43 research outputs found
Characterisation of cucumber mosaic virus (CMV) in Catharanthus roseus (L.)G. don and its effects on anticancer metabolite production
Get PDFNatural virus-like disease symptoms such as mosaic and deformation of the leaves and flowers of malformed shape or slight colour-breaking of the petals were observed on Catharanthus roseus plants in Serdang, Selangor, Malaysia. Double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA) detected cucumber mosaic virus (CMV) in high concentrations in the leaf extract of naturally-infected C.
roseus as well as in the leaf extract of inoculated C. roseus. The purified virions were isometric particles with mean diameter 28.60 ± 0.48 nm and contained a central core. The virus induced systemic leaf mosaic on N. tabacum cv. White Burley, C. sativus, N. benthamiana and N. glutinosa. Local lesions and brown necrotic local lesions were
produced on the inoculated leaves of C. amaranticolor and V.sesquipedalis, respectively. A 1000 bp DNA fragment covering the entire coat protein (CP) region of the purified virus was amplified using the primers CMVF1(5’-TAGACAT/ACTGTGACGCGA-3’) and CMVR2 (5’-GTAAGCTGGATGGACAAC-3’). The sequence obtained (GenBank accession number EU726631) revealed a 100% nucleotide and amino acid identities to a CP gene of CMV isolated from C. roseus in India (GenBank accession number EU310928) which is a member of subgroup 1B. Cytopathological study showed that CMV infection disrupted the chloroplast ultrastructure in the C. roseus leaf cells. The presence of large starch grains in the surrounding necrotic zones caused disintegration of the stromatic lamellae and grana, changing the chloroplasts symmetry. Complex membranous structures in the cells
vacuoles and phytoferritin macromolecules in the chloroplast stroma were also observed in the leaf cells of CMV-infected C. roseus. Chloroplast was also the most altered organelle following CMV infection in the leaf cells of N. tabacum cv. White Burley, C. sativus and C.
amaranticolor. A comparative HPLC analysis on the yields of two anticancer metabolites, vincristine and vinblastine in the C. roseus leaf,stem and root tissues at different growing stages indicated that CMV infection modified the metabolism of the metabolites. Following CMV infection, the peak production period of vincristine and vinblastine in
leaves was delayed from four months old uninfected C. roseus plants to six months old CMV-infected plants. CMV infection also delayed the peak production period of vincristine in roots from five months old uninfected plants to seven months old infected ones. As for vinblastine,
the peak production period was delayed from five months old uninfected plants to eight months old infected plants. Another remarkable alteration after CMV infection is that significant increases in the contents of vincristine in the infected root tissues, particularly at six to nine
months old. The concentration of vinblastine which increased about two, four and seven-folds in the roots of six, seven and eight months old CMV-infected plants, respectively, could be another interesting finding
of CMV modification, particularly in these anticancer compounds biosynthesis pathway in C. roseus plants
Modern Arabic literary biography : a study of character portrayal in the works of Egyptian biographers of the first half of the twentieth century, with special reference to literary biography
Get PDFIn Chapter one, I presented a comparative definition of the meaning of Sirah (PI.Siyar), Tarjamah (Pl. Tarajim), Manaqib, Tabaqat and Maghazi as they were understood in antiquity. I also showed how the meaning of Sirah in modern times has only narrowly developed. Although the method of biographical writing continuously developed in Europe, it hardly progressed in Modem Arabic Literature. The only exception was seen in the writings by the pioneers of enlightenment in Egypt at the beginning of the twentieth century. This change of direction relied on borrowing European methodology in biographical writing.
In chapter two, I reviewed the early attempts at writing biographies in the nineteenth century by Abd al Rahman al- Jabarti and Ali Mubarak. Although both were the first
pioneers in this respect, yet they followed the footpath of classical approach above all that of al-Maqarizi from whom -Ali Mubarak derived inspiration in his book Al-Khitat al-Tawfiqiyyah.
In chapter three, I studied the twentieth century, starting with traditional biography writers who could not employ European methodologies and whose writings oscillated between biographical notes and biographical sketches; or whose texts were more of a literary study than a biography proper.
In chapters four to nine, I selected the most renowned, productive writers who best represented methodologies of biography writing. Perhaps certain writers have not
been mentioned in this period of study. This is not out of negligence but simply because their texts were totally out of reach, or their writings did not exhibit the
required literary criteria.
All methodologies representing the theory of biography writing in Egypt have been analysed in these chapters. All, in fact, form a digestion or assimilation of French,English and German schools. In Egypt, Taha Husayn is considered the chairman of the French school, al-Mazini and al-Aqqad of the English/German schools, al-Nuwaihi of the psychoanalytical/anatomical school and Sidqi who employed both. By contrast, al Iryan was the trailblazer of the distinguished biographical novel.
In these chapters, I tried to lay out the general outlines these writers have produced in the production of biographical texts, and how these attempts were a successful step on the road of presenting literary biographies characterized by high world standards.
Chapter ten may well seem traditional, but it is important to give a comparative outlook on the views of biography writers themselves when they study and analyse
the same character.
Among the characters studied ,I selected Bashsliar, Abu Nuwas, Ibn al-Run-i, al-Mutanabbi and al-Maarri. These are outstanding landmarks in the history of Arab verse and the subject of a multitude of studies as well. Modern biographers took these figures as a test field for the deployment and employment of biographical methodologies. I selected these examples to provide comparisons and explain how far these biographies were successful in producing a biography or a profile of those classical poets.
The conclusion and the bibliographical list arrived at the end of research.
I wish, however, to clarify one important point here. It seems that I could not fix the year 1950 as the temporal parameter of my research but took some textswhich were
published shortly beyond that point. The reason for this obvious extension was either to give additional useful details or simply because chapters of such texts had already
been published prior to that year and were known to the readership.
At times I would satisfy myself with analysing the part rather than the whole. This again was meant to eschew repetition or was due to the fact that the book in question
was not available
Use of reverse transcription-polymerase chain reaction (RT-PCR) for Cymbidium mosaic virus (CyMV) detection in orchids
Get PDFThe reverse transcription-polymerase chain reaction CRT-PCR) was
adapted for detection of Cymbidium mosaic virus CCyMV) in orchids.
The oligonucleotide primers used were selected from the predicted
homologous coat protein region of CyMV and other Potexviruses
which enabled to amplify approximately 313 bp and 227 bp fragments
using optimum reaction conditions of 2.5 mM MgCh and 30 cycles of
amplification. The RT-PCR allowed the detection of CyMV RNA and virion in
purified fonns as well as in crude tissue extracts of orchid. Direct
CyMV RNA detection was possible in leaves, shoots, stems, roots and
petals. The detection limits of RNA in purified CyMV and virion by
RT-PCR described were 10 ng and 2 ng, respectively. The PCR
amplified fragments were confinned to be CyMV-specific by dotblot
hybridization with DIG-labelled CyMV cDNA probe.
The suitability of the RT-PCR in routine testing of CyMV was
detennined and compared with those of DAS-ELISA. Thirty samples
of leaf tissues representing various genera or hybrids of cultivated
local orchid from glasshouse and commercial nurseries were tested
for CyMV by RT-PCR and DAS-ELISA. Among 15 samples that
tested positive for CyMV infection by DAS-ELISA, only 7 samples
gave the expected amplification fragments when subjected in RTPCR
assays. The equal detection limit on purified CyMV virion by
RT-PCR and DAS-ELISA and lower sensitivity of RT-PCR in
detecting CyMV in a field indexing trial suggested that RT-PCR is
unsuitable to replace DAS-ELISA for routine testing of CyMV in
local orchids
Prediction of settlement by using finite element simulation 2D program at Seksyen 7, Shah Alam
No full textSynthesis and characterization of oleic acid surface modified magnetic iron oxide nanoparticles by using biocompatible w/o microemulsion for heavy metal removal
No full textFactors Affecting the Performance of Small Business Start-Ups Under Tunas Mekar Programme
No full textA review for improving software change using traceability model with test effort estimation
No full textMaintaining a software system includes tasks such as fixing defects, adding new features, or modifying the software (software changes) to accommodate different environments. Then, the modified software system needs to be tested, to ensure the changes will not having any adverse effects on the previously validated code. Regression testing is one of the approaches which software tester used to test the software system. The traditional regression testing strategy was to repeat all the previous tests and retesting all the features of the program even for small modifications. For programming with thousand lines of codes (LOC), the cost of retesting the entire system is expensive if attempted after every change. This practice is becoming increasingly difficult because of the demand for testing the new functionalities and correcting errors with limited resources. Numerous techniques and tools have been proposed and developed to reduce the costs of regression testing and to aid regression testing processes, such as test suite reduction, test case prioritization, and test case done on the thresholds and weightings used in regression testing. However, there is still need to study on the software traceability model of coverage analysis in software changes during regression testing and test effort estimation on regression testing. Hence, this paper describes the proposal for improving software changes with hybrid traceability model and test effort estimation during regression testing. We will explain our proposed work including the problem background, the intended research objectives, literature review and plan for future implementation. This study is expected to contribute in developing hybrid traceability model for large software development project to support software changes during regression testing with test estimation approach and expected to reduce operational cost during the implementation on software maintenance. Also, it is hoped that an efficient and improve solution to regression testing can be realized, thus, gives the benefits to software testers and project manager manage the software maintenance task since it is a critical part in software project development
Fuzzy Delphi method for evaluating HyTEE model (hybrid software change management tool with test effort estimation)
Get PDFWhen changes are made to a software system during development and maintenance, they need to be tested again i.e. regression test to ensure that changes behave as intended and have not impacted the software quality. This research will produce an automated tool that can help the software manager or a maintainer to search for the coverage artifact before and after a change request. Software quality engineer can determine the test coverage from new changes which can support cost estimation, effort, and schedule estimation. Therefore, this study is intended to look at the views and consensus of the experts on the elements in the proposed model by benefitting the Fuzzy Delphi Method. Through purposive sampling, a total of 12 experts from academic and industrial have participated in the verification of items through 5-point linguistic scales of the questionnaire instrument. Outcome studies show 90% of elements in the proposed model consists of change management, traceability support, test effort estimation support, regression testing support, report and GUI meet, the value threshold (d construct) is less than 0.2 and the percentage of the expert group is above 75%. It is shown that elements of all the items contained in the venue are needed in the HyTEE Model (Hybrid Software Change Management Tool with Test Effort Estimation) based on the consensus of experts
Use of Polymerase Chain Reaction (PCR) for Cymbidium Mosaic Virus (CYMV) detection and a comparison with DAS-ELISA
Get PDFPrestasi perusahaan kecil di bawah program Tunas Mekar: analisis di peringkat awal / Najihah Marha Yaacob ... [et al.]
Get PDFKerajaan telah menyediakan pelbagai program sokongan untuk meningkatkan penglibatan kaum Bumiputera dalam sektor moden. Tunas Mekar merupakan salah satu program kerajaan untuk melahirkan lebih ramai usahawan Bumiputera daripada kalangan graduan. Meskipun pelbagai kemudahan disediakan samada dalam bentuk kredit atau bukan kewangan, namun hakikatnya penglibatan golongan graduan dalam bidang perniagaan masih lagi berkurangan. Di kalangan mereka yang terlibat juga jelas menunjukkan prestasi dan penguasaan yang masih lagi ketinggalan berbanding dengan pencapaian kaum-kaum lain. Objektif kajian ini adalah untuk melihat faktor-faktor yang boleh mempengaruhi prestasi perusahaan kecil yang diusahakan oleh graduan pada peringkat awal operasi. Berdasarkan analisis regresi berganda, dapatan kajian menunjukkan faktor ciri-ciri keusahawanan, amalan pengurusan serta latihan dan bimbingan mempunyai pengaruh yang signifikan ke atas prestasi perusahaan kecil di peringkat awal operasi. Manakala faktor pengalaman perniagaan dan jumlah modal permulaan didapati tidak signifikan untuk menerangkan prestasi perusahaan kecil pada peringkat tersebut, Dapatan kajian ini bukan sahaja memberi implikasi ke atas Teori Firma dan Teori Modal Insan, tetapi juga memberi implikasi ke atas pengusaha kecil, penggubal dan pelaksana polisi serta penyelidik. Beberapa cadangan dikemukakan termasuklah program pembangunan diri, meningkatkan kecekapan pengurusan perniagaan menerusi latihan dan bimbingan, program pendedahan dan pemantauan serta menyediakan kemudahan kredit. Bagi kajian masa hadapan, beberapa faktor lain yang dilihat berpotensi seperti sumber kewangan, faktor ekonomi, sokongan kerajaan dan jaringan perniagaan perlulah dimasukkan dalam analisis kajian bagi mendapat gambaran yang lebih menyeluruh tentang faktor-faktor yang mempengaruhi prestasi perusahaan kecil di peringkat awal operasi
