1,720,961 research outputs found
Involvement of a novel Pseudomonas protegens strain associated with entomopathogenic nematode infective juveniles in insect pathogenesis
Full text linkBACKGROUND: The bioinsecticidal action of entomopathogenic nematodes (EPNs) typically relies on their symbiosis with core bacteria. However, recent studies highlighted the possible involvement of other noncore species. We have recently isolated a novel Pseudomonas protegens strain as a major agent of septicaemia in larvae of the wax moth, Galleria mellonella, infected with a soil-dwelling Steinernema feltiae strain. The actual role of this bacterium in entomopathogenesis was investigated. RESULTS: The association of P. protegens with nematodes appeared to be robust, as supported by its direct and repeated isolation from both nematodes and insect larvae infected for several consecutive generations. The bacterium appeared to be well-adapted to the insect haemocoel, being able to proliferate rapidly after the injection of even a small amount of living cells [100 colony forming units (CFU)] to a larva, causing its fast death. The bacterium also was able to act by ingestion against G. mellonella larvae [median lethal concentration (LC50) = 4.0 × 107 CFU mL–1], albeit with a slower action, which supports the involvement of specific virulence factors (e.g. chitinases, Fit toxin) to overcome the intestinal barrier to the haemocoel. Varying levels of bacterial virulence were observed on diverse target Diptera and Lepidoptera. CONCLUSION: The soil-dwelling bacterium P. protegens appears to have evolved its own potential as a stand-alone entomopathogen, yet the establishment of an opportunistic association with entomoparasitic nematodes would represent a special competitive advantage. This finding contributes to a deeper understanding of the nematode–bacteria biocontrol agent complex and the deriving paradigm of their use as biological control agents. © 2022 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry
Implicazioni molecolari dell’interferenza causata dai conidi aeriferi di Beauveria bassiana sull’ovideposizione di Ceratitis capitata
No full textProteomics of Brevibacillus laterosporus and its insecticidal action against noxious Diptera
No full textStudies on the regulatory regions of a DHFR/TS gene of Arabidopsis thaliana controlling its expression in root meristems.
No full textExploring the bioactivity of a novel pine wood distillate (PWD) for plant growth and protection
Full text linkExploiting plants by-product properties for combined crop growth regulation and pest management could represent a strategy for a more sustainable agriculture. The present study investigated the potential activity (at different product concentrations) of a novel pine wood distillate (PWD) obtained as an industrial by-product, on multiple targets in the agroecosystem. In the weed species, Sylibum marianum, PWD stimulated a more than twofold increase of seed germination and seedling development, while it was able to inhibit by up to 70% the growth of the soil-borne plant pathogen Fusarium culmorum on durum wheat seedlings. PWD was also able to induce behavioural changes in mature females of the fruit fly Ceratitis capitata, with a significant reduction in the visit and oviposition rate on treated orange fruits (53% and 62% less, respectively). Analysis of PWD chemical composition suggested a role of phenolic compounds in the observed species-specific effects. Taken together, these results support a multivalent exploitation of wood distillates in the management of important crops of the Mediterranean area, aligning with both circular economy and environmental protection principles.Please confirm if the author names are presented accurately and in the correct sequence (given name, middle name/initial, family name). Author 3 Given name: [Maria Giovanna] Last name [Marche]. Also, kindly confirm the details in the metadata are correct.Yes, it is correc
Pseudomonas protegens as a stand-alone bacterial biocontrol agent or in association with EPNs
No full textPseudomonas protegens as a stand-alone bacterial biocontrol agent or in association with EPN
Attività insetticida e caratterizzazione molecolare di un isolato di Bacillus thuringiensis efficace su larve di Ceratitis capitata (Wied.)
No full textInhibition of Paenibacillus larvae by an extracellular protein fraction from a honeybee-borne Brevibacillus laterosporus strain
No full textThe inhibitory action that a Brevibacillus laterosporus strain isolated from the honeybee body causes against the American Foulbrood (AFB) etiological agent Paenibacillus larvae was studied by in-vitro experiments. A protein fraction isolated from B. laterosporus culture supernatant was involved in the observed inhibition of P. larvae vegetative growth and spore germination. As a result of LC-MS/MS proteomic analyses, the bacteriocin laterosporulin was found to be the major component of this fraction, followed by other antimicrobial proteins and substances including lectins, chaperonins, various enzymes and a number of putative uncharacterized proteins. The results obtained in this study highlight the potential of B. laterosporus as a biological control agent for preserving and improving honeybee health
Genome sequence of beauveria bassiana strain ATCC 74040, a widely employed insect pathogen
No full textThe broad-spectrum insecticidal activity of Beauveria bassiana strain ATCC 74040 is well documented. The whole-genome sequence of this strain is reported here, revealing a plethora of genes related to its insecticidal potential and providing new insights on the mechanism of action
- …
