225 research outputs found
La legislazione del 18 ottobre 532. Le constitutiones ad commodum propositi operis pertinentes: gli esempi di C. 3, 10, 3 e C. 8, 37 (38), 15 The Justinian legislation of 18th October 532. The constitutiones ad commodum propositi operis pertinentes: the examples of C. 3, 10, 3 e C. 8, 37 (38), 15
No full textThis research, as part of a broader work devoted to the constitutiones ad commodum propositi operis pertinentes issued on 18th October 532, is directed to the analysis of C. 3, 10, 3 and C. 8, 37 (38), 15. In particular, considering it plausible that both fall precisely within the last 'cluster' of constitutiones ad commodum, the author attempts to identify the contexts in which, as part of the perusal of ancient jurisprudential materials, the compilers and chancellery may have drawn the cue to make the two normative interventions. The hypothesis is that both constitutions derive from the reading of the libri ad Sabinum and may show that by the date of 18th October 532 the probing work done by the compilers on the commentaries devoted to ius civile, as indeed also that of the great commentaries ad edictum, was nearing completion or, perhaps, had already been finished
Mitochondrial DNA as inflammatory DAMP: a warning of an aging immune system?
No full textSenescence of the immune system is characterized by a state of chronic, subclinical, low-grade inflammation termed 'inflammaging', with increased levels of proinflammatory cytokines, both at the tissue and systemic levels. Age-related inflammation can be mainly driven by self-molecules with immunostimulant properties, named Damage/death Associated Molecular Patterns (DAMPs), released by dead, dying, injured cells or aged cells. Mitochondria are an important source of DAMPs, including mitochondrial DNA - the small, circular, double-stranded DNA molecule found in multiple copies in the organelle. mtDNA can be sensed by at least three molecules: the Toll-like receptor 9, the NLRP3 inflammasomes, and the cyclic GMP-AMP synthase (cGAS). All these sensors can lead to the release of proinflammatory cytokines when engaged. The release of mtDNA by damaged or necrotic cells has been observed in several pathological conditions, often aggravating the course of the disease. Several lines of evidence indicate that the impairment of mtDNA quality control and of the organelle homeostasis associated with aging determines an increase in the leakage of mtDNA from the organelle to the cytosol, from the cell to the extracellular space, and into plasma. This phenomenon, mirrored by an increase in mtDNA circulating levels in elderly people, can lead to the activation of different innate immune cell types, sustaining the chronic inflammatory status that is characteristic of aging
Modulation of the expression of galactose-specific receptors on kupffer cells after partial hepatectomy and zymosan stimulation
No full textProteasome inhibition restores accumulation of HSP72 and HuR proteins in human fibroblasts aged in vitro
No full textElevated maternal placental protein 13 serum levels at term of pregnancy in postpartum major hemorrhage (>1000Â mLs). A prospective cohort study
No full textProblem: To compare placental protein 13 (PP13) levels in the serum of women with primary postpartum hemorrhage (PPH) with a control population. Methods: A prospective cohort study was conducted between May 2014 and May 2016 and included 435 pregnant women at term (38 weeks gestation) without any known risk factor and with normal labor. Multiples of median (MoM) were used to evaluate differences of the PP13 values between cases and controls. PP13 concentrations were adjusted for maternal and neonatal weight. Multivariable analysis was used to detect independent contribution of predictors of PPH. Results: Fifteen had a major PPH >1000 mLs and represented the cases of the study. They were matched with 399 controls. Twenty-one patients who had a minor PPH (500-1000 mLs) were excluded. The mean observed rank in the PPH group was higher than that of controls (28.5 vs 13.5, P-value=.01). PP13 MoM values adjusted for maternal weight were higher than expected being 1.44±0.45 in PPH cases and 1.00±0.59 in controls (P-value.008). This difference was still significant even after adjustment for neonatal weight that represented a confounding variable. Conclusion: Higher PP13 levels are independently associated with major PPH >1000 mLs
Differential Expression of Lonp1 Isoforms in Cancer Cells
No full textLonp1 is a mitochondrial protease that degrades oxidized and damaged proteins, assists protein folding, and contributes to the maintenance of mitochondrial DNA. A higher expression of LonP1 has been associated with higher tumour aggressiveness. Besides the full-length isoform (ISO1), we identified two other isoforms of Lonp1 in humans, resulting from alternative splicing: Isoform-2 (ISO2) lacking aa 42-105 and isoform-3 (ISO3) lacking aa 1-196. An inspection of the public database TSVdb showed that ISO1 was upregulated in lung, bladder, prostate, and breast cancer, ISO2 in all the cancers analysed (including rectum, colon, cervical, bladder, prostate, breast, head, and neck), ISO3 did not show significant changes between cancer and normal tissue. We overexpressed ISO1, ISO2, and ISO3 in SW620 cells and found that the ISO1 isoform was exclusively mitochondrial, ISO2 was present in the organelle and in the cytoplasm, and ISO3 was exclusively cytoplasmatic. The overexpression of ISO1 and, at a letter extent, of ISO2 enhanced basal, ATP-linked, and maximal respiration without altering the mitochondria number or network, mtDNA amount. or mitochondrial dynamics. A higher extracellular acidification rate was observed in ISO1 and ISO2, overexpressing cells, suggesting an increase in glycolysis. Cells overexpressing the different isoforms did not show a difference in the proliferation rate but showed a great increase in anchorage-independent growth. ISO1 and ISO2, but not ISO3, determined an upregulation of EMT-related proteins, which appeared unrelated to higher mitochondrial ROS production, nor due to the activation of the MEK ERK pathway, but rather to global metabolic reprogramming of cells
Leukocytoclastic vasculitis associated with multifocal sensory neuropathy responsive to intravenous immunoglobulins: a case report
No full textLeukocytoclastic vasculitis (LCV) is a common form of small-vessel vasculitis, which commonly presents as palpable purpura or petechiae, caused by deposition of circulating immune complexes on vessels walls that attracts granulocytes which damage the vascular endothelium and leading to erythrocytes extravasation. The skin is the most commonly involved organ, but also renal, gastrointestinal, pulmonary, cardiovascular and neurological systems may be affected. Skin lesions may be the initial signs of systemic vasculitis. Systemic symptoms may be present, such us fever, myalgia, abdominal pain and arthralgia. The presence of neuropathy/mononeuritis multiplex is expression of severe vasculitic involvement. Herein, we describe the case of a patient with leucocytoclastic vasculitis associated to sensitive neuropathy, responsive to intravenous immunoglobulins (IVIg) therapy, after the failure of classic systemic treatments
Morphometric and Vascular Modifications of the Clitoris During Pregnancy : a Longitudinal, Pilot Study
No full textDuring pregnancy, women go through a series of physical and emotional changes that may have an impact on their sexuality. The aim of the study was to examine modifications in sexual function during pregnancy by means of translabial ultrasonography and administration of questionnaires on sexual activity. Eighteen healthy and adult (25–35 years) pregnant women without sexual dysfunction and with a stable heterosexual relationship were examined at 10–12, 18–20, and 30–32 weeks of gestation. Patients underwent ultrasonographic translabial clitoral volume and labia minora thickness measurements and color Doppler assessment of the dorsal clitoral and posterior labial arteries. On the same day, each patient completed the two-factor Italian McCoy Female Questionnaire (MFSQ) and the Female Sexual Function Index (FSFI). The mean clitoral body volume progressively increased during pregnancy, and color Doppler analysis of the dorsal clitoral artery showed a significant decrease in the pulsatility index from the first to the third trimester. Similarly, the labia minora thickness increased and the posterior labial artery pulsatility index progressively decreased throughout gestation. The MFSQ score for sexuality and partnership and the number of intercourses/week did not significantly change during the study period. However, the MFSQ score for partnership reached the lowest value in the third trimester of pregnancy. Analysis of the FSFI data showed no significant differences throughout pregnancy for the majority of the domains (sexual desire, arousal, orgasm, lubrication, and pain), with the exception of the satisfaction item, which decreased during the third trimester. A significant proportion of women are concerned that changes in their body during pregnancy may have a detrimental effect on sexuality. Despite significant changes in genital morphology and vascularity throughout gestation, these modifications do not seem to have an impact on sexual function in pregnancy
Matteolo Mattioli (Mattheolus Perusinus) i jego mnemotechniczny traktat „De memoria augenda” w kontekście polskim przełomu XV i XVI w.
No full text"Matteolo Mattioli (Matheolus Perusinus) and His Mnemotechnic Treatise De Memoria
augenda in the Polish Context of the Turn of the Sixteenth Century"
The main objective of the study is to present a fifteenth-century treatise by Matteolo Mattioli
(most often referred to in the literature as Matheolus Perusinus) entitled De memoria augenda in the context of the humanist culture at the turn of the sixteenth century in Poland, and the Kraków circles especially. The study introduces the author and discusses the treatise itself, something the Polish literature devoted to the subject has not done in much detail so far (to this end, we draw mainly on the works Regina Lupi, Sabine Heimann-Seelbach, Helga Hajdu and Mary Carruthers). It then discusses two editions which are strictly related to Polish culture (Leipzig 1495 and 1508 with an epigram and dedication by Andrzej Boner, brother of Jan Boner, as well as Kraków 1531 with a dedication of Jan Wiewiórka). Based on the dedication and a woodcut with the Rola coat of arms present in the book, it was possible to determine the author of the dedication as Stanisław of Łowicz,
humanist, doctor of medicine and professor at Kraków University as well as establish a new date for the Kraków edition made by Florian Ungler publishing house. Mattioli’s treatise is also put in the context of two other mnemotechnical texts from the early sixteenth century – Opusculum de arte memorativa (Kraków, Kasper Hochfeder, 1504) by Jan Szklarek and Artis memorativae naturalis et artificialis, facilis et verax traditio by Laurent Fries (Strassburg, Johann Grüninger, 1523)
The Utility of Intraoperative Contrast-enhanced Ultrasound for Immediate Treatment of Type Ia Endoleak during EVAR: Initial Experience
No full textObjectives Type Ia endoleak (EL) after endovascular abdominal aortic repair (EVAR) may be misdiagnosed at completion angiography. Intraoperative contrast-enhanced ultrasound (CEUS) may play a role in early detection and immediate treatment of type Ia EL. Methods From January 2017 to April 2018, patients treated with EVAR underwent intraoperative CEUS. After endograft deployment and ballooning, digital subtraction angiography (DSA) and intraoperative CEUS were performed in a blinded fashion. All cases of type Ia EL at DSA or CEUS were considered. Results Type Ia EL detected at intraoperative CEUS and undetected at DSA was defined in 2 patients. The former was solved with intraoperative re-ballooning; in the latter case, a Palmaz stent deployment was required. The resolution of type Ia EL was detected at intraoperative CEUS control and post-operative computed tomography angiography (CTA). In another patient, the DSA detected a type Ia EL, but intraoperative CEUS reveal a type II EL from lumbar arteries. Post-operative CTA confirm the type II EL. Conclusions The reported cases prove the clinical utility of the intraoperative CEUS, permitting the early identification of 2 type Ia EL. In addition, the intraoperative CEUS is useful in case of dubious type Ia EL at DSA, avoiding unnecessary intraoperative adjunctive procedure or post-operative CTA
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