101,658 research outputs found
Restriction of RA9 CD8 T cell epitope to <i>Mane-A*10</i>.
No full text<p>(<b>A</b>) CD8 T cell response to influenza RA9 peptide in influenza vaccinated animal 26359 (<b>B</b>) Expansion of RA9 specific CD8 T cells. RA9-specific CD8 T cell response in fresh blood is shown in comparison to a 2 week <i>in vitro</i> expansion as described in methods. (<b>C</b>) <i>Mane-A*10</i> restriction of RA9 response. Transfected (<i>Mane-A*10 or Mane-B*02</i>) and untransfected C1R cells were pulsed with either DMSO or RA9 peptide. These C1R cells were incubated separately with <i>in vitro</i> cultured RA9-specific CD8 T cells and IFN-γ and TNF-α expression measured.</p
Mane-A*10 expression did not influence CD4+ T cell decline or plasma viral load but was associated with lower CSF viral loads.
No full text<p>Comparing longitudinal trends in CD4+ T cell decline from baseline pre-infection values and plasma SIV RNA demonstrated that <i>Mane-A*10</i> expression was not associated with either extent of CD4+ T cell decline throughout infection (A) or with altered plasma viral load set points in untreated SIV-infected macaques at any time point from primary through asymptomatic to terminal stages of (B). To determine whether <i>Mane-A*10</i> expression was associated with altered viral replication in the periphery, mean plasma viral load throughout infection was measured in untreated SIV-infected animals grouped by <i>Mane-A*10</i> status (<i>Mane-A*10</i> positive animals represented by circles, n = 11; <i>Mane-A*10</i> negative animals represented by triangles, n = 21). Plasma viral load also did not differ significantly between these groups at any time-point from day 14 post-inoculation until terminal sampling (<i>P</i>>0.05). Similarly, <i>Mane-A*10</i> expression status was not associated with extent of % CD4+ T cell decline in SIV-infected macaques, with no statistically significant difference between groups of animals composed of <i>Mane-A*10</i> positive animals (circles) versus <i>Mane-A*10</i> negative macaques (triangles). Combined, these data indicate that neither plasma viral load or CD4+ T cell loss are associated with expression of <i>Mane-A*10</i> in pigtailed macaques inoculated with SIV/17E-Fr and SIV/DeltaB670. In contrast, mean SIV RNA levels in CSF were lower in the group of <i>Mane-A*10</i> positive animals (circles) versus <i>Mane-A*10</i> negative macaques (triangles).</p
CD8 T cell response in influenza-vaccinated animals by <i>Mane-A*10</i>-RA9 tetramer.
No full text<p>(<b>A</b>) <i>Mane-A*10</i>-RA9 tetramer was used at a 1∶400 dilution on samples from either animal 45418 at influenza vaccinated time-point (day 133) or influenza naïve time-point (day 0), or from an influenza unvaccinated <i>Mane-A*10</i> negative animal. (<b>B</b>) The frequency of CD8<sup>+</sup><i>Mane-A*10</i>-RA9 tetramer positive cells were compared in all influenza vaccinated animals (n = 25) to 13 influenza naïve macaques all samples were 14 days post-final vaccination with influenza virus. All samples were background corrected and the p-value for the difference between the two groups determined using an unpaired T-test. (<b>C</b>) Thawed PBMC samples (day 0–126) or whole blood (day 126 onwards) from animals with generally robust influenza-RA9 tetramer responses on initial testing (26359, B0526, B0527, 45418, 19351 and 19341) were measured for frequency of RA9-specific CD8 T cells via tetramer staining (results are background corrected). Animals were vaccinated with influenza (either X-31 or PR8 as indicated) at day 0, 28, 56 and 119, then intravaginally challenged at day 175 (Day 0 post-challenge) with SIV<sub>mac251</sub>. All animals became SIV infected with the exception of 45418(*).</p
Letter, [Author unclear] to Paulina T. Merritt
No full textHandwritten letter to Paulina Merritt from an unknown author, October 1, 1876.
Indian Constitution act and Status of Women in Society
No full textIn this article discussed Constitution Act and Status of women in Indian society. The author also highlighted the constitutional provisions of India protecting the rights and interests of women and other measures adopted by the government to ensure women's right to equality and provide them equal opportunities to raise their status in society. The education of women in India plays a significant role in improving livings standards in the country. A higher women literacy rate improves the quality of life both at home and outside the home, by encouraging and promoting education of children, especially female children, and in reducing the infant mortality rate (T. Balasubramanian 2021). the government took great steps to bring about changes in the social, economic and political conditions of women. Even today in a country like India where customs and traditions are given more importance, these measures cannot be said to be fully implemented in the society, however, women have experienced gradual changes over the years and they have been huge more independent and aware today. The 73rd Amendment believed that women's social and economic conditions could not be improved unless they were given political power. The new Panchayati Raj was an attempt to empower women at least at the village level
The inner side of T cell lipid rafts.
No full textA key question in understanding the functional role of lipid rafts is whether lipid microdomains at the plasma membrane outer leaflet are coupled to lipid microdomains at the inner leaflet. By using a cyan-fluorescent protein (CFP) targeted to inner plasma membrane rafts of Jurkat T cells, we found that raft domains at the outer and inner leaflets are physically coupled and that this coupling requires cholesterol. Interestingly, TCR/CD3 cross-linking induces co-capping of the raft bilayer independently of cholesterol or signaling events, indicating that cholesterol-extracting drugs are unable to destroy TCR-lipid rafts interaction
Handwritten biographical information on Paulina T. McClung Merritt
No full textA handwritten biography of Paulina T. McClung Merritt by an unknown author, 1892.
Heterogeneous and tissue-specific regulation of effector T cell responses by IFN-gamma during Plasmodium berghei ANKA infection.
No full textIFN-γ and T cells are both required for the development of experimental cerebral malaria during Plasmodium berghei ANKA infection. Surprisingly, however, the role of IFN-γ in shaping the effector CD4(+) and CD8(+) T cell response during this infection has not been examined in detail. To address this, we have compared the effector T cell responses in wild-type and IFN-γ(-/-) mice during P. berghei ANKA infection. The expansion of splenic CD4(+) and CD8(+) T cells during P. berghei ANKA infection was unaffected by the absence of IFN-γ, but the contraction phase of the T cell response was significantly attenuated. Splenic T cell activation and effector function were essentially normal in IFN-γ(-/-) mice; however, the migration to, and accumulation of, effector CD4(+) and CD8(+) T cells in the lung, liver, and brain was altered in IFN-γ(-/-) mice. Interestingly, activation and accumulation of T cells in various nonlymphoid organs was differently affected by lack of IFN-γ, suggesting that IFN-γ influences T cell effector function to varying levels in different anatomical locations. Importantly, control of splenic T cell numbers during P. berghei ANKA infection depended on active IFN-γ-dependent environmental signals--leading to T cell apoptosis--rather than upon intrinsic alterations in T cell programming. To our knowledge, this is the first study to fully investigate the role of IFN-γ in modulating T cell function during P. berghei ANKA infection and reveals that IFN-γ is required for efficient contraction of the pool of activated T cells
CRH-GAM – Groupe d’archéologie médiévale
No full textDanièle Alexandre-Bidon, ingénieure d’étudesPerrine Mane, directrice de recherche au CNRS Recherches croisées sur la civilisation matérielle médiévale : vignes, raisins et vins dans l’Occident médiéval Pendant l’année universitaire, le séminaire a porté sur la consommation des raisins, mais aussi des dérivés de la vigne (vins, raisins secs, rafles, moût, sarments), un accent particulier étant porté sur les diverses associations entre vins et aliments à travers l’iconographie, mais aussi les t..
Dispelling the Myths Behind First-author Citation Counts
No full textWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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