1,721,174 research outputs found
Development of a Peptide Nucleic Acid/HPLC method for the detection of traces of potentially allergenic hazelnut in foodstuff
No full textChiral Peptide Nucleic Acids: Design, Synthesis, Optical Purity and Hybridization Properties
No full textChiral Separation of Unmodified alfa-Hydroxy Acids by Ligand Exchange HPLC Using Chiral Copper(II) Complexes of (S)-Phenylalaninamide as Additives to the Eluent
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Enhanced recognition of cystic fibrosis W1282X DNA point mutation by chiral peptide nucleic acid probes by a surface plasmon resonance biosensor
No full textPeptide nucleic acids (PNAs) containing an insert of three chiral monomers based on D-lysine ('chiral box') were synthesized and used as probes in Biospecific Interaction Analysis (BIA) for the recognition of DNA containing the W1282X point mutation of the cystic fibrosis gene. Hybridization experiments carried out in solution showed enhanced mismatch recognition when compared with the analogous achiral PNAs and oligonucleotides. The signal intensity was lower, but the selectivity of the Biacore response was found to be much higher than that observed with achiral PNAs. The newly designed chiral PNA probes were also found to hybridize with a 1:1 mixture of normal (N-W1282X) and mutated (M-W1282X) DNA oligomers immobilized on the biosensor, thus allowing discrimination not only between a normal and a mutated sequence (healthy/homozygous), but also between homo- and heterozygous individuals. These results suggest that 'chiral box' PNAs are potential powerful tools for the analysis of single point mutations of biological/biomedical relevance. Copyright 2003 John Wiley & Sons, Ltd
Detection of DNA amplicons and kinetics of PNA/DNA hybridization in buffer of different ionic strength by using surface plasmon optical techniques. (2005), COLL-249. CODEN: 69GQMP AN 2005:188952 CAPLUS
No full textTwo-Dimensional High-Performance Liquid Chromatographic System for the Determination of Enantiomeric Excess in Complex Amino Acid Mixtures. Single Amino Acid Analysis.
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Simultaneous HPLC-FLD Detection of Type A and Type B Trichothecenes as 3-cumaroyl-derivatives.
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