3,694 research outputs found
The intracellular antibody capture technology (IACT): Towards a consensus sequence for intracellular antibodies
No full textWe describe the application of an intracellular antibody capture technology (IACT) as a generic in vivo selection procedure for isolating intracellular antibodies or ICAbs. IACT was applied to the de novo selection of functional ICAbs against the microtubule-associated protein TAU, found in neurofibrillary lesions of Alzheimer's disease brains. A panel of 17 different ICAbs was created which bind TAU inside cells and the epitopes recognized by the selected ICAbs have been determined by an in vivo epitope mapping procedure. Finally, sequence analysis showed that the IACT-derived ICAbs are characterized by a common signature of conserved amino acid residues, suggesting that the IACT naturally selects a sort of "captured consensus sequence" for intracellular antibodies. The development of IACT, together with the possibility of scaling up in a high throughput and automated format, makes IACT a new enabling tool for target validation in functional genomics and global proteomics
Cardozo Marks New School Year by Welcoming Newest Class of JD, LL.M. Students
No full textCardozo welcomed its newest members of the JD and LL.M. community during orientation this week.
Approximately 260 students in the fall JD class began their law school journey. Many are native New Yorkers, while others traveled from other countries to study at Cardozo. About half have already worked in the legal field with various law firms or in different professional careers.https://larc.cardozo.yu.edu/cardozo-news-2024/1029/thumbnail.jp
Cardozo Marks New School Year by Welcoming Newest Class of JD, LL.M. Students
Full text linkCardozo welcomed its newest members of the JD and LL.M. community during orientation this week.
Approximately 260 students in the fall JD class began their law school journey. Many are native New Yorkers, while others traveled from other countries to study at Cardozo. About half have already worked in the legal field with various law firms or in different professional careers.https://larc.cardozo.yu.edu/cardozo-news-2024/1029/thumbnail.jp
Predicting antigenic peptides suitable for the selection of phage antibodies
No full textSelection from phage antibody libraries can be considered to be an in vitro immune system in which the antibody response is reduced to the bare minimum of antigen recognition. Using selections of antibodies on peptides from a phage antibody library, we investigated what constitutes peptide antigenicity in the context of the antibody-protein binding site. We selected polyclonal antibodies in a high throughput format against 44% of 90 overlapping peptides derived from three different proteins. Of these, 33% of peptides (epitopic peptides) were able to select antibodies that recognized the protein from which the peptides were derived. Although no algorithm was able to predict all epitopic peptides, solvent accessibility was the best predictor in this cell-free antibody selection context. We subsequently applied solvent accessibility to successfully predict epitopic peptides from p53 and Znf217, and showed that such peptide selected single-chain antibodies were able to recognize soluble p53 in ELISA and Znf217 in a western blot. This is likely to have considerable utility in functional genomics and proteomics where it should be possible to select antibodies against gene products on the basis of deduced amino acid sequence in a high throughput fashion
Characterization of a single-chain intrabody directed against the human receptor tyrosine kinase Ron
No full textConsideration of Interference Correlation Properties in a JD-CDMA Mobile Radio System with Coherent Receiver Antenna Diversity
No full textIn code division multiple access (CDMA) mobile radio systems, both intersymbol interference and multiple access interference arise which can be combatted by using Joint Detection (JD) techniques, to reduce the degradation in performance resulting from time variance, coherent receiver antenna diversity (CRAD) can be used. The application of JD techniques offers the possibility to exploit the knowledge of noise covariances at the receiver. If only intercell (cochannel) interference is considered, the noise covariances in the uplink receiver of a multiple receiver antenna CDMA mobile radio system depend mainly on the directions of arrival (DOAs) of the interfering signals and the receiver antenna placement. Therefore, if the interferer DOAs are known at the base station, these covariances could be estimated. In this thesis, a realistic model of the uplink of a JD CDMA mobile radio system with CRAD is described in which the above mentioned interference cancelling method is used. Simulation results according to this model are given and evaluated.Applied SciencesElectrical EngineeringTelecommunications and Traffic Control Systems Grou
Dairy farmers’ perceptions toward the implementation of on-farm Johne’s disease prevention and control strategies
No full textmplementation of specific management strategies on dairy farms is currently the most effective way to reduce the prevalence of Johne’s disease (JD), an infectious chronic enteritis of ruminants caused by Mycobacterium avium subspecies paratuberculosis (MAP). However, dairy farmers often fail to implement recommended strategies. The objective of this study was to assess perceptions of farmers participating in a JD prevention and control program toward recommended practices, and explore factors that influence whether or not a farmer adopts risk-reducing measures for MAP transmission. Semi-structured interviews were conducted with 25 dairy farmers enrolled in a voluntary JD control program in Alberta, Canada. Principles of classical grounded theory were used for participant selection, interviewing, and data analysis. Additionally, demographic data and MAP infection status were collected and analyzed using quantitative questionnaires and the JD control program database. Farmers’ perceptions were distinguished according to 2 main categories: first, their belief in the importance of JD, and second, their belief in recommended JD prevention and control strategies. Based on these categories, farmers were classified into 4 groups: proactivists, disillusionists, deniers, and unconcerned. The first 2 groups believed in the importance of JD, and proactivists and unconcerned believed in proposed JD prevention and control measures. Groups that regarded JD as important had better knowledge about best strategies to reduce MAP transmission and had more JD risk assessments conducted on their farm. Although not quantified, it also appeared that these groups had more JD prevention and control practices in place. However, often JD was not perceived as a problem in the herd and generally farmers did not regard JD control as a “hot topic” in communications with their herd veterinarian and other farmers. Recommendations regarding how to communicate with farmers and motivate various groups of farmers according to their specific perceptions were provided to optimize adoption of JD prevention and control measures and thereby increase success of voluntary JD control programs
Knowledge gaps that hamper prevention and control of Mycobacterium avium subspecies paratuberculosis infection
No full textIn the last decades, many regional and country‐wide control programmes for Johne's disease (JD ) were developed due to associated economic losses, or because of a possible association with Crohn's disease. These control programmes were often not successful, partly because management protocols were not followed, including the introduction of infected replacement cattle, because tests to identify infected animals were unreliable, and uptake by farmers was not high enough because of a perceived low return on investment. In the absence of a cure or effective commercial vaccines, control of JD is currently primarily based on herd management strategies to avoid infection of cattle and restrict within‐farm and farm‐to‐farm transmission. Although JD control programmes have been implemented in most developed countries, lessons learned from JD prevention and control programmes are underreported. Also, JD control programmes are typically evaluated in a limited number of herds and the duration of the study is less than 5 year, making it difficult to adequately assess the efficacy of control programmes. In this manuscript, we identify the most important gaps in knowledge hampering JD prevention and control programmes, including vaccination and diagnostics. Secondly, we discuss directions that research should take to address those knowledge gaps
Electronic structure of phosphorus and arsenic δ-doped germanium
No full textDensity functional theory in the LDA+U approximation is used to calculate the electronic structure
of germanium δ-doped with phosphorus and arsenic. We characterize the principal band minima of the two-dimensional electron gas created by δ-doping and their dependence on the dopant concentration. Populated first at low concentrations is a set of band minima at the perpendicular projection of the bulk conduction band minima at L into the (kx,ky) plane. At higher concentrations band minima at Γ and Δ become involved. Valley splittings and effective masses are computed using an explicit-atom approach, taking into account the effects of disorder in the arrangement of dopant atoms in the δ-plane
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