1,720,966 research outputs found
Use of flow cytometry as a tool to study mitochondrial membrane potential in isolated, living hepatocytes
No full textThe present paper describes the possibility of determination of mitochondrial membrane potential (Delta omega) in isolated hepatocytes making use of a Delta psi-sensitive dye, i.e., the lipophilic cationic probe 5,5'6,6'-tetrachloro-1,1'3,3 '-tetraethylbenzimidazolcarbocyanine iodide (JC-1) and of cytofluorimetry. The validity of the method was proved by treating hepatocytes with FCCP (decrease of Delta psi) and subsequent addition of 6-ketocholestanol (increase of Delta psi). The results indicate that the proposed method may be used in laboratory practice
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Correzioni gravimetriche per la rete di livellazione geometrica italiana: stato dell’arte e l’esempio della Provincia di Trento
Full text linkRiassunto
Il sistema di riferimento altimetrico ufficiale italiano è definito attraverso una rete di livellazione geometrica materializzata e periodicamente rilevata dall’Istituto Geografico Militare (IGM).
Le quote dinamiche, ortometriche e normali dei capisaldi di una rete di livellazione si ottengono dalla compensazione ai minimi quadrati dei dislivelli geometrici osservati, opportunamente corretti per gli effetti di gravità. Il Politecnico di Milano (POLIMI), nell’ambito di una convenzione con l’IGM, sta effettuando il calcolo delle correzioni (dinamiche, ortometriche e normali) da apportare alle nuove osservazioni di livellazione eseguite nel contempo dall’IGM sull’intero territorio nazionale per l’aggiornamento del sistema di altezze italiano. I valori di gravità necessari al calcolo delle correzioni sono ricavati dal database utilizzato per il nuovo Geoide Italiano. Il Servizio Catasto della Provincia Autonoma di Trento in accordo con IGM e POLIMI partecipa all’acquisizione delle osservazioni della sotto-rete trentina e al successivo calcolo delle relative correzioni. Lo stato dell’arte nella definizione del sistema di riferimento delle altezze, con richiami sul calcolo delle correzioni gravimetriche classiche e su una nuova formulazione sviluppata di recente nell’ambito della teoria di Molodensky costituiscono l’oggetto della presente comunicazione insieme ai risultati preliminari ottenuti sui dati del Trentino.Abstract
The official italian height system is defined through a spirit leveling network periodically surveyed by the Istituto Geografico Militare (IGM). Dynamic, orthometric and normal heights of the network benchmarks are obtained form the least squares adjustment of the observed height differences, properly corrected for the gravity effects. In agreement with IGM, the Politecnico di Milano (POLIMI) is computing the corrections (dynamic, orthometric and normal) for the new spirit leveling observations, carried out in the meanwhile by the IGM to update the italian height datum. The gravity observations needed to evaluate the corrections are obtained from the same database used for the Italian Geoid. The Cadastral service of Provincia Autonoma di Trento in agreement with IGM and POLIMI is involved in the observation collection of the Trentino sub network as well as in the connected correction computation. The presentation is about the state of the art in the definition of the italian height datum and a short review on the gravity correction formulas as well as on a new formulation of the normal correction term in the framework of Molodensky’s theory. Preliminary results on the Trentino sub network will be presented as well
Decrease in mitochondrial energy couplings by thyroid hormones: a physiological effect rather than a pathological hyperthyroidism consequence
No full textThe effect of the in vivo thyroid status on mitochondrial membrane potential (Delta Psi(m)) in isolated rat hepatocytes was studies by means of a cytofluorimetric technique and the Delta Psi(m)-specific probe JC-1. It is shown that the Delta Psi(m) level decreases in the order hypothyroid > euthyroid > hyperthyroid, Polarographic measurement of the hepatocyte respiratory rates revealed an opposite trend of values: the highest respiratory rate in hepatocytes from hyperthyroid animals, the lowest in those from hypothyroid ones. This means that mitochondrial energy coupling is highest in hypothyroid hepatocytes and lowest in hyperthyroid hepatocytes. 6-Ketocholestanol added to hepatocytes failed to counterbalance the uncoupling effect of thyroid hormones on Delta Psi(m) and respiration rate. Under the same conditions, 6-ketocholestanol appeared to be effective in recoupling of respiration uncoupled by low concentrations of the artificial protonophore FCCP, The mechanism and possible physiological functions of the thyroid hormone-induced decrease in mitochondrial energy coupling are discussed. (C) 1998 Federation of European Biochemical Societies
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
- …
