33 research outputs found
Everolimus is a potent inhibitor of activated hepatic stellate cell functions in vitro and in vivo , while demonstrating anti-angiogenic activities
No full textProgression of liver fibrosis to HCC (hepatocellular carcinoma) is a very complex process which involves several
pathological phenomena, including hepatic stellate cell activation, inflammation, fibrosis and angiogenesis.
Therefore inhibiting multiple pathological processes using a single drug can be an effective choice to curb the
progression of HCC. In the present study, we used the mTOR inhibitor everolimus to observe its effect on the in vitro
activation of hepatic stellate cells and angiogenesis. The results of the present study demonstrated that everolimus
treatment blocked the functions of the immortalized human activated hepatic stellate cell line LX-2 without affecting
the viability and migration of primary human stellate cells. We also observed that treatment with everolimus
(20 nM) inhibited collagen production by activated stellate cells, as well as cell contraction. Everolimus treatment
was also able to attenuate the activation of primary stellate cells to their activated form. Angiogenesis studies
showed that everolimus blocked angiogenesis in a rat aortic ring assay and inhibited the tube formation and
migration of liver sinusoidal endothelial cells. Finally, everolimus treatment reduced the load of tumoral
myofibroblasts in a rat model of HCC. These data suggest that everolimus targets multiple mechanisms, making it a
potent blocker of the progression of HCC from liver fibrosis
Chicken chorioallantoic membrane as a reliable model to evaluate osteosarcoma—an experimental approach using SaOS2 cell line
No full textIn ovo administration of human recombinant leptin shows dose dependent angiogenic effect on chicken chorioallantoic membrane
No full textBACKGROUND: Leptin, the cytokine produced by white adipose tissue is known to regulate food energy homeostasis through its hypothalamic receptor. In vitro studies have demonstrated that leptin plays a major role in angiogenesis through binding to the receptor Ob-R present on ECs by stimulating and initiating new capillary like structures from ECs. Various in vivo studies indicate that leptin has diverse effect on angiogenesis. A few reports have showed that leptin exerts pro angiogenic effects while some suggested that it has antiangiogenic potential. It is theoretically highly important to understand the effect of leptin on angiogenesis to use as a therapeutic molecule in various angiogenesis related pathological conditions. Chicken chorio allantoic membrane (CAM) on 9th day of incubation was incubated with 1, 3 and 5 μg concentration of HRL for 72 h using gelatin sponge. Images where taken after every 24 h of incubation and analysed with Angioguant software. The treated area was observed under microscope and histological evaluation was performed for the same. Tissue thickness was calculated morphometrically from haematoxylin and eosin stained cross sections. Reverse transcriptase PCR and immunohistochemistry were also performed to study the gene and protein level expression of angiogenic molecules. RESULTS: HRL has the ability to induce new vessel formation at the treated area and growth of the newly formed vessels and cellular morphological changes occur in a dose dependent manner. Increase in the tissue thickness at the treated area is suggestive of initiation of new capillary like structures. Elevated mRNA and protein level expression of VEGF165 and MMP2 along with the activation of ECs as demonstrated by the presence of CD34 expression supports the neovascularization potential of HRL. CONCLUSION: Angiogenic potential of HRL depends on the concentration and time of incubation and is involved in the activation of ECs along with the major interaction of VEGF 165 and MMP2. It is also observed that 3 μg of HRL exhibits maximum angiogenic potential at 72 h of incubation. Thus our data suggest that dose dependent angiogenic potential HRL could provide a novel role in angiogenic dependent therapeutics such as ischemia and wound healing conditions
Adaptive Intrusion Detection in Edge Computing using Cerebellar Model Articulation Controller and Spline Fit
No full textInternet-of-Thing (IoT) faces various security attacks. Different solutions exist to mitigate the intrusion problems. However, the existing solutions lack behind in dealing with heterogeneity of attack sources and features. The future anticipated demand of devices' connections also urge the need of new solutions addressing the concerns of time consumption and complexity. In this article, we show a novel solution for the intrusion detection in IoT framework. We configure the intrusion detection in the edge computing layer so that the effect of the attack is not propagated to the clouds. Our solution uses cerebellar model articulation controller with kernel map. This combination is very new in the direction of intrusion detection; hence, it emphasizes the novelty of our proposed intrusion detection solution. We name our solution as Cerebellar Model Articulation Controller based Intrusion Detection System (CMACIDS). Additionally, we use spline fitting to the kernel mapping for the model fit; this adds on another novel contribution to CMACIDS. The results obtained with our detection system are compared with the state-of-the-art solutions in terms of complexity, false alarms, and precision of detection. The analysis of the comparative study proves the efficiency of the solution and makes CMACIDS suitable for IoT paradigm. </p
Savaş'ın Yıkıcı Etkilerinin Ve Tecavüz Olgusunun Matei Visniec'in ''Savaş Ve Kadın'' Oyununda İncelenmesi Ve Reji Uygulaması
No full textThis art study report aims to draw attention to the horrors and atrocities of the never ending wars in human history, particularly to the status of women who were exposed to the destructive violence of war. This study evaluates the violence towards women in wars through the scope of the play ‘’War and Woman’’ by Romanian playwright Matei Visniec who analyses the genocide in Bosnia. In this play, the author has written the painful effects and destructive nature of war of this play based on a recent historical event. In the play, Visniec criticizes the 21st century civilization and questions the reason of human existence.
The study completes an application of directing with the best dose of following of the methods of contemporary theatre practitioners, by showing both open and closed characteristic of form of dramatic and narrative sections as well as of epic dramaturgy and stage-setting along with conventional-realistic-psychologic theatric dramaturgy and its stage-setting.İÇİNDEKİLER
KABUL VE ONAY………………………………………………………………….. i
BİLDİRİM…………………………………………………………………………… ii
YAYIMLAMA VE FİKRİ MÜLKİYET HAKLARI BEYANI…………………....... iii
ETİK BEYAN.................................................................................................... iv
TEŞEKKÜR.......................................................................................................v
ÖZET………………………………………………………………………………......vi
ABSTRACT…………………………………………………………………………...vii
İÇİNDEKİLER………………………………………………………………………...viii
GİRİŞ…………………………………………………………………………………..1
1. BÖLÜM:
1. YAZARIN YAŞAMI, SANATÇI KİMLİĞİ, SANATINA YÖN VEREN TARİHSEL KOŞULLAR VE ESERLERİ.............................………………………6
1.1. Yazarın Yaşamı ……………………………………………………….…6
1.2. Sanatçı Kimliği ………………………………………………………….
1.3. Yazarın Yaşadığı Dönemin Tarihsel ve Çevresel Koşulları…… 11
1.3.1. Nikolay Çavuşesku (Nicolae Ceauşescu) ve Romanya
Devrimi ……………………………………………………… 12
1.4. Yazarın Diğer Eserleri ve Oyunları …………………………………15
1.4.1. Şair Visniec’ten Oyun Yazarı Visniec’e ............................ 15
1.5. Matei Visniec Oyunlarında Genel Dramaturji Stratejisi ……… 26
1.5.1. Matei Visniec ve Postmodernizm ………………………… 29
1.5.2. Visniec’in Karakterlerinin Kaderi Hakkında ………………30
1.5.3. Matei Visniec’in Eserlerinde Tiyatronun ve İnsanın
Yabancılaşması Hakkında…………………………………..31
1.5.4. Matei Visniec’in Yazarlığı Hakkında..................................32
2. BÖLÜM:
‘’SAVAŞ VE KADIN’’ OYUNUNUN DRAMATURGİSİ ………………………… 33
2.1. Oyunun Yazılış Koşulları …………………………………………….33
2.1.1. Bosna Savaşı ………………………………………………. 33
2.1.2. Savaşın Başlangıcı…………………………………….….. 33
2.1.3. Bosna – Hersek Federasyonunun Kurulması ………… 35
2.2. Oyunun Öz, Biçim ve Biçem Özellikleri …………………………. 36
2.2.1. Oyunun Özüne İlişkin Özellikler ………………………… 36
2.2.1.1. Konu …………………………………………..…….36
2.2.1.2. Özet …………………………………………………37
2.2.1.3. Tema ………………………………………………..38
2.2.1.3.1. Bir Kavram ve Olgu Olarak Şiddet....................38
2.2.1.3.2. Şiddet Türleri……………………………………...44
2.2.1.3.3. Bir Silah Olarak Tecavüz ve Savaşlarda Kadına
Yönelen Şiddet .................................................45
2.2.1.3.4. Savaşta Tecavüz Politikası…………....…………49
2.2.1.4. Kişiler ……………………………………………….. 53
2.2.1.5. Tema Bağlamında Kişiler Arası İlişkiler ………….54
2.2.2. Oyunun Biçim Özellikleri ................................................. 56
2.2.2.1. Dramatik Biçim (Tür) ……………………………… 56
2.2.2.2. Uzam ve Zaman ……………………………………57
2.2.2.3. Kişileştirme ………………………………………….58
2.2.2.4. Eylem ………………………………………………..60
2.2.2.5. Olay Dizini ………………………………………......60
2.2.2.6. Olay Örgüsü ………………………………………...68
2.2.3. Oyunun Biçem Özellikleri …………………………………..68
3. BÖLÜM:
REJİ DEFTERİ ………………………………………………………………………73
3.1. Dramaturji Yorumu …………………………………………………....73
3.2. Seçilen Temanın Oyunculuk Yorumu ve Sahneleme İçindeki
Karşılığı ..........................................................................................76
3.3. Reji Yorumu ...................................................................................77
3.4. Işık Yorumu ....................................................................................79
3.5. Dekor, Aksesuar ve Görsel Öğelerin Yorumu.................................80
3.6. Kostüm Yorumu ..............................................................................80
3.7. Mekan ve Alan Kullanımı ................................................................82
3.8. Reji Defteri Orta Bölüm (Teknik Etmenler).......................................82
4. BÖLÜM
SONUÇ ……………………………………………………………………………..115
KAYNAKÇA………………………………………………………………………...116
EKLER ………………………………………………………………………………121
Ek 1. Orijinal Metin ........................................................................................121
Ek 2. Afiş.........................................................................................................200
Ek 3. Turnitin RaporuBu sanat çalışması raporu, insanlık tarihinde hiç bitmek bilmeyen savaşların dehşet ve vahşetine dikkat çekmeyi, özellikle savaşın yıkıcı şiddetine maruz kalan kadınların savaştaki durumunu anlatmayı amaçlamıştır. Bu çalışma savaşta kadına yönelen şiddeti, Bosna soykırımını inceleyen Romanyalı oyun yazarı Matei Visniec’in ‘’Savaş ve Kadın’’ oyunu üstünden değerlendiren bir çalışmadır. Yazar bu oyunda etnik (iç) savaşın acı çehresini ve yıkımını yakın tarihte yaşanmış bir olaydan örnekleyerek kaleme almıştır. Visniec oyununda 21. yüzyıl medeniyetini eleştirmiş, aynı zamanda insanın varoluş sebebini sorgulamayı hedeflemiştir.
Çalışmada, çağdaş tiyatro uygulayıcılarının yöntemleri izlenmiş, oyun dramatik ve anlatı bölümleriyle hem açık biçim hem kapalı biçim özelliği gösterdiği için, Epik dramaturgi ve sahne düzeni ile konvansiyonel-gerçekçi-psikolojik tiyatro dramaturgisi ve sahne düzeni, en iyi şekilde doze edilmeye çalışarak reji uygulaması gerçekleşmiştir
Sevim Burak Edebiyatı’nda “Kültürel Özne” ve “Öteki” Kavramının Tezahürlerinin İncelenmesi ve Bir Örnek Eserde Reji Uygulaması
No full textIn this art study report, the colourful world of Sevim Burak, one of the notable figures of
the Republican Period of Turkish Literature between 1950-1970 with her individual stories
with social background and innovative art perspective, has been mirrored, and the play
“İşte Baş İşte Gövde İşte Kanatlar” was chosen for the stage application. The way we draw
on ourselves through this study has been to follow the apparent manifestations of the
concept "other" and the culturalization of subject in Burak's implicit narration. The
unheard voices of the excluded, alienated characters in Burak`s texts are become visible
and felt in her works by breaking and fracturing of the language. Burak, who has produced
such works in which fact cannot be distinguished from dream, time and space are
obscured, and future and past are intertwined, criticizes the order in which she lives and
represents the "other" through her works. Considering the characteristics of Burak's art in
all this essence and form, we can say that understanding the language created by the author
with a free flow of consciousness is only possible through analysing the cultural metaphors
she uses in her plays and evaluating all of her works together which are reflections of her
own life. Throughout the course of our study, we aimed to bring the ignored voice of the
“other” out into the open through the author's extraordinary language and to reflect this
visibility into our performance. We have tried to analyse the author` s world which does
not reveal itself easily, working on the play “İşte Baş İşte Gövde İşte Kanatlar”, which is
the focus of our art study report, both theoretically and practically. The psychoanalytic
approach that we have adopted as a starting point in this way has helped us to find our way
through every time we are lost and supported our creativity in terms of both essence and
form.Bu sanat çalışması raporunda, yazdığı toplumsal arka planlı bireysel öyküleriyle ve
yenilikçi sanat anlayışıyla 1950-1970 yılları arasında Cumhuriyet Dönemi Türk
Edebiyatı’nın dikkat çeken isimlerinden biri olan Sevim Burak’ın renkli dünyasına ayna
tutulmuş, “İşte Baş İşte Gövde İşte Kanatlar” adlı oyunu seçilerek reji uygulaması
yapılmıştır. Bu inceleme boyunca kendimize çizdiğimiz yol ise “kültürel özne” ve “öteki”
kavramlarının Burak’ın örtük anlatımında kendini gösteren tezahürlerini takip etmek
olmuştur. Burak’ın metinlerinde yer alan dışlanmış, ötekileşmiş karakterlerin duyulmayan
sesleri, dilin kırılmasıyla, parçalanmasıyla eserlerinde görünürleşip hissedilmektedir.
Gerçeğin düşten ayırt edilemediği, zaman ve uzamın belirsizleştiği, geçmiş ve geleceğin iç
içe geçtiği eserler ortaya koyan Burak, yaşadığı düzeni böyle eleştirmiş ve ortaya çıkardığı
yapıtlarla “öteki”nin temsili olmuştur. Burak’ın sanatının tüm bu öze ve biçime ilişkin
özellikleri göz önünde bulundurulduğunda şunu söyleyebiliriz ki yazarın serbest bir bilinç
akışıyla meydana getirdiği dili anlamak, oyunlarında kullandığı kültürel metaforları
çözümlemekten ve kendi yaşamının yansımaları olan eserlerinin tümünü birlikte
değerlendirmekten geçer. Biz de çalışmamız boyunca yazarın sıra dışı dili aracılığıyla
“öteki”nin görmezden gelinen sesini açığa çıkarmayı ve bu görünürlüğü reji uygulamamıza
yansıtmayı hedefledik. Sanat çalışması raporumuzun odak noktasını oluşturan “İşte Baş
İşte Gövde İşte Kanatlar” oyununun hem teorik, hem de uygulamalı çalışmasını yaparak,
yazarın kendini kolay kolay ele vermeyen dünyasını çözümlemeye çalıştık. Bu yolda
hareket noktası olarak benimsediğimiz psikanalitik yaklaşım ise bize her
kaybolduğumuzda yolumuzu buldurmuş, hem öz hem de biçim anlamında yaratıcılığımızı
desteklemiştir
Co-creating Responsible Energy Systems
No full textEnergy system projects in countries like India are often failing. Not only because of technical or economical barriers, but mainly institutional and social issues are at the base of these failures. A co-creation, or participatory, process to align all demands and requirements of the different stakeholders is required. This paper takes evidence from literature on co-creation and energy systems and from case study research in India to help define an approach towards such a co-creation process as a use case for the application of the Responsible Innovation Systems framework. A discussion on co-creation as a solution generates a number of recommendations, after which a set of characteristics is concluded that the co-creation process of energy systems should have towards a responsible approach, so that more robust and sustainable innovations might emerge.Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Energy and IndustryDelft Centre for Entrepreneurshi
High molecular weight heparin-induced angiogenesis mainly mediated via basic fibroblast growth factor-2- an in-vivo (CAM) and in-silico analysis
No full textBackground: High-molecular weight heparin (HMWH), a molecule extensively used as an anticoagulant, shows concentration-dependent angiogenic and anti-angiogenic potential. So far, no studies have reported the interactive potential of HMWH with various pro-angiogenic growth factors under physiological conditions. Haence, we aimed to find the impact of major pro-angiogenic growth factors under HMWH induced angiogenesis. Methods: Chicken Chorioallantoic Membranes (CAMs) are incubated with various concentrations of HMWH. Semiquantitative PCR method was implemented to measure the changes in the transcription level of pro-angiogenic growth factors. The scanning electron microscopic technique is applied to find the morphological changes in CAM. Molecular docking and molecular dynamics simulation studies using NAMD and CHARMM force field discerned the heparin-binding mode with the pro-angiogenic growth factors. Results: HMWH can enhance the transcription level of major pro-angiogenic growth factors, significantly impacting FGF2 under 100 μM concentration. The in-silico analysis reveals that HMWH shows the highest binding affinity with FGF2. Further, molecular dynamics and interaction studies using 1 kDa Heparin against FGF2 showed that the former binds stably with the latter due to a strong salt bridge formation between the sulfate groups and arginine residues (ARG 119 and ARG109). Conclusion: The combined experimental and in-silico analysis results reveal that HMWH can interact with pro-angiogenic growth factors under micromolar concentration while inducing angiogenesis. This observation further supports the therapeutic benefits of HMWH as an angiogenic factor under such low concentration. This technique is used to replenish the blood supply to chronic wounds to speed healing and prevent unnecessary amputations
Recommendations for Improvement of the Building Design by Evaluating the Indoor Environmental Quality of the Pilot Floating House Project in Macabebe, the Philippines: floating house, the Philippines
No full textThis thesis aims to provide passive design recommendations to improve the design quality of low income houses in the Philippines, by evaluating the indoor environmental quality of the pilot floating house project. The pilot project is the result of researches carried out by Pieter Ham and Joran Van Schaik to find a solution for the housing backlog, poor living conditions and the seasonal natural disasters in the low lying areas of the Philippines. The thesis focuses on the indoor environmental quality, the thermal comfort of the pilot house. Prior to the field visit, the significant parameters for the in situ measurement and the comfort standards for the region are chosen by literature studies. A systematic measurement plan is made in order to perform in situ measurements and field study. The measurements are done for a period of nine days. In addition to this, one of the objectives of the research is to develop an economical measuring and remotely accessible monitoring device for the thermal comfort parameters. It is achieved by programming Raspberry Pi and DHT22 temperature and humidity sensor. Hypotheses are formulated after the field visit. Then the measured data is sorted systematically and analysed. This analysed data is further studied and the comfort performance of the house is compared with the comfort standards of the region. It is found that the performance is quite satisfactory for all the spaces in the house except the attic space, which has higher temperatures than the upper comfort level. Moreover the usability of the existing doors needs improvement in terms of controlling privacy and air velocity. For the improvements, it is envisioned to have minimal interventions to the existing design and construction. For this two options are put forward. Both the options are compared with the existing design by making DesignBuilder models. The simulation results of the models are compared to find the better performing strategy. Final design is made with the inference from the comparison of the simulation results, and visits to the local architecture. From the final design, the following design improvement recommendations are put forward for the housing type: “openings for ventilation at sleeping height in the attic floor” “openings at top of the roof to enhance stack effect” “collapsible doors with louvered shutters for providing users with more option in controlling air velocity and privacy”Finch Floating HomeArchitecture, Urbanism and Building Sciences | Building Technology | Sustainable Desig
Chirality transfer from chiral solvents and its memory in an azobenzene derivative exhibiting photo-switchable racemization
No full textThe transfer and dynamic fixation of chirality in cyclic azobenzenes using R-(+)-1-phenylethylalcohol (R-PEA) and S-(-)-1-phenylethylalcohol (S-PEA) as solvents or additives are investigated. The cyclic azobenzenes used in this study carry 1,5-dioxynaphthalene moiety as rotating unit, connected to the photoisomerizing (E-Z) azobenzene unit with spacers of varying lengths. With the suitable lengths of the spacers the molecules exhibit stable enantiomers originated from the element of planar chirality in the E form due to the stopped rotation of the rotor, while in the Z form the allowed rotation results in racemization. The CD spectra of racemic compounds in E form in chiral solvents were inert or almost negligible before irradiation, while 366 nm irradiation causing E-Z photoisomerization resulted in induction of clear CD bands. The thermal or photochemical reverse Z-E isomerization causes a change in the CD spectra to the new ones which are reasonably matching with the spectra of the pure enantiomers recorded in non-chiral solvents. The obtained new CD spectra are maintained even in a racemic solvent system attained by the dilution with an equal amount of chiral solvent of opposite stereo structure. These results indicate that the chirality is transferred from the chiral solvents or additives to the racemizing Z form of cyclic azobenzene and it is fixed in the non-racemizing E form. The molecule without racemization in both E and Z forms did not show any significant induced CD bands irrespective of E-Z isomerizations. The molecule showing racemization in E and Z form just show the non fixed induced CD. The property of the photo-switchable racemization is necessary for the effective transfer and temporal fixation of the chirality in this type of chirality sensors
