13 research outputs found
STERILITA': NUOVO APPROCCIO MOLECOLARE E CITOGENETICA. ANALISI DELLA LETTERATURA
No full textIn the therapeutic procedure of conjugal fertility, the use of intracytoplasmic sperm injection (ICSI) has opened new possibilities when conventional in vitro fertilization (IVF) techniques are unsuccessful. Cytogenetic studies showed the presence of genetic anomalies in foetuses conceived with this technique. Starting from these considerations, through a data collection of the literature, the authors report the latest scientific findings on this problem. They show the limits of the knowledge about genetic risks linked to these techniques, increasingly imposing rigorous medical behavior and conscious choices of the couple
Diacylglycerol kinase-alpha mediates hepatocyte growth factor-induced epithelial cell scatter by regulating Rac activation and membrane ruffling
No full textDiacylglycerol kinases (Dgk) phosphorylate diacylglycerol (DG) to phosphatidic acid (PA), thus turning off and on, respectively, DG-mediated and PA-mediated signaling pathways. We previously showed that hepatocyte growth factor (HGF), vascular endothelial growth factor, and anaplastic lymphoma kinase activate Dgkalpha in endothelial and leukemia cells through a Src-mediated mechanism and that activation of Dgkalpha is required for chemotactic, proliferative, and angiogenic signaling in vitro. Here, we investigate the downstream events and signaling pathways regulated by Dgkalpha, leading to cell scatter and migration upon HGF treatment and v-Src expression in epithelial cells. We report that specific inhibition of Dgkalpha, obtained either pharmacologically by R59949 treatment, or by expression of Dgkalpha dominant-negative mutant, or by small interfering RNA-mediated down-regulation of endogenous Dgkalpha, impairs 1) HGF- and v-Src-induced cell scatter and migration, without affecting the loss of intercellular adhesions; 2) HGF-induced cell spreading, lamellipodia formation, membrane ruffling, and focal adhesions remodeling; and 3) HGF-induced Rac activation and membrane targeting. In summary, we provide evidence that Dgkalpha, activated downstream of tyrosine kinase receptors and Src, regulates crucial steps directing Rac activation and Rac-dependent remodeling of actin cytoskeleton and focal contacts in migrating epithelial cell
Legislar pela exclusão social: um estudo da atividade legislativa sobre cumprimento da pena de 1984 a 2011
No full textThe main objective of this dissertation is to understand the Legislative activities and your interaction among Judiciary and Executive in terms of enforcement of criminal sanction. The considered assumption is that the process of creation of laws about sanction management in Brazil is recurrent the selection of prisons sentences, and the management way created by LEP (Law of Penal Execution) is subject of few proposals of legislative reform. This study includes an empirical research of legislative proposals presented to National Congress between 1984 and 2011 related to the enforcement of criminal sanction. The second chapter of the dissertation contains a quantitative analysis of these proposals, through the systematization of general data (type of proposition, length of the procedure for approval, federal state of origin and author) to identify, when possible, the increase or decrease trend of social exclusion through the use of more or less frequence of imprisonment. The qualitative analysis focus on the concepts of management of criminal sanction and individualization of punishment. The third chapter introduces the concept of management of criminal sanction, review of legislative historical creation of legal degree to accomplishment of sanction until the creation of LEP in 1984, and reinforces a qualitative study of task divisions about accomplishment of sanction from an empirical material. The fourth chapter introduces the idea of individualization of sanction, as approached in a judicial activity, which interacts in a dynamic and constant way with legislative and administrative activities, especially while the accomplishment of prison sentencing. From this concept, new legislative proposals present a new arrangement of distribution of tasks among the three powers regarding enforcement of criminal sanction. The conclusion of this dissertation is that the creation process of criminal laws in Brazil recognizes the use of freedom private sanction, considering the extension of minimum prison quorum or the extinction of subjective rights, in a situation that the judge has no many possibilities to actuate in case.A presente dissertação tem por objetivo compreender a atividade legislativa e a articulação entre os Poderes Legislativo, Judiciário e Executivo no momento de cumprimento da pena pelo condenado. Parte-se da hipótese de que no processo de criação de leis sobre gestão da sanção no Brasil é recorrente a seleção de penas prisionais e que a atividade de determinação da quantidade e da qualidade da pena no caso concreto é objeto de disputa entre legislador, juiz e administrador penitenciário. Para investigar esta hipótese, a pesquisa comporta um levantamento empírico de proposições legislativas apresentadas no Congresso Nacional entre 1984 e 2011 relacionadas ao cumprimento da sanção criminal. O segundo capítulo da dissertação contém uma análise quantitativa destas proposições, por meio da sistematização dos dados gerais (tipo de proposição, tramitação, estado de origem e autor) e identifica, quando possível, as medidas tendentes a ampliar ou reduzir a exclusão social por meio do uso mais ou menos frequente da pena de prisão. O terceiro capítulo introduz o conceito de gestão da sanção, revisa o histórico legislativo de criação de um diploma legal voltado ao cumprimento da pena até a criação da LEP (1984) e empreende um estudo qualitativo sobre a divisão de tarefas sobre cumprimento da pena a partir do material empírico. O quarto capítulo apresenta a ideia de individualização da pena, conceito abordado da perspectiva de uma atividade judicial que interage de forma dinâmica e constante com as atividades legislativas e administrativas, especialmente durante o cumprimento da pena de prisão. A partir deste conceito são retomadas proposições legislativas que, em alguma medida, abordam o arranjo ou o rearranjo da divisão de tarefas entre os três poderes. Ao final, conclui-se que o processo de elaboração de normas penais no Brasil valoriza o uso da pena de privativa de liberdade, por meio da ampliação das frações mínimas prisionais ou da vedação de direitos subjetivos, situação na qual o juiz tem pouco espaço para atuar no caso concreto
Diacylglycerol Kinase-{alpha} Mediates HGF-induced Epithelial Cell Scatter by Regulating Rac Activation and Membrane Ruffling.
No full text
Playing Soldiers: Posing Militarism in the Domestic Sphere
No full textIn “Playing Soldiers: Posing Militarism in the Domestic Sphere”, Pasternak explores the political life of family photographs and the social rituals attending the practice of family photography in an attempt to broaden and politicise scholarly literature on this subject. Focusing on Israeli society, Pasternak’s contribution delves into the micro-politics of family photography practiced specifically by Jewish-Israelis in the context of the Arab-Zionist conflict that developed after the formal establishment of the Israeli state in 1948.
Pasternak presents an unconventional approach to family photography by addressing images selected from collections belong to his own family. This approach epitomises his desire to develop a conceptual framework distinct from conventional art historical approaches. Pasternak argues for the need to study the photographic apparatus beyond the traditional parameters of dominant social taxonomies and their prescribed significances. In doing so, he questions the common understanding of the family photograph as apolitical.
Pasternak’s contribution examines portraits of members of his family imaged while serving in the Israeli Defence Forces (IDF). Whereas there is nothing unusual in producing and accumulating family photographs of soldiers, it is a practice, he argues, that reveals the lack of clear boundaries between the state, society, and the nuclear family. In a country such as Israel, where militarism and violent political conflict are part and parcel of the everyday, posing militarism in the domestic sphere appears as necessary. Pasternak introduces sociological and anthropological perspectives on Israeli attitudes to the army and military service in order to enable readers to fully apprehend the values and visions the IDF symbolises in the eyes of the vast majority of Jewish-Israelis. Since the IDF is the one organisation endorsed by both Jewish-Israeli society and the state, he argues that portraits showing members of the family in military uniform operate as declarations of social assimilation and approval. However, while he demonstrates how such family photographs assist in perpetuating and solidifying Israeli social norms, including positive attitudes towards the Israeli army, he also shows how the protocols of the genre of portraiture–not least posing for the camera–can subvert the coherence of their putative message, thereby interfering with cultural constructions of martial identities tacitly endorsed by the state
6.3 A Sub-250mW 1-to-56Gb/s Continuous-Range PAM-4 42.5dB IL ADC/DAC-Based Transceiver in 7nm FinFET
No full textThe rapid spread of SARS-COV-2 Omicron variant in Italy reflected early through wastewater surveillance
No full textThe SARS-CoV-2 Omicron variant emerged in South Africa in November 2021, and has later been identified worldwide,
raising serious concerns.
A real-time RT-PCR assay was designed for the rapid screening of the Omicron variant, targeting characteristic mutations
of the spike gene. The assay was used to test 737 sewage samples collected throughout Italy (19/21 Regions) between
11 November and 25 December 2021, with the aim of assessing the spread of the Omicron variant in the
country. Positive samples were also tested with a real-time RT-PCR developed by the European Commission, Joint
Research Centre (JRC), and through nested RT-PCR followed by Sanger sequencing.
Overall, 115 samples tested positive for Omicron SARS-CoV-2 variant. The first occurrence was detected on 7
December, in Veneto, North Italy. Later on, the variant spread extremely fast in three weeks, with prevalence of positive
wastewater samples rising from 1.0% (1/104 samples) in the week 5–11 December, to 17.5% (25/143 samples)
in the week 12–18, to 65.9% (89/135 samples) in the week 19–25, in line with the increase in cases of infection with
the Omicron variant observed during December in Italy. Similarly, the number of Regions/Autonomous Provinces in
which the variant was detected increased fromone in the first week, to 11 in the second, and to 17 in the last one. The
presence of the Omicron variant was confirmed by the JRC real-time RT-PCR in 79.1% (91/115) of the positive samples,
and by Sanger sequencing in 66% (64/97) of PCR amplicons
AMPA receptor GluA2 subunit defects are a cause of neurodevelopmental disorders
No full textAMPA receptors (AMPARs) are tetrameric ligand-gated channels made up of combinations of GluA1-4 subunits encoded by GRIA1-4 genes. GluA2 has an especially important role because, following post-transcriptional editing at the Q607 site, it renders heteromultimeric AMPARs Ca2+-impermeable, with a linear relationship between current and trans-membrane voltage. Here, we report heterozygous de novo GRIA2 mutations in 28 unrelated patients with intellectual disability (ID) and neurodevelopmental abnormalities including autism spectrum disorder (ASD), Rett syndrome-like features, and seizures or developmental epileptic encephalopathy (DEE). In functional expression studies, mutations lead to a decrease in agonist-evoked current mediated by mutant subunits compared to wild-type channels. When GluA2 subunits are co-expressed with GluA1, most GRIA2 mutations cause a decreased current amplitude and some also affect voltage rectification. Our results show that de-novo variants in GRIA2 can cause neurodevelopmental disorders, complementing evidence that other genetic causes of ID, ASD and DEE also disrupt glutamatergic synaptic transmission. © 2019, The Author(s)
