130,558 research outputs found
Marrano set
No full textMarrano set. (a) Brass kiddush cup Kol Bo with leaf pattern and semi-precious stones. Handles at sides. Removable lid and finial. Finial serves as spice container (e). Inside are 2 smaller cups (b & c). Larger one is inscribed in Hebrew; inside smaller cup is miniature scroll of Esther (f) hand written on parchment in case. Beneath base stand is hanukkah lamp for oil, inscribed with temple menorah and rampant lions.(d)Digital imagedigitize
Enucleazioni ed enucleoresezioni delle lesioni dell’istmo e del corpo-coda del pancreas.
No full text
Genomic signatures of different adaptations to environmental stimuli between wild and cultivated Vitis vinifera L.
No full textThe application of population genetic methods in combination with gene mapping strategies can help to identify
genes and mutations selected during the evolution from wild plants to crops and to explore the considerable genetic
variation still maintained in natural populations. We genotyped a grapevine germplasm collection of 44 wild (Vitis
vinifera subsp. sylvestris) and 48 cultivated (V. vinifera subsp. sativa) accessions at 54 K single-nucleotide polymorphisms
(SNPs) to perform a whole-genome comparison of the main population genetic statistics. The analysis of Wright
Fixation Index (FST) along the whole genome allowed us to identify several putative “signatures of selection” spanning
over two thousand SNPs significantly differentiated between sativa and sylvestris. Many of these genomic regions
included genes involved in the adaptation to environmental changes. An overall reduction of nucleotide diversity was
observed across the whole genome within sylvestris, supporting a small effective population size of the wild grapevine.
Tajima’s D resulted positive in both wild and cultivated subgroups, which may indicate an ongoing balancing
selection. Association mapping for six domestication-related traits was performed in combination with population
genetics, providing further evidence of different perception and response to environmental stresses between sativa
and sylvestris
\"La inquisición y el labirinto marrano\": cultura, poder y repression na Galiza (sécs. XVI e XVII)\"
No full textO objetivo desta dissertação consiste em demonstrar a existência de um \"labirinto\" na condição do \"ser marrano\" que está atrelado a uma situação de perseguição que se comprova pela comparação entre as causas despachadas nas visitações do Santo Ofício, e as causas efetivamente julgadas ou continuadas no âmbito do tribunal. Este labirinto é constituido por duas faces. Uma, objetiva, forma-se através das acusações pelo crime de judaísmo, pelos critérios de julgamento iterpostos na situação de julgamento, no ato de inquirição, no caso da perseguição nas visitações ou do julgamento no tribunal. Outra, subjetiva, reside na experiência de uma condição de divisão do próprio ego do marrano. Como decorrência desta perseguição, esta divisão atinge as estruturas sociais que lhe acessoram, modificando instâncias tais como a família e o grupo marrano em suas diversas configurações.El objectivo de este trabajo consiste en demostrar la existencia de un \"labirinto\" en la condición del \"ser\" marrano ligado a una situación de persecución que se comprueba por la comparación entre las causas despachadas en las visitas del Santo Oficio y las causas efectivamente juzgadas o continuadas en el ámbito del tribunal. Este labirinto es constituido por dos anversos. Uno, objectivo, se forma través de las acusaciones por el crimen de judaísmo, por los criterios de juzgamiento iterpuestos en una situación de juzgamiento, en el acto de inquirición, en el caso de la persecución en las visitas o del juzgamiento en el tribunal. Otra, subjectiva, reside en la experiencia de una condición de división del propio ego del marrano. En decorrencia de esta persecución, esta división atinge las estructuras sociales que le acesoran, modificando instancias tales como la familia y el grupo marrano en sus diversas configuraciones
PHOSPHO-REGULATION OF ACA8, A PLASMA MEMBRANE CA2+-ATPASE OF ARABIDOPSIS THALIANA
No full textACA8 is a plasma membrane-localized isoform of calmodulin (CaM)-regulated Ca2+-ATPase of Arabidopsis thaliana. Phospho-proteomic studies identified several phosphopeptides corresponding to portions of its regulatory N-terminus. Each of the Ser found to be phosphorylated in those studies (S19, S22, S27, S29, S57, and S99) has been mutated to Asp, to mimic phosphorylation of the ACA8 N-terminus, and to Ala to prevent phosphorylation. Mutants have been expressed in Saccharomyces cerevisiae and characterized: as shown by the low activation by CaM, mutants S19D, S57D, S22D and S27D are deregulated. Moreover, the low response to CaM of ACA8 mutants S22A, S27A, and S29A points the relevance of these serine residues per se in determining the amplitude of the response of ACA8 to CaM. To analyse the effect of S to D mutation on the kinetic of CaM binding, His-tagged N-termini of wild-type and mutant ACA8 (6His-1M-I116) were expressed in Escherichia coli, affinity-purified and used in surface plasmon resonance experiments. All the analysed mutations affect the kinetics of interaction with CaM to some extent: in most cases, the altered kinetics result in marginal changes in affinity, with the exception of mutants S57D (KD 10-fold higher than wild-type ACA8) and S99D (KD about half that of wild-type ACA8). Since S19 is in a consensus motive for calcium-dependent protein kinases (CDPKs) the ACA8 N-terminus has been subjected to in vitro phosphorylation assays with two isoforms of A. thaliana CDPKs: CDPK1, that phosphorylates ACA2 (an endoplasmic reticulum localised isoform of A. thaliana ACA) and CDPK16, a plasma membrane localised isoform of CDPK. Results show that both kinases are able to phosphorylate ACA8 N-terminus, but CDPK16 with higher extent. Phosphorylation of mutant 6His-1M-I116 peptides mapped CDPK16 phosphorylation site at S19 and at S22. Furthermore, we identified by two-hybrid screening two isoforms of CBL-interacting protein kinases (CIPKs) as putative interactors of ACA8 N-terminus region: CIPK9 and CIPK14. BiFC analysis in Nicotiana benthamiana confirmed the two-hybrid results, showing that interaction between ACA8 full length and CIPK9 or CIPK14 occurs in planta at the plasma membrane. Moreover, phosphorylation assay demonstrate that both kinases phosphorylate ACA8 N-terminus in vitro. Implications of these results are discussed
- …
