16 research outputs found
Albis Torres, una poeta con alas
No full textThe work analyzes In Albis, a collection of works by Cuban poet Albis Torres, a writer barely published in her country and largely overlooked by literary critics. The contribution of the author to the 1980’s poet generation is shown; the members of this generation started to write from their own subjectivities, thus far ignored by the revolution’s literary panorama. Within the alluded spaces and subjects, and which legitimated in this decade, the female discourse is stressed, visibly present in the writing of Albis Torres, who by means of different images referring to flying expresses her refection on those locations that try to contain her in a space limited and conditioned by social rules and where she cannot fully develop as a woman or writer.Se analiza la obra In Albis, que reúne la producción de la poeta cubana Albis Torres, escritora apenas publicada en su país y mayormente omitida por la crítica literaria. Se muestra el aporte de la autora a la generación poética de los años ochenta, cuyos integrantes comienzan a escribir desde sus propias subjetividades, hasta el momento omitidas del panorama literario de la revolución. Dentro de los espacios y sujetos aludidos y que se legitiman en esta década resalta el discurso femenino, visiblemente presente en la escritura de Albis Torres, quien mediante diferentes imágenes referentes al acto de volar expresa su rechazo a aquellos emplazamientos que tratan de contenerla en un espacio condicionado y limitado por las reglas sociales, y en donde no puede desarrollarse plenamente como mujer o escritora
Un ultimo comentario «En el dudoso mar de las obras de don Luis de Góngora». Salcedo Coronel ante el Panegírico al duque de Lerma
No full textAlmost twenty years after editing the glosses on the Fábula de Polifemo y Galatea, the work that introduced him to the relevant discussion about Góngora’s lyrical poetry, Salcedo Coronel published the second part of the Obras de don Luis de Góngora comentadas, a final volume whose last pages included the Panegírico al duque de Lerma. This annotated edition helps to identify a model based on relevant works ascribed to the genre. Through this model, the Sevillian author improved and added certain determining characteristics to his first printed version.Casi veinte años después de las glosas alrededor de la Fábula de Polifemo y Galatea, aquellas que le incorporarían a la relevante discusión en torno a la lírica gongorina, Salcedo Coronel vería impresa la segunda parte de las Obras de don Luis de Góngora comentadas, donde las explicaciones sobre el Panegírico al duque de Lerma ocuparían las últimas páginas. Este comentario nos sirve para observar un modelo que, si bien asentado en unas bases establecidas en destacadas obras adscritas al género, el sevillano habría integrado a la materia tratada, variado respecto a su primer impreso y añadido ciertas características determinantes
Estudio técnico de un sondeo de captación de agua subterráneas y construcción de una balsa para el abastecimiento de usos agrícolas en el T. M. de Bailén (Jaén)
No full text[es] Este Trabajo de Fin de Grado (TFG) se presenta bajo la modalidad de “estudio técnico”. El proyecto elegido para desarrollar es un un sondeo de captación de aguas subterráneas y construcción de una balsa para el abastecimiento de usos agrícolas en el término municipal (en adelante, T.M.) de Bailén (Jaén). El autor ha establecido íntegramente las características y planteamientos iniciales del proyecto, basándose en la cartografía y antecedentes accesibles, para realizar un estudio técnico sobre la realización del sondeo y balsa de abastecimiento mencionados.[en] This final degree work (TFG) is presented under the modality of "technical study". The project chosen to develop is a groundwater collection well and construction of a reservoir to supply agricultural uses in the municipal area (hereinafter, T.M.) of Bailén (Jaén). The author has fully established the characteristics and initial approaches of the project, based on the cartography and accessible background, to carry out a technical study on the completion of the aforementioned well and supply pond
Estudio técnico de un sondeo de captación de aguas subterráneas y construcción de una balsa para el abastecimiento de usos agrícolas en T.M. de Úbeda
No full text[es] Este Trabajo de Fin de Grado (TFG) se presenta bajo la modalidad de “estudio técnico”. El proyecto elegido para desarrollar es un un sondeo de captación de aguas subterráneas y construcción de una balsa para el abastecimiento de usos agrícolas en el término municipal (en adelante, T.M.) de Úbeda (Jaén). El autor ha establecido íntegramente las características y planteamientos iniciales del proyecto, basándose en la cartografía y antecedentes accesibles, para realizar un estudio técnico sobre la realización del sondeo y balsa de abastecimiento mencionados. Como innovación respecto a otros trabajos desarrollados en la misma temática, se ha empleado software de metodología BIM (REVIT 2023), para diseñar el movimiento de tierras de la balsa sobre una topografía virtual.[en] This final degree work (TFG) is presented under the modality of "technical study". The project chosen to develop is a groundwater collection well and construction of a reservoir to supply agricultural uses in the municipal area (hereinafter, T.M.) of Úbeda (Jaén). The author has fully established the characteristics and initial approaches of the project, based on the cartography and accessible background, to carry out a technical study on the completion of the aforementioned well and supply pond. As an innovation compared to other works carried out on the same subject, BIM methodology software (REVIT 2023) has been used to design the earthworks of the pond on a virtual topography
Transcriptional regulation of the equol biosynthesis gene cluster in Adlercreutzia equolifaciens DSM19450T
No full text© The Author(s).Given the emerging evidence of equol’s benefit to human health, understanding its synthesis and regulation in equol-producing bacteria is of paramount importance. Adlercreutzia equolifaciens DSM19450T is a human intestinal bacterium—for which the whole genome sequence is publicly available—that produces equol from the daidzein isoflavone. In the present work, daidzein (between 50 to 200 μM) was completely metabolized by cultures of A. equolifaciens DSM19450T after 10 h of incubation. However, only about one third of the added isoflavone was transformed into dihydrodaidzein and then into equol. Transcriptional analysis of the ORFs and intergenic regions of the bacterium’s equol gene cluster was therefore undertaken using RT-PCR and RT-qPCR techniques with the aim of identifying the genetic elements of equol biosynthesis and its regulation mechanisms. Compared to controls cultured without daidzein, the expression of all 13 contiguous genes in the equol cluster was enhanced in the presence of the isoflavone. Depending on the gene and the amount of daidzein in the medium, overexpression varied from 0.5- to about 4-log10 units. Four expression patterns of transcription were identified involving genes within the cluster. The genes dzr, ddr and tdr, which code for daidzein reductase, dihydrodaidzein reductase and tetrahydrodaidzein reductase respectively, and which have been shown involved in equol biosynthesis, were among the most strongly expressed genes in the cluster. These expression patterns correlated with the location of four putative ρ-independent terminator sequences in the cluster. All the intergenic regions were amplified by RT-PCR, indicating the operon to be transcribed as a single RNA molecule. These findings provide new knowledge on the metabolic transformation of daidzein into equol by A. equolifaciens DSM19450T, which might help in efforts to increase the endogenous formation of this compound and/or its biotechnological production.This study was partially supported by projects from MINECO (AGL2014-57820-R) and Asturias Principality (IDI/2018/000114). LV was supported by a contract from the FPI Program of MINECO (BES-2015-072285). ABF was supported by a research contract from a CSIC project (201870E003).Peer reviewe
Screening sourdough samples for gliadin-degrading activity revealed Lactobacillus casei strains able to individually metabolize the coeliac disease-related 33-mer peptide
No full textA selective culture medium containing acid-hydrolyzed gliadins as the sole nitrogen source was used in the search for sourdough-indigenous lactic acid bacteria (LAB) with gliadin-metabolizing activity. Twenty gliadin-degrading LAB strains were isolated from 10 sourdoughs made in different ways and from different geographical regions. Fifteen of the 20 isolated strains were identified as Lactobacillus casei, a species usually reported as subdominant in sourdough populations. The other five gliadin-degrading strains belonged to the more commonly encountered sourdough species Leuconostoc mesenteroides and Lactobacillus plantarum. All these strains were shown to be safe in terms of their resistance to antimicrobial agents. When individually incubated with the α2-gliadin-derived immunotoxic 33-mer peptide (97.5 ppm), half of the L. casei strains metabolized at least 50% of it within 24 h. One strain metabolized 82% of the 33-mer peptide within 8 h, and fully made it disappear within 12 h. These results reveal for the first time the presence in sourdough of proteolytic L. casei strains with the capacity to individually metabolize the coeliac disease-related 33-mer peptide.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author
Factores asociados a placa blanda en pacientes con score de calcio igual a cero
No full textBackground: The coronary artery calcium score is used for risk stratification in asymptomatic patients. Although coronary arterydisease can occur in the absence of coronary artery calcifications, no conditions associated with the presence of soft non-calcifiedplaques have been described in this scenario, beyond the presence of symptoms.Objectives: The aim of this study was to determine the associations between non-calcified plaques and independent variables inpatients with coronary artery calcium score of zero.Methods: Consecutive patients with coronary artery score of zero Agatston units who also underwent computed tomography coronaryangiography were included in the study. Univariate logistic regression analysis was used to find associations. (15) Sensitivity,specificity, positive predictive value (PPV), negative predictive value (NPV), positive likelihood ratio (LH+) and negative likelihoodratio (LH-) were calculated.Results: Among a total of 93 patients, 10% (n = 9) presented non-calcified plaque. A positive exercise stress test was associated withplaques of any degree of severity (OR 6.5; 95% CI, 1.3-33, p = 0.02). This association persisted for non-severe plaques when the positiveexercise stress test was combined with a negative myocardial perfusion SPECT or stress echocardiography for ischemia (OR,12.4; 95% CI 1.5-101, p = 0.02). Sensitivity and specificity of ST-segment depression for non-calcified plaque of any degree of severitywas 44.4% and 86%, respectively, with NPV of 94%, PPV of 25%, LR+ of 3.11 and LR– of 0.65.Conclusions: ST-segment depression could be associated with non-calcified plaques in patients without coronary artery calcifications,even with normal exercise stress myocardial perfusion or wall motion (non-obstructive disease).Introducción: El score de calcio (SC) es una prueba utilizada en la estratificación de riesgo de pacientes asintomáticos. Aunque la enfermedad coronaria puede ocurrir en ausencia de calcificaciones, no se han descripto afecciones asociadas a la presencia de placa blanda (PB) en este contexto, más allá de la presencia de síntomas. Objetivos: determinar predictores clínicos de PB y su eventual precisión diagnóstica en pacientes con un SC de cero. Materiales y métodos: Se incluyeron pacientes consecutivos con un SC de 0 Unidades Agatston que se hubieran realizado además una angiotomografía coronaria. Se determinaron predictores a partir de tres modelos de análisis multivariado. Se calculó la sensibilidad, especificidad, VPN, VPP, + LR y - LR. Resultados: Se incluyeron 93 pacientes. El 10% (n = 9) presentaron PB. La ergometría positiva fue predictor independiente de placas de cualquier severidad (OR 6.5; IC del 95%: 1.3-33, p = 0.02). El poder predictivo persistió para placas no severas cuando se combinó la ergometría con perfusión miocárdica SPECT o ecocardiograma estrés negativos para isquemia (OR 12.4 IC 95% 1.5 - 101, p = 0.02). La sensibilidad y la especificidad del infradesnivel del ST para placa blanda de cualquier grado de severidad fue 44.4% y 86%, respectivamente. El VPN fue del 94% y el VPP del 25%, + LR fue de 3.11 y -LR fue de 0.65. Conclusiones: El infradesnivel del ST podría ser predictor de PB en pacientes sin calcificaciones coronarias, incluso en contexto de perfusión miocárdica o ecocardiograma estrés normales (enfermedad no obstructiva)
Absence of Myocardial Dysfunction during Cold Pressor Stress Echocardiography in Patients with Endothelial Dysfunction
No full textMyocardial perfusion cold pressor test 99mTc MIBISPECT detects both microvascular and epicardial abnormal reactivity in patients with endothelial dysfunction and angiographically normal coronary arteries with intracoronary acetylcholine vasoconstriction
No full textTranscriptome profiling of TDC cluster deletion mutant of Enterococcus faecalis V583
No full textAbstractThe species Enterococcus faecalis is able to catabolise the amino acid tyrosine into the biogenic amine tyramine by the tyrosine decarboxilase (TDC) pathway Ladero et al. (2012) [1]. The TDC cluster comprises four genes: tyrS, an aminoacyl-tRNA synthetase-like gene; tdcA, which encodes the tyrosine decarboxylase; tyrP, a tyrosine/tyramine exchanger gene and nhaC-2, which encodes an Na+/H+ antiporter and whose role in the tyramine biosynthesis remains unknown [2]. In E. faecalis V583 the last three genes are co-transcribed as a single polycistronic mRNA forming the catabolic operon, while tyrS is transcribed independently of the catabolic genes as a monocistronic mRNA [2]. The catabolic operon is transcriptionally induced by tyrosine and acidic pH. On the opposite, the tyrS expression is repressed by tyrosine concentrations [2]. In this work we report the transcriptional profiling of the TDC cluster deletion mutant (E. faecalis V583 ΔTDC) [2] compared to the wild-type strain, both grown in M17 medium supplemented with tyrosine. The transcriptional profile data of TDC cluster-regulated genes were deposited in the Gene Expression Omnibus (GEO) database under accession no. GSE77864
