upholds the right directly on the basis of the Charter values of equality and participation. The author supports the latter approach, contending that constitutional rights should be defined in relation to fundamental values, in a way that is not contingent on time-bound or fact-sensitive assessments about the role of strikes within a particular collective bargaining regime. Although a Charter right to strike may involve the courts in difficult choices about when to defer to legislative policy decisions, and courts may lack the institutional capac- ity to deal effectively with labour law issues, the author points out that judges can look to ILO standards for expert guidance. Noting that the U.S. experience in this area might be of considerable use to Canadians, the author concludes by providing an overview of American case law concerning a constitutional right to strike.Peer reviewe
G-Rank: Unsupervised Continuous Learn-to-Rank for Edge Devices in a P2P Network
No full textRanking algorithms in traditional search engines are powered by enormous training data sets that are meticulously engineered and curated by a centralized entity. Decentralized peer-to-peer (p2p) networks such as torrenting applications and Web3 protocols deliberately eschew centralized databases and computational architectures when designing services and features. As such, robust search-and-rank algorithms designed for such domains must be engineered specifically for decentralized networks, and must be lightweight enough to operate on consumer-grade personal devices such as a smartphone or laptop computer. We introduce G-Rank, an unsupervised ranking algorithm designed exclusively for decentralized networks. We demonstrate that accurate, relevant ranking results can be achieved in fully decentralized networks without any centralized data aggregation, feature engineering, or model training. Furthermore, we show that such results are obtainable with minimal data preprocessing and computational overhead, and can still return highly relevant results even when a user’s device is disconnected from the network. G-Rank is highly modular in design, is not limited to categorical data, and can be implemented in a variety of domains with minimal modification. The results herein show that unsupervised ranking models designed for decentralized p2p networks are not only viable, but worthy of further research.https://github.com/awrgold/G-RankComputer Scienc
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