34 research outputs found
Azcorra2023 - Fiber photometry recordings (pre-processed to get DF/F)
No full textPre-processed raw data from fiber photometry recordings of different subtypes (Vglut2+, Calb1+, Anxa1+ and Aldh1a1+ as well as DAT+) SNc dopamine neurons labelled with GCaMP6f, as used in Azcorra et al. Nat Neuro 2023. This dataset has been pre-processed to calculate DF/F from the raw data (see below for code and raw data), which are then normalized from 0 to 1 (un-normalized DF/F data can be recovered using the 'norm' value included in the dataset). This dataset also includes metadata for each recording (recording location, mouse sex...).
The code used to generate this pre-processed data from raw data is available on GitHub (https://github.com/DombeckLab/Azcorra2023/releases/tag/Azcorra2023) and Zenodo (DOI: 10.5281/zenodo.7872052, https://zenodo.org/record/7872052). The original raw data has been deposited on Zenodo (DOI: 10.5281/zenodo.7871634, https://zenodo.org/record/7871634). The code necessary to analyze this data and generate the figures shown in the manuscript is is found in that same GitHub repository as the pre-processing code above
Molecular heterogeneity in the substantia nigra: A roadmap for understanding PD motor pathophysiology
No full textAs the ability to capture single-cell expression profiles has grown in recent years, neuroscientists studying a wide gamut of brain regions have discovered remarkable heterogeneity within seemingly related populations (Saunders et al., 2018a; Zeisel et al., 2015). These “molecular subtypes” have been demonstrated even within brain nuclei expressing the same neurotransmitter (Saunders et al., 2018a; Poulin et al., 2020; Ren et al., 2019; Okaty et al., 2020). Recently, dopamine (DA) neurons of the substantia nigra pars compacta (SNc) and adjacent ventral tegmental area (VTA) have been revealed to be diverse not only when comparing between these two dopaminergic nuclei, but within them, and with the distribution of identified subtypes often agnostic to traditional neuroanatomical boundaries (Saunders et al., 2018a; Hook et al., 2018; Kramer et al., 2018; La Manno et al., 2016; Poulin et al., 2014; Tiklova et al., 2019; Poulin et al., 2018). Such molecularly defined subpopulations have been the subject of several recent studies. Investigations of these subtypes have ultimately unveiled many distinctive properties across several domains, such as their axonal projections and functional properties (Poulin et al., 2018; Wu et al., 2019; Pereira Luppi et al., 2021; Evans et al., 2017; Evans et al., 2020). These key differences between subtypes have begun to corroborate the biological relevance of DA neuron taxonomic schemes. We hypothesize that these putative molecular subtypes, with their distinctive circuits, could shed light on the wide variety of dopamine-related symptoms observed across several diseases including depression, chronic pain, addiction, and Parkinson’s Disease. While it is difficult to reconcile how a single neurotransmitter can be involved in so many seemingly unrelated phenotypes, one solution could be the existence of several individual dopaminergic pathways serving different functions, with molecular subtypes serving as distinct nodes for these pathways. Indeed, this conceptual framework is already the dogma for anatomically distinct DA pathways, including the mesocortical, mesolimbic and mesostriatal pathways (Bjorklund & Dunnett, 2007). Here, we discuss our existing knowledge of DA neuron subtypes and attempt to provide a roadmap for how their distinctive properties can provide novel insights into the motor symptoms of Parkinson's disease (PD) (Fig. 1A). By exploring the differences between molecular subtypes and correlating this to their relative degeneration within the SNc, we may gain a deeper understanding of the cell-intrinsic mechanisms underlying why some DA neurons degenerate more than others in PD. Similarly, by mapping the inputs, projections, and functions of individual subtypes, we may better understand their individual roles in the circuit-level dysfunction of dopaminergic diseases
Author Correction: Unsupervised Scalable Statistical Method for Identifying Influential Users in Online Social Networks (Scientific Reports, (2018), 8, 1, (6955), 10.1038/s41598-018-24874-2)
No full textThe original version of this Article contained an error in Affiliation 3, which was incorrectly given as ‘Department of Mathematical Sciences, Universidad EAFIT, Universidad Nacional de Colombia, Medellín, Colombia’. The correct affiliation is listed below: Department of Mathematical Sciences, Universidad EAFIT, Medellín, Colombia This error has now been corrected in the HTML and PDF versions of the Article and in the accompanying Supplementary Material file. © 2019, The Author(s)
Beyond the Anthropocene: Multispecies Encounters in Contemporary Latin American Literature, Art, and Film
No full textabstract: In the face of what many scientists and cultural theorists are calling the Anthropocene, a new era characterized by catastrophic human impact on the planet’s geologic, atmospheric, and ecological makeup, Latin American writers, artists, and filmmakers today from various disciplinary and geographical positionalities are engaging in debates about how to respond ethically to this global crisis. From an interdisciplinary perspective that incorporates cutting-edge theories in multispecies ethnography, material ecocriticism, and queer ecology, this study examines multispecies relationships unfolding in three telescoping dimensions—corporealities, companions, and communities—in contemporary Latin American cultural production while uncovering indigenous and other-than-dominant epistemologies about human-nonhuman entanglements. I argue that contemporary cultural expression uncovers long, overlapping histories of social and environmental exploitation and resistance while casting the moment of encounter between individuals of different species as hopeful figurations of human-nonhuman flourishing beyond the Anthropocene. Instead of remaining hopelessly mired in the dire geographies of planetary decline, the works of Uruguayan writer Teresa Porzecanski, Mexican author Daniela Tarazona, Mexican textile sculptor Alejandra Zermeño, Argentine filmmaker Lucía Puenzo, Colombian installation artist María Fernanda Cardoso, Colombian poet Juan Carlos Galeano, Colombian graphic artist Solmi Angarita, and Brazilian poet Astrid Cabral dramatize a multitude of multispecies encounters to imagine the possibility of a better world—one that is already as close as our skin and as present as the nonhuman “others” that constitute our existence. These works imagine the human itself as a product of multispecies interactions through evolutionary time, multispecies companionships as formed around queer kinships, and biocultural communities as emerging through communicative, ethical encounters.Dissertation/ThesisDoctoral Dissertation Spanish 201
El esoterismo en la cultura europea: el tetraedro del conocimiento en las novelas de Javier Sierra
No full textabstract: This dissertation focuses on the study of Western esotericism in European culture and forms a method of discovering esoteric topics in cultural artifacts. Using the definition as a corpus of knowledge historically divided between esoteric, reserved for the intellectual and power elite, and exoteric, available for everybody, I argue that esotericism represents the knowledge that always accompanied the cultural production of the Mediterranean zone, adding a spiritual meaning to any visual or written work of art. The contemporary novels of the past decade by the Spanish author Javier Sierra are fully based on a historical investigation, in which esotericism appears as a nuclear topic, revealing the great interest of the public in the mysteries of the past. Through the postmodern cultural theories, together with sociological and historical methods, the dissertation explores the cultural processes that lead to the shift of esoteric knowledge in the 20th century from secretive to publically available. The study defines the purpose of recreating the European past and investigates the secrets of European cultural formation. Through an insider-outsider perspective, it analyzes the cultural artifacts, that appear in the novels in the form of reference or as a nuclear part of the plot. It presents the scope of esoteric currents, that are divided between the discipline of religion, science, and philosophy, which form the tetrahedron of knowledge as a theoretic model for this study. The constructed model reveals the interaction of the three disciplines throughout the history and examines the reasons for the religious disenchantment of the 20th century, proven through Digital Humanities’ research as the predominance of science over the Catholic Church, which allowed the esoteric knowledge to reappear. The study explores the affiliation of esotericism with science through the scientific-cultural inquiries between the ancient myths and reality, by showing that man’s consciousness had always been dependent on the scientific perception of the world. It explores the pagan symbolism that is mixed with Christian traditions and reveals the stories, hidden behind the representation of the greatest works of art, by combining and analyzing the wisdom of the past and the contemporary spiritual inquiries in their philosophical meaning.Dissertation/ThesisDoctoral Dissertation Spanish 201
Pólvora, sangre y sexo: dialogismos contemporáneos entre la literatura y el cine en América Latina
No full textabstract: The nature of the link between a literary text and its film adaptation has been a point of contention within academic thought since the inception of cinema due to the fact that film adaptation forms part of film history since the early 20th century. For most of the past century, the main concern of critics has been the level of fidelity that adaptations exhibit in terms of their relationship with the text, which was viewed as "the original" that directors needed to use as a model. In the last 25 years, however, the discourse of fidelity has been challenged by a number of intellectuals as a result of poststructuralist thought, which rejects the notion of an "original" text and proclaims the existence of infinite meanings within each text that are constructed by the reader, not the writer. The present investigation will take into account this type of epistemology as its starting point in order to review and defy a number of theoretical approximations from the last several decades that deal with the relationship between literature and cinema towards its main goal of overcoming the limitations of fidelity discourse. This will be carried out through an in-depth analysis of Latin American texts that have been adapted to film. Thematically both the literary texts and the films contain elements that portray the reality of marginalized groups that build their existence in opposition to the model of patriarchal heteronormativity. In current epistemological thought such a modus vivendi falls within the realm of queer theory. Another common thread that unites all the cultural productions is the presence of violence that showcases the high level of intolerance towards any subject who somehow seems to be different, hence threatening the dominant configuration of patriarchy. Furthermore, the different texts and films expose a general fragmentation within Latin American society, a result of the constant struggles among its diverse social groups, between the ones who occupy the position of socioeconomic power and those who are left outside of it; such a fragmentation also stems from the multiple clashes that occur within the marginalized groups themselves.Dissertation/ThesisPh.D. Spanish 201
Desde ángulos imposibles hacia ángulos estratégicos: narrativas de la muerte, la vida y la discapacidad en La muerte me da y El huésped
No full textabstract: From Impossible Angles Towards Strategic Ones: Narratives of Death, Life, and Disability in La Muerte me Da and El Huesped The glamour of single-handedly overcoming adversity, sidestepping obstacles, or defying the odds makes for great mystery or adventure fiction, but fails to do justice (poetic or otherwise) to lives that are both physically and conceptually "marked" by more complex challenges. From a theoretical view, a similar desire to escape or maintain the perceived "dividing line" between fact and fiction, nature and nurture, mind and body, is confronted by a diverse set of human experiences, all of which have come to be defined, and continue to define themselves, along both sides of such a divide. Disability, typically viewed as an "emerging" branch of literary and cultural critique, is perhaps the most pervasive. Hidden under the covert language of the "grotesque", "monstrous", "doppelgänger", "freak", "eccentric" or "queer", disability has historically represented something other than itself. Two texts that attest to both the real and imagined possibilities of resignification and new modes of articulation surrounding disability are La muerte me da (2007) by Cristina Rivera Garza and El huésped (2006) by Guadalupe Nettel. From different points of departure, both texts offer a narrative approximation towards the disabled mind, body, and perceptual experience. In ways that are both similar and different, these narratives question one's perceived access to that which is otherwise understood to be the physically and conceptually "inaccessible" or "illegible" space of disability. Such approximations towards, and articulations of, the disability experience are processes that move, largely unnoticed, both within and beyond texts. As this construct continues to transform itself from both within and outside itself, disability acquires intellectual and practical value while requiring the "experts" in fields beyond the narrow scope of medicine, education, and rehabilitation to (re)consider their own approaches to, and apprehensions of, disability in order to redefine what or who is accessible or viable for literary and cultural debate.Dissertation/ThesisM.A. Spanish 201
Siguiendo Las Huellas De La Chola En Bolivia: Levantamiento De Una Cartografía Cultural Alteña
No full textabstract: The surge of the chola alteña in Bolivia as a woman who, after being historically discriminated, has achieved her empowerment through her practices of resistance and agency is a very particular and new phenomenon hardly studied. The contribution of this research is in principle to describe and discover the complexity of this occurrence, but at the same time to open a field of understanding the works of the chola as a preliminary input for alternative feminisms, in accordance to the particularity of each context. As a result, an eclectic perspective from different non-canonical theories stemming from the Americas has been adopted. For example, intersectionality stemming from various social, cultural, racial, and gender contexts is addressed by Kimberlé Crenshaw, Dora Inés Munévar, Ann Phoenix, Breny Mendoza y Sonia Montecinos. Research from Aníbal Quijano, Walter Mignolo and María Lugones proposes the decolonization of knowledge. From a Bolivian perspective, the proposal of communitarian feminism by Julieta Paredes and the chi’xi approach by Silvia Rivera Cusicanqui. At the same time, the documenting of the chola practices has been obtained from non-conventional digital and oral sources. Thus, this research becomes a referent for future feminist research about the chola, but also for understanding other movements and practices of subaltern and discriminated women in similar or different contexts.
The chola is characterized by her peculiar garment which was imposed by the colonizer in the XVIII century, nullifying her indigenous identity. However, this woman has continued to wear it to the present day as much as a tactic of resistance as of empowerment and agency and has transformed it into a current fashion for the valorization of her identity. She is a chi’xi subject who complements or antagonizes opposites without subsuming them. Finally, what guides her practices and strategies are her native cultural values, such as the principle of Living Well, cooperation, reciprocity, and godfatherhood.
.Dissertation/ThesisDoctoral Dissertation Spanish 201
