6,682 research outputs found
O grande desafio da pesquisa.
No full textPraticamente dez anos após o surgimento da ferrugem asiática da soja, a agricultura brasileira enfrenta outra ameaça. Dessa vez, bem mais perigosa e devastadora. É a Helicoverpa armigera, surgida no oeste da Bahia durante a safra 2012/13, para depois espalhar-se para outras áreas de produção, como Mato Grosso, Distrito Federal e ParanáO diretor de Pesquisa e Desenvolvimento da entidade, Ladislau Martin Neto, fala sobre o que está sendo feito na busca do controle da Helicoverpa armigera. Uma das propostas é o Programa Nacional de Melhoramento Genético Preventivo.Entrevista
Caracterização da matéria orgânica de solos da região amazônica empregando a espectroscopia de fluorescência induzida por laser e a espectroscopia de emissão óptica com plasma induzido por laser.
No full textEntrada padronizada de BOAS, P. R. V.; MARTIN NETO, L
Distinção nutricional entre folhas de soja sadias e com sintoma de soja louca II utilizando a técnica espectroscopia de emissão óptica com plasma induzido por laser.
No full textEntrada padronizada: MARTIN-NETO, L
Centenarians, semi and supercentenarians, COVID-19 and Spanish flu: a serological assessment to gain insight into the resilience of older centenarians to COVID-19
No full textBackground: Although it is well known that the older people have been the most susceptible to COVID-19, there are conflicting data on the susceptibility of centenarians. Two epidemiological study have shown that older centenarians (> 101 years old at the time of the 2020 pandemic peak) are more resilient than the remaining centenarians, suggesting that this resilience might be linked to the 1918 Spanish Flu pandemic. To gain insight into this matter, specifically whether the resilience of older centenarians to SARS-CoV-2 infection is linked to the Spanish Flu they had been affected by, we conducted a retrospective serological study. This study examined serum samples from 33 centenarians, encompassing semi- (aged > 104 109 years, N = 4), born between 1905 and 1922, against both SARS-CoV-2 and 1918 H1N1 pseudotype virus.
Results: Anamnestic and laboratory data suggest that SARS-CoV-2 infection occurred in 8 centenarians. The infection appeared to have been asymptomatic or mild, and hospitalization was not required, despite 3 out of 8 being between 109 and 110 years old. The levels of anti-spike antibodies in centenarians infected and/or vaccinated were higher, although not significantly, than those produced by a random sample of seventy-year-old individuals used as controls. All centenarians had antibody levels against the 1918 H1N1 virus significantly higher (almost 50 times) than those observed in the quoted group of seventy-year-old subjects, confirming the key role in maintaining immunological memory from a priming that occurred over 100 years ago. Centenarians whose blood was collected prior to the pandemic outbreak demonstrated neutralising antibodies against the 1918 H1N1 virus, but all these subjects tested negative for SARS-CoV-2.
Conclusion: This retrospective study shows that older centenarians are quite resilient to COVID-19, as they are capable of producing good levels of neutralising antibodies and experiencing mild or asymptomatic disease. This could be attributed to the 1918 Spanish flu pandemic through mechanisms other than the presence of cross-reactive antibodies between the 1918 H1N1 virus and SARS-CoV-2. Another possibility is that the association is purely temporal, solely correlated with the advanced age of resilient centenarians compared to those born after 1918, since older centenarians are known to have better control of immune-inflammatory responses
Development and optimisation of influenza C and influenza D pseudotyped viruses
No full textTo facilitate the study of influenza C (ICV) and influenza D (IDV) viruses, we generated lentiviral pseudotyped viruses (PVs) expressing the hemagglutinin-esterase fusion (HEF) glycoprotein from ICV (C/Minnesota/33/2015) and IDV (D/Swine/Italy/199724-3/2015, D/Bovine/France/5920/2014, and D/Bovine/Ibaraki/7768/2016). The production of these PVs was optimised using different amount of human airway trypsin-like (HAT) protease to enhance HEF maturation, and the transduction efficiency was evaluated in multiple cell lines. Using these PVs, we established a pseudovirus-based microneutralisation (pMN) assay to measure neutralising antibody responses and adapted an esterase activity assay to evaluate PV. Specific antisera neutralised PVs but failed to inhibit esterase activity. These findings confirm that ICV and IDV PVs provide a scalable, sensitive, and safe tool for antiviral screening, and sero-epidemiological research
Synthesis and evaluation of potential cholinesterases inhibitors
No full textUniverzita Karlova, Farmaceutická fakulta v Hradci Králové Katedra organické a bioorganické chemie Řešitel práce: Neto-Honorius Houngbedji Vedoucí práce: PharmDr. Mgr. Martin Krátký, Ph.D. Název diplomové práce: Syntéza a hodnocení potenciálních inhibitorů cholinesteráz Počet pacientů trpících Alzheimerovou chorobou v posledních letech neustále narůstá. Tato choroba, zatěžující jak pacienta, tak jeho okolí, vyžaduje nákladnou léčbu, která t.č. spočívá především v používání inhibitorů acetylcholinesterázy. Teoretická část práce shrnuje aktuální poznatky o patologii, epidemiologii a terapii Alzheimerovy choroby. Prezentuje také výsledky předešlého výzkumu v oblasti salicylanilidových inhibitorů cholinesteráz. Používání inhibitorů acetylcholinesterázy v terapii Alzheimerovy choroby vedlo k navržení osmi nových látek na bázi esterů kyselin fosforu a salicylanilidů s inhibiční aktivitou vůči cholinesterázám. Náplň praktické části diplomové práce tak spočívá v syntéze, charakterizaci a biologickém hodnocení nových inhibitorů cholinesteráz. Ve výsledku se podařilo syntetizovat a vyhodnotit tři nové sloučeniny, jejichž inhibiční aktivita (IC50) se pohybovala v rozmezí 48,13-66,13 µM vůči acetylcholinesteráze a 2,37-170,10 µM vůči butyrylcholinesteráze. U nejaktivnějšího inhibitoru...Charles University, Faculty of Pharmacy in Hradec Králové Department of Organic and Bioorganic Chemistry Author: Neto-Honorius Houngbedji Supervisor: PharmDr. Mgr. Martin Krátký, Ph.D. Title of diploma thesis: Synthesis and evaluation of potential inhibitors of cholinesterases During the last years, the number of patients affected by Alzheimer's disease has been constantly increasing. This disease, which burdens the patients as well as their milieu, requires a costly treatment, based on the use of acetylcholinesterase inhibitors currently. The theoretical part summarizes the contemporary knowledge about the pathology, epidemiology and therapy of Alzheimer's disease. This part also presents the results of previous research of salicylanilide-based cholinesterase inhibitors. The use of acetylcholinesterase inhibitors in the treatment of Alzheimer's disease led to the design of eight novel compounds (esters of salicylanilides and phosphorus acids) with inhibitory activity against cholinesterases. Thus, the experimental part of this diploma thesis consists in the synthesis, characterization and biological evaluation of the novel inhibitors. Finally, three new compounds were synthesized and evaluated successfully. Their inhibitory activities (IC50) were within the range of 48.13-66.13 µM against...Katedra organické a bioorganické chemieDepartment of Organic And Bioorganic ChemistryFaculty of Pharmacy in Hradec KrálovéFarmaceutická fakulta v Hradci Králov
“Psicologia da composição” – aproximação do pensamento de João Cabral de Melo Neto
No full textThis paper aims to analyze the poem "Psychology of Composition" by João Cabral de Melo Neto, seeking to comprehend poetry and poetic creation through the phenomenon of language as understood by Martin Heidegger.Trata-se de analisar o poema “Psicologia da composição” de João Cabral de Melo Neto visando compreender a poesia e o fazer poético a partir do fenômeno da linguagem, tal como Martin Heidegger o compreende
Synthesis and evaluation of potential cholinesterases inhibitors
No full textCharles University, Faculty of Pharmacy in Hradec Králové Department of Organic and Bioorganic Chemistry Author: Neto-Honorius Houngbedji Supervisor: PharmDr. Mgr. Martin Krátký, Ph.D. Title of diploma thesis: Synthesis and evaluation of potential inhibitors of cholinesterases During the last years, the number of patients affected by Alzheimer's disease has been constantly increasing. This disease, which burdens the patients as well as their milieu, requires a costly treatment, based on the use of acetylcholinesterase inhibitors currently. The theoretical part summarizes the contemporary knowledge about the pathology, epidemiology and therapy of Alzheimer's disease. This part also presents the results of previous research of salicylanilide-based cholinesterase inhibitors. The use of acetylcholinesterase inhibitors in the treatment of Alzheimer's disease led to the design of eight novel compounds (esters of salicylanilides and phosphorus acids) with inhibitory activity against cholinesterases. Thus, the experimental part of this diploma thesis consists in the synthesis, characterization and biological evaluation of the novel inhibitors. Finally, three new compounds were synthesized and evaluated successfully. Their inhibitory activities (IC50) were within the range of 48.13-66.13 µM against..
Sequestro de carbono no solo por braquiária: importância para a produção pecuária sustentável.
No full text[Conto] Diáspora
No full textA short story by Angolan writer Carmo Neto, published under the author\u27s permissionCuento del escritor angoleño Carmo Neto, aquí publicado con su autorización.Conto do escritor angolano Carmo Neto, aqui publicado com sua autorização
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