1,721,059 research outputs found
Analysis of the quench propagation along NbSn Rutherford cables with the THELMA code. Part I: geometric and thermal models
No full textThe paper describes the new lumped thermal model recently implemented in THELMA code for the coupled electromagnetic–thermal analysis of superconducting cables. A new geometrical model is also presented, which describes the Rutherford cables used for the accelerator magnets. A first validation of these models has been given by the analysis of the quench longitudinal propagation velocity in the NbSn prototype coil SMC3, built and tested in the frame of the EUCARD project for the development of high field magnets for LHC machine. This paper shows in detail the models, while their application to the quench propagation analysis is presented in a companion paper
Use of the automated ribotyping for epidemiological investigations
No full textIn order to understand the epidemiology of food related pathogenic bacteria, such as Salmonella spp. and Listeria monocytogenes, reproducible typing methods, which can distinguish individual strains, different in virulence and host range, are necessary. In comparison with the phenotypic techniques, many molecular typing methods offer the advantages of high discriminating ability and typeability; however a lack of standardisation and the use of different codes, within different laboratories, to identify specific genetic types, restrict their large diffusion. Both these limitations can be easily overcome using an automated technique, such as the RiboPrinter® Microbial Characterisation System (Qualicon, Inc.), which automates and standardises all process steps, from cell lysis to image analysis. In this paper examples of application of the RiboPrinter® to characterise Salmonella enteritidis and Salmonella typhimurium, as well as L. monocytogenes strains, are described. The automated ribotyping analysis seems to provide a useful tool to address the identification of the sources and the transmission routes of these foods related pathogenic bacteria
Selection and improving of fit-for-purpose sampling procedures for specific foods and risks
No full textThe European Union granted the multiyear collaborative project BASELINE (www.baselineeurope.eu) to analyze and develop sampling strategies to support the European policies in food safety with the final goal of improved quantitative risk analysis. This special issue presents the main results of the BASELINE project, particularly those related to the impact of novel concepts such as Performance Objectives (POs) and Food Safety (FSOs) in sampling plans and food safety, as well as to the validation and harmonization of alternative molecular methods for detection of main foodborne pathogens such as Salmonella spp. and Listeria monocytogenes
Effects of Dietary Vitamin E on the Quality of Table Eggs Enriched with n-3 Long-Chain Fatty Acids
No full textBecause of the proposed cardioprotective benefits of n-3 fatty acids and vitamin E, a trial was carried out to investigate the possibility of enriching eggs with n-3 fatty acid and vitamin E added to the hen's diet. One hundred ninety-two Hy-Line Brown hens, 39-wk-old, were divided into eight groups: four groups received the basal diet supplemented with 3% lard and four doses of dl-α-tocopheryl acetate (0,50, 100, and 200 ppm), whereas the diets of the other groups were supplemented with 3% of fish oil and the same doses of vitamin E. The performances of the hens and egg weights were not affected either by the type of lipid supplement or by the vitamin level. The treatment with fish oil caused a dramatic increase (P < 0.01) of all n-3 fatty acids of the yolk, particularly EPA (19.53 vs. 0.74 mg/egg) and DHA (143.70 vs. 43.66 mg/egg), and an appreciable decrease of arachidonic acid (25.54 vs. 67.72 mg/egg). The different levels of dietary vitamin E slightly affected the fatty acid composition of the yolk. Yolk α-tocopherol increased linearly as dietary dl-α-tocopheryl acetate increased (P < 0.01) from the control level of 90.93 μg/g of yolk to 313.84 μg/ g of yolk when 200 ppm were added to the hen diets. Twenty-eight days of storage at room temperature (20 to 25 C) did not alter the yolk fatty acid profile, and, moreover, the levels of vitamin E remained still very close to those observed in fresh egg
Influence of supplemental dietary taurine on antibody production in broiler chicks
No full textThe objective of the present study was to investigatge the effects of supplemental dietary taurine on antibody production in broiler chicks. The result showed that the supplemetary dietary taurine, added in a dose of 0.6% but not of 0.3% exerted a positive influence on chicken antibody production to NDV an Pa
Adjuvant properties of different forms of vitamin a in avian vaccines
No full textThe authors seek to verify previous data and to evaluate the effect of other forms of Vitamin A on chick immune response to viral and bacterial antigens. As in previous results, the data confirm that the influence on immune response of retinyl propionate vaccines is similar and retinyl acetate is less effective than normal vaccines. Moreover our results suggest that the adjuvant effect of Vitamin A, as retinyl palmitate in emulsified vaccines tends toward an increased humoral immune response with regard to NDV but not to Pateurella anatipestife
Immune response of chickens supplemented with vitamin E in diet and/or vaccine
No full textThe aim of this research was to verify if there is a synergic effect of vitam E supplid both in the diet and /or emulsified vaccine on immune response to viral and bacterial antigens. The results confirm the enhancing effect of vitamin E on the immune response to viral antigen when Vitamin E replaced light minereal oil in emulsified vaccines. On the other hand no synergic effect was observed when vitamin E was added both to vaccines and die
Ribotyping characterisation of Campylobacter isolates randomly collected from different sources in Italy
No full textIn this study the potential for using the automated PstI ribotyping as a primary library typing method to survey Campylobacter and for identification of two thermophilic Campylobacter jejuni and Campylobacter coli species was evaluated. A total of 158 isolates randomly collected in Italy from different sources were analyzed. A large percentage of chicken (28%), turkey (27%) and turkey meat (25%) isolates shared their ribotyping profiles (ribotypes) with those of humans, whereas the swine isolates had unique profiles. The identification results obtained by ribotyping corresponded to those collected by using a multiplex PCR protocol specifically designed for C. jejuni and C. coli detection. The comparison of the PstI ribotyping profiles obtained in this research with those of the isolates collected over time will facilitate determining the ribotypes that are more frequently transmitted to humans in comparison to those that are normally harboured only in animals, foods and in the environment. © 2003 Elsevier Inc. All rights reserved
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