121 research outputs found
Expression, role and regulation of NK cell immune checkpoints (PD-1, KIRs, NKG2A) in Ovarian Cancer
No full textOutstanding results can be obtained with immune-checkpoints (ICs)/ligands blockade strategies (mainly PD-1/PD-L axis) in immunotherapeutic protocols for aggressive tumors. We previously showed that higher proportions of PD-1+NK cells can be detected in ovarian cancer (OC) patients. Here, we deeply characterized the features of the PD-1+NK cell subset in a large cohort of patients with high-grade serous OC subtype with the aim to dissect the NK/tumor interconnections existing in this TME. We identified two new PD-1+NK subsets occurring only in OC patients, primarily in the peritoneal fluid (PF) compartment, while absent in healthy donors. These subsets show an immature phenotype, characterized by high expression of NKG2A and NKp46, variable expression of KIRs, and low/lack expression of CD57.
Functional analyses revealed that PF-PD-1+NK cells are not anergic, but able to degranulate in response to appropriate stimuli. In addition, we found PD-1+NK cells in different tumor tissue compartments, mainly in metastatic niche, suggesting a role for PD-1 in tumor metastatic progression/promotion. However, even if PD-1+NK cells are functional and infiltrate the tumor, they are unable to produce an efficient anti-tumor response towards OC cells, due to NK cells expressing multiple ICs including PD-1,NKG2A,KIRs, and OC cells expressing both PD-Ls and HLA-I, completely impairing the NK activity. Importantly, our data show that by blocking all the ICs pathways, NK cell activity can be restored. This indicates that a proper use of multiple targeted therapies, including a combination of ICs-inhibitors, represents a possible solution to restore NK cell anti-tumor activity in seropapillary OC subtype
Le tecniche murarie
No full textAnalisi archeologica della muratura dell'abside di S. Eustorgio (Milano
Optimization with genetic algorithms of PVT system global efficiency
No full textPhotovoltaic (PV) solar panels generally produce electricity in
the 6% to 12% efficiency range, the rest being dissipated in
thermal losses. To recover this amount, hybrid photovoltaic
thermal systems (PV/T) have been devised. These are devices
that simultaneously convert solar energy into electricity and
heat. A significant amount of research on PV/T collectors has
been carried out over the last decade and water PV/T glazed flat
plate collector systems turned out to be the most promising to
develop (Zondag[1]). It is thus interesting to study the PV/T
system as part of a closed loop single phase water CDU
(coolant distribution unit) in laminar forced convection. In
particular, the analysis was conducted on the optimal cooling
performance of the thermal part, testing polynomial channel
profiles of varying order (from zero to fourth) for channels of a
real industrial module heat sink, under the following conditions:
ideal flux of 1000 W/m2 on one side, insulation on the opposite
side, periodic conditions on the remaining sides, fully
developed thermal and velocity profile in laminar flow of water.
Through the use of a genetic algorithm, we have optimized the
shape of the channel’s sidewalls in terms of heat transfer
maximization. In terms of Nusselt number, results show that
fourth order profiles are the most efficient. When limits to
allowable pressure loss and module weight are introduced, these
bring generally to a lower efficiency of the system than the
unconstrained case
Performance produttive di cinghiali allevati con mangimi contenenti acidi grassi polinsaturi.
No full textAccumulare e difendere nelle Alpi Carniche dell'IX secolo: gli scavi nel sito fortificato del Broili di Illegio
No full textIl contributo riporta gli esiti delle indagini archeologiche e dell'architettura condotte nel sito fortificato del Broili ad Illegio (Tolmezzo- Udine)
La vivienda: un derecho inaccesible en Córdoba capital
No full textPonencia presentada en el Seminario "Hábitat, Acceso al Suelo y Financiamiento. Córdoba, 2014Fil: Gargantini, Daniela. Universidad Nacional de Córdoba. Facultad de Arquitectura, Urbanismo y Diseño; ArgentinaFil: Martiarena, Miguel. Universidad Nacional de Córdoba. Facultad de Arquitectura, Urbanismo y Diseño; ArgentinaFil: Greppi, Verónica. Universidad Nacional de Córdoba. Facultad de Arquitectura, Urbanismo y Diseño; ArgentinaFil: Peralta, Joaquín. Universidad Nacional de Córdoba. Facultad de Arquitectura, Urbanismo y Diseño; ArgentinaEl creciente proceso de inaccesibilidad a un hábitat digno en nuestras ciudades específicamente a la vivienda- constituye una de las principales evidencias de un modelo de desarrollo urbano que lejos de ser inclusivo, reproduce situaciones de inequidad y exclusión. Ante a este marco contextual, la ponencia que aquí se presenta pretende evidenciar, a partir del análisis crítico y georeferenciado de la interrelación de datos socio-habitacionales, el grado de inaccesibilidad a la vivienda que la ciudad de Córdoba manifiesta. Para ello se analizan pormenorizamente variables consideradas claves en materia de accesibilidad al hábitat, como manera de evidenciar las situaciones de violación al derecho a la vivienda que se manifiestan en la ciudad y hacia las cuales deberían dirigirse las políticas públicas locales a fin de revertirlas.http://ceve.org.ar/documentos/Habitat_acceso_%20al_suelo_financiamiento.pdfFil: Gargantini, Daniela. Universidad Nacional de Córdoba. Facultad de Arquitectura, Urbanismo y Diseño; ArgentinaFil: Martiarena, Miguel. Universidad Nacional de Córdoba. Facultad de Arquitectura, Urbanismo y Diseño; ArgentinaFil: Greppi, Verónica. Universidad Nacional de Córdoba. Facultad de Arquitectura, Urbanismo y Diseño; ArgentinaFil: Peralta, Joaquín. Universidad Nacional de Córdoba. Facultad de Arquitectura, Urbanismo y Diseño; ArgentinaCiencias Sociales Interdisciplinaria
PVT WATER BASED PROTOTYPE CONSTRUCTION AND EXPERIMENTAL EFFICIENCY ANALYSIS FOR A PARTICULAR ITALIAN NORTH-EST LOCATION
No full textPhotovoltaic (PV) solar panels generally produce electricity in the 6% to 15 % efficiency range, the rest of the solar energy
being dissipated in thermal losses. To recover this amount, hybrid photovoltaic thermal systems (PVT) have been devised.
These are devices that simultaneously convert solar energy into electricity and heat. It is thus interesting to study the PVT
system experimentally for a particular climatic condition as part of a closed loop single phase water CDU ( coolant distribution
unit ) in laminar forced convection. Results show that clear advantages regarding increasing open circuit voltage till +30%
with a little decrease of thermal efficiency(±10/15%) are obtained in particular conditions by a single hybrid PVT water panel
compared to separated productions
A new method for oral cancer biomarkers detection with a non-invasive cyto-salivary sampling and rapid-highly sensitive ELISA immunoassay: a pilot study in humans
No full textIntroduction: Oral squamous cell carcinoma (OSCC) accounts for approximately 90% of oral malignancies and has a 5-year mortality rate close to 50%. A consistent part (70%) of all oral cancers is diagnosed at an advanced stage since available screening techniques are ineffective. Therefore, it would be urgent to improve them. The diagnostic gold standard is tissue biopsy with histological and immunohistochemical assessment. This method presents some limitations. Biopsy is invasive and the histopathological evaluation is semi-quantitative, and the absolute abundance of the target cannot be reliably determined. In addition, tissue is highly processed and may lead to loss of information of the natural state. The search for classical and new clinical biomarkers on fragments of tissue/cells collected with a cytobrush is a highly hopeful technique for early detection and diagnosis of OSCC, because of its non-invasive sampling and easy collection method.
Methods: Here we analyzed cytobrush biopsies samples collected from the oral cavity of 15 patients with already diagnosed OSCC by applying an innovative high-sensitivity ELISA technique, in order to verify if this approach may provide useful information for detection, diagnosis, and prognosis of OSCC. To this end, we selected six biomarkers, already used in clinical practice for the diagnosis of OSCC (EGFR, Ki67, p53) or selected based on recent scientific and clinical data which indicate their presence or over-expression in cells undergoing transformation and their role as possible molecular targets in immunecheckpoints blockade therapies (PD-L1, HLA-E, B7-H6).
Results: The selected tumor biomarkers were highly expressed in the tumor core, while were virtually negative in healthy tissue collected from the same patients. These differences were highly statistically significant and consistent with those obtained using the gold standard test clearly indicating that the proposed approach, i.e. analysis of biomarkers by a custom ELISA technique, is strongly reliable.
Discussion: These preliminary data suggest that this non-invasive rapid phenotyping technique could be useful as a screening tool for phenotyping oral lesions and support clinical practice by precise indications on the characteristics of the lesion, also with a view to the application of new anti-tumor treatments, such as immunotherapy, aimed at OSCC patients
Uso de recursos genéticos argentinos bajo el protocolo de Nagoya. Experiencia del INTA con la provincia de Misiones
No full textDesde su creación en 2004, el Instituto de Floricultura del INTA (IF) se encuentra trabajando en convenios de vinculación tecnológica con empresas multinacionales y con los estados provinciales argentinos con el objetivo de desarrollar nuevos cultivares ornamentales utilizando para ello recursos genéticos nativos de Argentina, labor que es llevada a cabo dentro del marco establecido por el Convenio de Diversidad Biológica (CBD) de la Organización de Naciones Unidas (ONU). En este marco se destacan los resultados obtenidos por Instituto de Floricultura de INTA con respecto a la obtención de variedades ornamentales en los géneros Mecardonia,
Calibrachoa y Begonia provenientes de recursos genéticos nativos de Misiones, comercializadas a nivel nacional e internacional, generando regalías que son compartidas entre las partes, en concordancia con el Protocolo de Nagoya. En este trabajo se destaca un vínculo virtuoso entre el INTA y la provincia de Misiones que permitió la obtención en el año 2023 del certificado de cumplimiento del Protocolo de Nagoya, demostrando así el compromiso con la protección de la biodiversidad y el uso sostenible de los recursos naturales. Este hito no sólo resalta la responsabilidad asumida con la ética ambiental, sino también la capacidad para innovar y
posicionar a Argentina como un socio estratégico de empresas internacionales en el uso comercial de recursos genéticos.Instituto de FloriculturaFil: Soto, Maria Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; ArgentinaFil: Greppi, Julian Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentin
Depicting the Urban Landscape. Enhancing the Cultural Heritage of Fragile Areas with Participatory Mapping Processes
No full textThe contribution suggests reflecting upon participatory mapping of cultural values processes contained in everyday landscapes, particularly fragile environments such as peripheral urban areas. The theme is addressed starting from the reexamination of Politecnico di Milano research experience which focused on the involvement of younger generations in landscape education and enhancement of the widespread cultural heritage processes. From this experimental project, we developed tools and methodologies to support institutions in standardising local heritage education. Within this framework, the participatory mapping tradition becomes an important reference for its subjective and interdisciplinary aspect and ability to represent the community’s spatial knowledge, because it offers cues for technological integration in processes for inexperienced users. Mapping used here is a reading, interpreting, communicating and sharing process which involves multiple players and educational frameworks. Drafting such maps combines traditional methodologies and innovative technologies and allows the development of different activities and products including learning tours, visual storytelling, sketches, mind maps, interactive digital maps, Geographic Information Systems, virtual reality environments, interactive digital tourist tours and gamification apps. The availability of these tools allows young citizens to become active players in the knowledge and communication processes, to strengthen their sense of belonging to places, to develop active citizenship, to foster intergenerational and intercultural dialogues, and to protect the cultural landscape as a common good
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