82 research outputs found
Conclusions: Alberto Garlandini et Bruno Brulon en conversation avec des étudiants en muséologie et gens de musée
No full text"In the closing session, the Babel Tower discussed preliminary reflections and posed some questions both to Alberto Garlandini, and Bruno Brolon Soares. They have been invited to share their thoughts regarding a possible fil rouge of the Babel Tower, in relation with the debate that is emerging inside ICOM and ICOFOM. How can the issues discussed during the webinars contribute to the broad debate related with a post-pandemic world? What’s the role of the types of museums and practices presented during the series – such as ecomuseums – in this historical phase? What important pieces are missing, but should be incorporated in order to enrich the picture presented in the Babel Tower?" Giusy PappalardoLes muséologies insurgées: échanges transnationaux11. Sustainable cities and communities10. Reduced inequalitie
Development of novel biomarkers of stress: alterations of cell signaling induced by organic and inorganic pollutants
No full textCyclic fatigue resistance, torsional resistance, and metallurgical characteristics of M3 Rotary and M3 Pro Gold NiTi files
No full textObjectives: To evaluate the mechanical properties and metallurgical characteristics of the M3 Rotary and M3 Pro Gold files (United Dental).
Materials and Methods: One hundred and sixty new M3 Rotary and M3 Pro Gold files (sizes 20/0.04 and 25/0.04) were used. Torque and angle of rotation at failure (n = 20) were measured according to ISO 3630-1. Cyclic fatigue resistance was tested by measuring the number of cycles to failure in an artificial stainless steel canal (60° angle of curvature and a 5-mm radius). The metallurgical characteristics were investigated by differential scanning calorimetry. Data were analyzed using analysis of variance and the Student-Newman-Keuls test.
Results: Comparing the same size of the 2 different instruments, cyclic fatigue resistance was significantly higher in the M3 Pro Gold files than in the M3 Rotary files (p < 0.001). No significant difference was observed between the files in the maximum torque load, while a significantly higher angular rotation to fracture was observed for M3 Pro Gold (p < 0.05). In the DSC analysis, the M3 Pro Gold files showed one prominent peak on the heating curve and 2 prominent peaks on the cooling curve. In contrast, the M3 Rotary files showed 1 small peak on the heating curve and 1 small peak on the cooling curve.
Conclusions: The M3 Pro Gold files showed greater flexibility and angular rotation than the M3 Rotary files, without decrement of their torque resistance. The superior flexibility of M3 Pro Gold files can be attributed to their martensite phase
MALAT1 Expression Is Deregulated in miR-34a Knockout Cell Lines
No full textBackground/Objectives: Non-coding microRNA-34a (miR-34a) regulates the expression of key factors involved in several cellular processes, such as differentiation, apoptosis, proliferation, cell cycle, and senescence. Deregulation of the expression of these factors is implicated in the onset and progression of several human diseases, including cancer, neurodegenerative disorders, and pathologies associated with viral infections and inflammation. Despite numerous studies, the molecular mechanisms regulated by miR-34a remain to be fully understood. The present study aimed to generate miR-34a knockout cell lines to identify novel genes potentially regulated by its expression. Methods: We employed the CRISPR-Cas9 gene editing system to knock out the hsa-miR-34a gene in HeLa and 293T cell lines, two widely used models for studying molecular and cellular mechanisms. We compared proliferation rates and gene expression profiles via RNA-seq and qPCR analyses between the wild-type and miR-34a KO cell lines. Results: Knockout of miR-34a resulted in a decreased proliferation rate in both cell lines. Noteworthy, the ablation of miR-34a resulted in increased expression of the long non-coding RNA MALAT1. Additionally, miR-34a-5p silencing in the A375 melanoma cell line led to MALAT1 overexpression. Conclusions: Our findings support the role of the miR-34a/MALAT1 axis in regulating proliferation processes
Shelf-life extension of leavened bakery products by using bio-protective cultures and type-III sourdough
No full textPhysicochemical and microbiological alteration of processed foods limits their shelf-life. Spoiled goods are on the watch list of industries interested to identify innovative strategies allowing for shelf-life extension and avoiding changes of safety and organoleptic features, being involved in food waste and economic loss matters. To extend the shelf-life of two different types of bakery products, we evaluated the effectiveness of a type-III sourdough (tIII-SD) combined with a mixture of probiotics (Lactobacillus acidophilus, L. casei, Bifidobacterium sp., Bacillus coagulans) that were used as bioprotective-cultures (BCs). This innovative (I) dough was used to produce fresh base- pizza (BP) and focaccia (FO) that were inspected by a multi-omics approach aimful at monitoring features at different time-points of the shelf-life. Differences in physicochemical, protein, microbiological and volatile profiles were also investigated after 10-days of extended shelf-life. The addition of BCs and tIII-SD left unchanged the proximate composition. This, together with the absence of detected microbial contaminations, indicated the suitability of both I-BP and I- FO despite the shelf-life extension. Both I-samples accounted for a more stable and heterogeneous microbiota during the storage phase and showed lower scores of Alternaria infectoria and A. alternata. In I- samples, volatilomics showed an increased relative concentration of volatile carboxylic acids. Therefore, without resorting to chemical preservatives, the addition of BCs and tIII-SD led to specific microbiological and metabolite improvements in both BP and FO products, whose shelf-life was extended by 10-days under MAP. Shelf life extension can play a key role for reducing food waste and is agreed as an effective way to increase the sustainability of food systems contributing to achieve both the Sustainable Development Goals and the goals of the Paris Agreement on climate change
Correction to: Safety and efficacy of COVID-19 vaccines in patients on dialysis: a multicentre cohort study in Italy
No full textThe COVIDVaxDia Study Group in html which currently reads Francesca Menniti-Ippolito, Roberto Da Cas, Flavia Chiarotti, Massimo Fabiani, Giuseppe Traversa, Piergiorgio Messa, Alfonso Mele, Salvatore De Masi, Francesca Colavita, Concetta Castilletti, Carmine Zoccali, Aldo Pietro Maggioni, Andrea Lorimer, Martina Ceseri, Ester Baldini, Francesca Bianchini, Laura Sarti, Giovanni Baglio, Andrea Mariano and Eva Alessi. It should read as: Francesca Menniti-Ippolito, Roberto Da Cas, Flavia Chiarotti, Massimo Fabiani, Giuseppe Traversa, Piergiorgio Messa, Alfonso Mele, Salvatore De Masi, Francesca Colavita, Concetta Castilletti, Carmine Zoccali, Aldo Pietro Maggioni, Andrea Lorimer, Martina Ceseri, Ester Baldini, Francesca Bianchini, Laura Sarti, Giovanni Baglio, Andrea Mariano & Eva Alessi, Lorenzo Di Liberato, Luigi Amoroso, Nicola Spetrino, Milva Di Giovanni, Carlo Sapio, Irma Figlia, Roberto Pititto, Teresa Papalia, Rosita Greco, Francesca Leone, Francesco Maria D’Agostino, Mariangela Campolo, Maria Rosa La Gamba, Francesca Mallamaci, Vincenzo Panuccio, Giovanna Parlongo, Giuseppe Natale, Domenico Tramontana, Ivania Maria Figliano, Santo Vitiello, Anna Maria Frangiosa, Domenico Caserta, Pasqualina Acconcia, Nunzia Paudice, Alfonso De Maio, Salvatore Cascone, Marilina Siani, Mario Cioffi, Nataliya Romanyuk, Franca Pagnano, Antonio De Donato, Ersilia Satta, Alessandra Perna, Francesco Trepiccione, Alessandro Cerrone, Gianluca Garofalo, Giancarlo Marinelli, Roberta D’Amato, Enrico De Felice, Alfredo Vacca, Salvatore Coppola, Vincenzo Cuomo, Maria Palma Iavarone, Annalisa Ciotola, Vincenzo Puglia, Pio Granato, Carla Lamberti, Giorgio Capasso, Domenico Bonanno, Marco Ventre, Veronica Amendola, Fabio Cappabianca, Elena Mancini, Davide Ricci, Daniela Cecilia Cannarile, Roberta Benevento, Bianca Perciaccante, Alda Storari, Yuri Battaglia, Giovanni Piva, Giovanni Mosconi, Alessandra Spazzoli, Paolo Ferdinando Bruno, Katia Ambri, Barbara Veterani, Sara Signorotti, Marcora Mandreoli, Renato Mario Rapanà, Maria Teresa Benedetto, Laura Patregnani, Maddalena Zambelli, Gaetano Alfano, Mariacristina Gregorini, Silvia Mattei, Francesca Iannuzzella, Elena Pelizzaro, Luca Camparini, Giuseppe Battaglino, Dino Romanini, Antonio Irlando, Lojze Celik, Giuseppa Natale, Maria Cristina Torre, Ilaria Umbro, Nicola Pirozzi, Loredana Fazzari, Lucia Pantano, Antonio Paone, Marco Galliani, Veronica Baglio, Eleonora Moscaritolo, Sabrina Fierimonte, Maria De Cristofaro, Elena Nebuloso, Paolo Menè, Francesca Romana Festuccia, Giulia Talarico, Claudia Fofi, Maria Elena Bracaccia, Ernesto Anselmo Cioffi, Fabio Mazza, Pasquale Polito, David Micarelli, Roberto Addesse, Lida Tartaglione, Sandro Feriozzi, Francesca Romana Della Rovere, Natalia Chipilova, Micol Manzuoli, Paolo Sacco, Francesca Ansaldo, Chiara Bottaro, Sonia Marre, Francesc Viazzi, Valeria Falqui, Novella Conti, Angelica Parodi, Valentina Zanetti, Francesca Cappadona, Andrea Speciale, Giancarlo Mancuso, Monica Repetto, Emanuela Chiara Vigo, Cairo Montenotte, Anna Maria Murgia, Piero Ruggenenti, Patrizia Ondei, Carmela Giuseppina Condemi, Sivia Bernardi, Francesco Scolari, Paola Gaggia, Federico Alberico, Chiara Manenti, Brunella Valzorio, Corrado Camerini, Agnese Gallico, Michela Tonoli, Federico Daffara, Roberto Zubbani, Simona Guerini, Mattia Zappa, Nicole Zambetti, Alessandra Dalla Gassa, Paola Baldan, Luca Fraizzoli, Sergio Bisegna, Nicola Palmieri, Marco Petrilli, Giusy Mandanici, Francesca Serena Stefani, Valeria Ogliari, Cristina Tantardini, Fabio Malberti, Paola Pecchini, Vincenzo La Milia, Carlo Maria Guastoni, Annalisa Neri, Marina Cornacchiari, Marco Farina, Francesco Barbisoni, Milena Maggio, Mario Cozzolino, Matthias Cassia, Michela Frittoli, Lorenza Magagnoli, Rossella De Leonardis, Roberta Casazza, Simone Vettoretti, Emanuele Grimaldi, Matteo Abinti, Francesca Maria Ida Carminati, Silvia Giuliani, Matteo Benedetti, Nicholas Delfrate, Elisa Colombo, Angela Cervesato, Enrico Eugenio Minetti, Alberto Montoli, Chiara Brunati, Valerae Li Bergolis, Maurizio Galllieni, Cristina Airaghi, Monique Buskermolen, Laura Cosmai, Maria Antonietta Orani, Cristina De Salvo, Giuseppe Vezzoli, Giorgio Slaviero, Chiara Lanzani, Federico Pieruzzi, Gina Contaldo, Barbara Trezzi, Teresa Rampino, Fabrizio Grosjean, Paola Borille, Ciro Esposito, Giuseppe Sileno, Marta Arazzi, Gianvincenzo Melfa, Mariagiulia Tettamanti, Anna Tosetti, Marco D’Amico, Maria Giulia Magatti, Silvia Peiti, Luciano Pedrini, Annalisa Feliciani, Elena Pezzini, Silvio Volmer Bertoli, Daniele Ciurlino, Silvia Tedoldi, Vania Prettico, Giulia Maria Magni, Antonietta Gazo, Maurizio Nai, Silvia Muciaccia, Graziana Battini, Lino Merlino, Paola Casanova, Federica Lencia, Maddalena Ricci, Andrea Ranghino, Mauro Valente, Carolina Finale, Eleonora Guerrini, Marta Canonici, Stefano Santarelli, Rosa Maria Agostinelli, Marina Di Luca, Mauro Martello, Cristina Silvestri, Veronica Bertuzzi, Assunta Cardillo, Valentina Nastasi, Sara Belcastro, Marco Manganaro, Emanuele Luigi Parodi, Valentina Vaccaro, Antonella Giolito, Stefano Cusinato, Michele Battista, Elena Ragazzoni, Paola Marcella Carpani, Emanuele Stramignoni, Silvana Savoldi, Guido Martina, Chiara Deagostini, Marica Magnetti, Stefania Bussolino, Federica Ventrella, Sonia Santi, Marita Marengo, Daniela Falconi, Ilaria Serra, Luca Besso, Davide Diena, Doriana Chiari Notti, Paola David, Luciana Gravellone, Corrado Vitale, Silvia Berutti, Silvia Ganci, Federica Neve Vigotti, Giulio Cesano, Maurizio Borzumati, Patrizia Bio, Stefania Gioira, Oliviero Filiberti, fabiola Pagani, Simonetta Ottone, Carlo Lomonte, Vincenzo Montinaro, Vito Pepe, Elisabetta Manno, Salvatore Di Paolo, Luigi Natalicchio, Carmela Gallo, Francesca pansini, Rossella Varvara, Filomena D’Elia, NIcola Coviello, Concetta Prisciandaro, Lucia Vernò, Fernanda Misceo, Pierfelice Zazzera, Antonella Matrella, Loreto Gesualdo, Anna Maria Di Palma, Virginia Pronzo, Federica Cassone, Salvatore Di Paolo, Tiziana Piccolo, Dora Porcelluzzi, Antonella Di Franco, Mario Giannetto, Chiara Villani, Luigi Vernaglione, Angelo Specchio, Laura Stoico, Giovanni Stallone, Vincenzo Losappio, Barbara Infante, Luigi Morrone, Anna Lisa Marangi, Angela Rodio, Christian D’Altri, Paolo Venerito, Francesca Cianciotta, Filippo Aucella, Gaetano Ferrara, Anna Rachele Nardella, Luigi Francesco Pio Morrone, Maria Grazia Corallo, Giuseppina D’Ettorre, Francesco Caccetta, Davide Gianfreda, Antonello Pani, Gianfranca Cabiddu, Stefania Caria, Maria Maddalena Romano, Ciro Adamo, Giovanni Giorgio Battaglia, Barbara Pocorobba, Emanuela Prencipe, Margherita Saraceno, Luana Scuderi, Salvatore Randone, Francesca Bruno, Rossella Rita Marchese, Antonio Granata, Daniela Puliatti, Carmelita Marcantoni, Luca Zanoli, Ambra D’anca, Domenico Santoro, Guido Gembillo, Alfio Eduardo Giuffrida, Vincenzo Labbozzetta, Angelo Tralongo, Gioacchino Li Cavoli, Carmela Zaggarigo, Paolo Conti, Sofia Giovannini, Silvia Farsetti, Pietro Claudio Giovanni Dattolo, Aris Tsalouchos, Stefano Michelassi, Marco Gallo, Irene Bandor, Nadia Sami, Daniela Riccomi, Lorena Traversari, Maria Grazia Tabbi, Maria Luisa Bonincontro, Vera Bonell, Giuliano Brunori, Diana Zarantonello, Alessandro Laudon, Antonio Selvi, Stefania Santoni, Davide Massimiani, Davide Rossi, Lorenzo Calò, Barbara Rossi, Giuseppe Scaparrotta, Fulvio Fiorini, Valentina Iacono, Carlo Rugiu, Monica Slaviero, Maurizio Nordio, Riccarda Maria Puggia, Adriana Caberlotto, Flavio Scanferla, Vincenzo Casoria, Federica Gramegna, Gina Meneghel, Ilenia Filippi, Anna Giacomini, Claudio Ronco, Anna Giuliani, Sabrina Milan Manani, Monica Zanella
Additional file 2: of Differential synovial tissue biomarkers among psoriatic arthritis and rheumatoid factor/anti-citrulline antibody-negative rheumatoid arthritis
No full textTable S1. Inter-rater agreement coefficients for CD68, CD21, CD20, CD3, CD117, CD138, and CD31 IHC scores. (DOCX 12 kb
Imaging features of adrenal masses
No full textThe widespread use of imaging examinations has increased the detection of incidental adrenal lesions, which are mostly benign and non-functioning adenomas. The differentiation of a benign from a malignant adrenal mass can be crucial especially in oncology patients since it would greatly affect treatment and prognosis. In this setting, imaging plays a key role in the detection and characterization of adrenal lesions, with several imaging tools which can be employed by radiologists. A thorough knowledge of the imaging features of adrenal masses is essential to better characterize these lesions, avoiding a misinterpretation of imaging findings, which frequently overlap between benign and malignant conditions, thus helping clinicians and surgeons in the management of patients. The purpose of this paper is to provide an overview of the main imaging features of adrenal masses and tumor-like conditions recalling the strengths and weaknesses of imaging modalities commonly used in adrenal imaging
The potential role of advanced glycation end products in food allergy pathogenesis
No full textprevalence has dramatically increased in the last two
decades. Among dietary factors, it has been
hypothesized that advanced glycation
endproducts(AGEs), present at high level in junk food, could be
involved in FA pathogenesis. AGEs are a heterogeneous group of
compounds deriving from sugars(sweets and beverages),
autoclaved/processed foods, microwaved foods, more
roasted/barbecued meat. To evaluate the AGEs levels in FA children
compared with healthy controls and subjects with respiratory allergy.
Methods: We evaluated paediatric patients with challenge-proven FA,
children with respiratory allergy(RA) and age and sex-matched healthy
controls. Subcutaneous AGEs levels were evaluated through the AGE
reader. Food-frequency questionnaires were evaluated in all study
subjects.
In vitro studies were performed on human enterocytes(Caco-2 cells)
stimulated with 200 mg/ml of BSA-AGE for 24and48 hours to evaluate
effects on gut barrier function: mucin2(mucus production),
transpithelial electrical resistance(TEER), ZO-1, occludin
expression(intestinal permeability). The direct effects elicited on
peripheral blood mononuclear cells (PBMCs) after the treatment with
200 mg/ml of BSA-AGE for 48hours, 4and 7days of treatment were
also evaluated.
RESULTS: 115 subjects were evaluated and subdivided into 3 groups:
group 1 patients with FA (n=31); group 2 patients with RA (n=18),
group 3 healthy controls (n=66). The consumption of food containing
AGEs was higher in subjects with FA compared to RA children and
healthy controls (p<0.05). FA and RA children presented significant
higher subcutaneous AGEs levels compared to healthy controls
(p<0.05). Linear regression analysis confirmed a significant positive
correlation between subcutaneous levels of AGEs and consumption of
food containing AGEs. Human enterocytes exposed to BSA-AGE
treatment showed a reduction of TEER, of Muc2 and tight junction
proteins (Occludin and ZO-1). Moreover, the treatment with BSA-AGE
on human PBMCs stimulates pro-inflammatory cytokines TNF-α and
Th2 cytokines(IL-5 and IL-13)production , but it was unable to
modulate IL-10 production. Finally, after7days of treatment with BSAAGE, we found a low percentage of proliferating CD4+T.
CONCLUSIONS: Current hypotheses and models of FA do not
adequately explain the dramatic increase observed in the last years
Neuropsychiatric or Behavioral and Psychological Symptoms of Dementia (BPSD): Focus on Prevalence and Natural History in Alzheimer's Disease and Frontotemporal Dementia
No full textNeuropsychiatric or behavioral and psychological symptoms of dementia (BPSD) represent a heterogeneous group of non-cognitive symptoms that are virtually present in all patients during the course of their disease. The aim of this study is to examine the prevalence and natural history of BPSD in a large cohort of patients with behavioral variant of frontotemporal dementia (bvFTD) and Alzheimer's disease (AD) in three stages: (i) pre-T0 (before the onset of the disease); (ii) T0 or manifested disease (from the onset to 5 years); (iii) T1 or advanced (from 5 years onwards). Six hundred seventy-four clinical records of patients with bvFTD and 1925 with AD, from 2006 to 2018, were studied. Symptoms have been extracted from Neuropsychiatric Inventory (NPI) and from a checklist of BPSD for all periods observed. In our population, BPSD affect up to 90% of all dementia subjects over the course of their illness. BPSD profiles of the two dementia groups were similar but not identical. The most represented symptoms were apathy, irritability/affective lability, and agitation/aggression. Considering the order of appearance of neuropsychiatric symptoms in AD and bvFTD, mood disorders (depression, anxiety) come first than the other BPSD, with the same prevalence. This means that they could be an important “red flag” in detection of dementia. With the increase of disease severity, aberrant motor behavior and wandering were significantly more present in both groups. Differences between BPSD in AD and bvFTD resulted only in prevalence: Systematically, in bvFTD, all the symptoms were more represented than in AD, except for hallucinations, depression, anxiety, and irritability. Given their high frequency and impact on management and overall health care resources, BPSD should not be underestimated and considered as an additional important diagnostic and therapeutic target both in patients with AD and bvFTD
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