27 research outputs found
Lipid-modified 24 NT DNA
No full textHi-resolution images from my publications
generated by pymol without computation
Carrier-free micellar CpG interacting with cell membrane for enhanced immunological treatment of HIV-1
Haejoo Kim, Wei Zhang, Juyoung Hwang, Eun-Koung An, Yeol Kyo Choi, Eunyoung Moon, Mark Loznik, Yang Hoon Huh, Andreas Herrmann, Minseok Kwak*, Jun-O Jin*
Biomaterials 2021, 277, 121081.
10.1016/j.biomaterials.2021.121081</p
Kontrolle über die optische und katalytische Aktivität gentechnisch hergestellter Proteine mit Ultraschall
No full textAuthor Correction : Mechanochemical bond scission for the activation of drugs
No full textA Correction to this paper has been published: https://doi.org/10.1038/s41557-021-00685-3
Modular and Versatile Trans‐Encoded Genetic Switches
No full textCurrent bacterial RNA switches suffer from lack of versatile inputs and are difficult to engineer. We present versatile and modular RNA switches that are trans-encoded and based on tRNA-mimicking structures (TMSs). These switches provide a high degree of freedom for reengineering and can thus be designed to accept a wide range of inputs, including RNA, small molecules, and proteins. This powerful approach enables control of the translation of protein expression from plasmid and genome DNA.</p
Accelerating chemical reactions by molecular sledding
No full textThe speed-up of covalent bond formation was achieved between a sulfhydryl group and a 2-bromopropionic acid derivative by utilizing sliding peptide-modified substrates. Moreover, a new type of DNA cleaving reagent was developed, consisting of pVIc covalently coupled to verteporfin. This peptide-porphyrin conjugate allowed targeting of DNA and resulted in increased photodegradation of double-stranded nucleic acids.</p
Controlling Optical and Catalytic Activity of Genetically Engineered Proteins by Ultrasound
No full textUltrasound (US) produces cavitation-induced mechanical forces stretching and breaking polymer chains in solution. This type of polymer mechanochemistry is widely used for synthetic polymers, but not biomacromolecules, even though US is biocompatible and commonly used for medical therapy as well as in vivo imaging. The ability to control protein activity by US would thus be a major stepping-stone for these disciplines. Here, we provide the first examples of selective protein activation and deactivation by means of US. Using GFP as a model system, we engineer US sensitivity into proteins by design. The incorporation of long and highly charged domains enables the efficient transfer of force to the protein structure. We then use this principle to activate the catalytic activity of trypsin by inducing the release of its inhibitor. We expect that this concept to switch “on” and “off” protein activity by US will serve as a blueprint to remotely control other bioactive molecules. © 2020 The Authors. Angewandte Chemie International Edition published by Wiley-VCH Gmb
DNA hybridization as a general method to enhance the cellular uptake of nanostructures
No full textThe biomedical application of nanoparticles (NPs) for diagnosis and therapy is considerably stalled by their inefficient cellular internalization. Many strategies to overcome this obstacle have been developed but are not generally applicable to different NP systems, consequently underlining the need for a universal method that enhances NP entry into cells. Here we describe a method to increase NP cellular uptake via strand hybridization between DNA-functionalized NPs and cells that bear the respective complementary sequence incorporated into the membrane. By this, the NPs bind efficiently to the cellular surface enhancing internalization of three completely different NP types: DNA tetrahedrons, gold (Au) NPs, and polystyrene (PS) NPs. We show that our approach is a simple and generalizable strategy that can be applied to virtually every functionalizable NP system
Electrostatically PEGylated DNA enables salt-free hybridization in water
No full textChemically modified nucleic acids have long served as a very important class of bio-hybrid structures. In particular, the modification with PEG has advanced the scope and performance of oligonucleotides in materials science, catalysis and therapeutics. Most of the applications involving pristine or modified DNA rely on the potential of DNA to form a double-stranded structure. However, a substantial requirement for metal-cations to achieve hybridization has restricted the range of applications. To extend the applicability of DNA in salt-free or low ionic strength aqueous medium, we introduce noncovalent DNA-PEG constructs that allow canonical base-pairing between individually PEGylated complementary strands resulting in a double-stranded structure in salt-free aqueous medium. This method relies on grafting of amino-terminated PEG polymers electrostatically onto the backbone of DNA, which results in the formation of a PEG-envelope. The specific charge interaction of PEG molecules with DNA, absolute absence of metal ions within the PEGylated DNA molecules and formation of a double helix that is significantly more stable than the duplex in an ionic buffer have been unequivocally demonstrated using multiple independent characterization techniques.</p
