410 research outputs found
Carta sismica d'Italia : aree di scuotimento
No full textconstruito secondo gli studi di Mario Baratta[in Mappe
Mario Baratta. Il Vesuvio e le sue Eruzioni
No full textChaix Émile. Mario Baratta. Il Vesuvio e le sue Eruzioni. In: Le Globe. Revue genevoise de géographie, tome 36, 1897. p. 189
Mrs Mario Baratta et Luigi Visintin, Atlante delle Colonie Italiane
No full textFebvre Lucien. Mrs Mario Baratta et Luigi Visintin, Atlante delle Colonie Italiane. In: Annales d'histoire économique et sociale. 2ᵉ année, N. 7, 1930. p. 433
Methods in Molecular Biology
No full textSkeletal muscle in cattle occupies a large part of the animal’s body mass and develops into an important source of nutrients for human nutrition. Recently, the attention on bovine myogenic cells is increased to develop strategies of cultured in vitro meat as an alternative food source, more sustainable, ethical, and healthy than traditional meat production. At present, investigating the proliferation and differentiation of bovine skeletal muscle myogenic cells in vitro maintains its importance in the study of the mechanisms underlying the physiological and pathological events affecting the skeletal muscle, but it is of particular interest in animal husbandry and the food industry fields.
In cell-based biological research, cell lines are one of the favored experimental tools because a population of cells could proliferate indefinitely in vitro under different stimuli, but they are limited to addressing the relevant biological properties of a cell population. On the other hand, primary cells from normal animal tissues undergo a limited number of divisions in vitro before they enter senescence but preserve their original characteristics and functions, and researchers can acquire the opportunity to study the individual donors and not just cells.
In this chapter, we provide a basic protocol to isolate satellite cells from the skeletal muscle of cattle to obtain a good number of myogenic cells that can grow in in vitro conditions and undergo multiple rounds of cell division (myoblasts) before entering differentiation (myotubes). Furthermore, the robust expansion of these cells leads to the possibility to investigate physiological events or disorders related to the skeletal muscle tissue
Epithelial Cell Culture 2nd Edition
No full textEpithelial cell culture has emerged as a cornerstone technique in the field of cell biology,
providing invaluable insights into the fundamental mechanisms underlying various physiological
processes. It is with great pleasure and excitement that we present the second edition
of Epithelial Cell Culture: Methods and Protocols, which builds upon the success of the first
edition and further delves into the dynamic world of epithelial cell researc
Mrs Mario Baratta et Luigi Visintin, L'Atlante della Produzione e dei Commerci
No full textFebvre Lucien. Mrs Mario Baratta et Luigi Visintin, L'Atlante della Produzione e dei Commerci. In: Annales d'histoire économique et sociale. 2ᵉ année, N. 7, 1930. pp. 432-433
Role of Leptin in the Mammary Gland Development, Lactation and in Neonatal Physiology
No full textAbstract:
The biology of leptin has been studied most extensively in the central nervous system for the regulation of food intake and energy balance. In recent years, a growing number of publications have reported several activities of this adipose-secreted protein in different organs. These effects appear to be independent of the regulation of food intake or at least not directly correlated to it, but rather related to the hormonal regulation of these particular tissues. Thus leptin is now also considered to be a hormonal factor that informs several hormonal circuits and biological peripheral functions of the nutrition status of the organism. Evidences are reported the role of leptin to regulate mammogenesis during a virgin, pregnancy and involution. In mammary gland, leptin has been observed to exert also an autocrine and/or paracrine activity which affects the development of duct, formation of gland alveolus, expression of milk protein gene and onset involution of mammary gland. Findings with experimental rodent models reveal that exposures to leptin during the in utero and pubertal periods when the mammary gland is undergoing extensive modeling and re-modeling, may alter susceptibility to develop mammary tumors. Leptin synthesis has been found also in the placenta both in human and in livestock animals suggesting a role in controlling growth of the foetus and neonate. Furthermore, colostrum and milk contain high amounts of leptin, in particular during the first few days of lactation, that cause a correlation between milk leptin and plasma leptin, body weight and body mass index. Furthermore, other studies suggest that milk leptin may control appetite. Lastly, since nutrition or neonatal stress can program the immune system, leptin change that occurs in mothers and neonates can imprint hormonal or metabolic changes that influence later life degenerative and chronic diseases
Bovine Mammary Organoids: A Model to Study Epithelial Mammary Cells
No full textBovine mammary organoids are cell aggregates that are produced by an association of a mechanical and an enzymatic dissociation of mammary gland tissue. They provide a useful source to isolate mammary epithelial cells, but can also be frozen as an intermediate dissociation step. Due to strong cell-cell interactions among epithelial cells, the production and isolation of organoids is an efficient way to remove unwanted cell population of non-epithelial origin like fibroblasts
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