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Ex vivo expansion of primary cells from limb tissue of Pleurodeles waltl
Get PDFNagasaki University (長崎大学)博士(医学)Pleurodeles waltl is coming to light as a model animal, especially in regeneration studies, but deep studies on the molecular mechanisms have been limited due to the absence of primary tissue cells for wide usage. Therefore, we aimed to grow primary cells from limb tissue of P. waltl for in vitro experiments. Limb tissues were cut into small pieces and seeded as “explants” on culture dishes coated with fibronectin and gelatin. Compared to the control without coating, both fibronectin and gelatin supported quicker outgrowth of cells from explants and faster cell adhesion, and fibronectin showed significantly better performance than gelatin. Interestingly, the doubling time of cells on fibronectin- and gelatin-coated surfaces was almost the same (42.39 ± 2.79 h vs. 42.91 ± 3.69 h) and was not significantly different from that on non-coated plates (49.64 ± 3.63 h). The cryopreserved cells were successfully recovered and showed a multiplication capacity that was similar to that of fresh cells. Senescent cells were barely detected even after long-term sub-culture (>15 passages). Moreover, enhanced fluorescence of MitoSOX™ Red in cells under H2O2 exposure confirmed the respondence to chemical stimuli. Collectively, our results show that we are able to grow enough good-quality cells from P. waltl limb tissue for in vitro experiments, and fibronectin coating provides the best biocompatible environment for cell outgrowth and attachment.長崎大学学位論文 学位記番号:博(医歯薬)甲第1702号 学位授与年月日:令和7年3月19日Author: Md. Mahmudul Hasan, Reiko Sekiya, Tao-Sheng LiCitation: Development, Growth & Differentiation, 65(5), pp.255-265; 2023Nagasaki University (長崎大学), 博士(医学) (2025-03-19)doctoral thesi
Mahmudul Hasan, Mitsuru Kuramoto, Mohammed Mafizul Islam, Mohammad Shafiqul Alam, Md Mukhlesur Rahman Khan & Masayuki Sumida (2012) A new species of genus Hoplobatrachus (Anura, Dicroglossidae) from the coastal belt of Bangladesh. Zootaxa, 3312, 45-48.
No full textHASAN, MAHMUDUL, KURAMOTO, MITSURU, ISLAM, MOHAMMED MAFIZUL, ALAM, MOHAMMAD SHAFIQUL, KHAN, MD. MUKHLESUR RAHMAN, SUMIDA, MASAYUKI (2012): Mahmudul Hasan, Mitsuru Kuramoto, Mohammed Mafizul Islam, Mohammad Shafiqul Alam, Md Mukhlesur Rahman Khan & Masayuki Sumida (2012) A new species of genus Hoplobatrachus (Anura, Dicroglossidae) from the coastal belt of Bangladesh. Zootaxa, 3312, 45-48. Zootaxa 3354 (1): 88, DOI: 10.11646/zootaxa.3544.1.8, URL: http://dx.doi.org/10.11646/zootaxa.3544.1.
sj-pdf-5-BBI-10.1177_11779322211046729 – Supplemental material for In Silico Investigation of Conserved miRNAs and Their Targets From the Expressed Sequence Tags in Neospora Caninum Genome
No full textSupplemental material, sj-pdf-5-BBI-10.1177_11779322211046729 for In Silico Investigation of Conserved miRNAs and Their Targets From the Expressed Sequence Tags in Neospora Caninum Genome by Moumita Das, Mahmudul Hasan, Sharmin Akter, Sawrab Roy, Binayok Sharma, Md. Shahidur Rahman Chowdhury, Md. Irtija Ahsan, Rubaiat Nazneen Akhand, Md Bashir Uddin and Syed Sayeem Uddin Ahmed in Bioinformatics and Biology Insights</p
sj-pdf-1-BBI-10.1177_11779322211046729 – Supplemental material for In Silico Investigation of Conserved miRNAs and Their Targets From the Expressed Sequence Tags in Neospora Caninum Genome
No full textSupplemental material, sj-pdf-1-BBI-10.1177_11779322211046729 for In Silico Investigation of Conserved miRNAs and Their Targets From the Expressed Sequence Tags in Neospora Caninum Genome by Moumita Das, Mahmudul Hasan, Sharmin Akter, Sawrab Roy, Binayok Sharma, Md. Shahidur Rahman Chowdhury, Md. Irtija Ahsan, Rubaiat Nazneen Akhand, Md Bashir Uddin and Syed Sayeem Uddin Ahmed in Bioinformatics and Biology Insights</p
sj-pdf-4-BBI-10.1177_11779322211046729 – Supplemental material for In Silico Investigation of Conserved miRNAs and Their Targets From the Expressed Sequence Tags in Neospora Caninum Genome
No full textSupplemental material, sj-pdf-4-BBI-10.1177_11779322211046729 for In Silico Investigation of Conserved miRNAs and Their Targets From the Expressed Sequence Tags in Neospora Caninum Genome by Moumita Das, Mahmudul Hasan, Sharmin Akter, Sawrab Roy, Binayok Sharma, Md. Shahidur Rahman Chowdhury, Md. Irtija Ahsan, Rubaiat Nazneen Akhand, Md Bashir Uddin and Syed Sayeem Uddin Ahmed in Bioinformatics and Biology Insights</p
sj-pdf-2-BBI-10.1177_11779322211046729 – Supplemental material for In Silico Investigation of Conserved miRNAs and Their Targets From the Expressed Sequence Tags in Neospora Caninum Genome
No full textSupplemental material, sj-pdf-2-BBI-10.1177_11779322211046729 for In Silico Investigation of Conserved miRNAs and Their Targets From the Expressed Sequence Tags in Neospora Caninum Genome by Moumita Das, Mahmudul Hasan, Sharmin Akter, Sawrab Roy, Binayok Sharma, Md. Shahidur Rahman Chowdhury, Md. Irtija Ahsan, Rubaiat Nazneen Akhand, Md Bashir Uddin and Syed Sayeem Uddin Ahmed in Bioinformatics and Biology Insights</p
sj-pdf-3-BBI-10.1177_11779322211046729 – Supplemental material for In Silico Investigation of Conserved miRNAs and Their Targets From the Expressed Sequence Tags in Neospora Caninum Genome
No full textSupplemental material, sj-pdf-3-BBI-10.1177_11779322211046729 for In Silico Investigation of Conserved miRNAs and Their Targets From the Expressed Sequence Tags in Neospora Caninum Genome by Moumita Das, Mahmudul Hasan, Sharmin Akter, Sawrab Roy, Binayok Sharma, Md. Shahidur Rahman Chowdhury, Md. Irtija Ahsan, Rubaiat Nazneen Akhand, Md Bashir Uddin and Syed Sayeem Uddin Ahmed in Bioinformatics and Biology Insights</p
sj-pdf-6-BBI-10.1177_11779322211046729 – Supplemental material for In Silico Investigation of Conserved miRNAs and Their Targets From the Expressed Sequence Tags in Neospora Caninum Genome
No full textSupplemental material, sj-pdf-6-BBI-10.1177_11779322211046729 for In Silico Investigation of Conserved miRNAs and Their Targets From the Expressed Sequence Tags in Neospora Caninum Genome by Moumita Das, Mahmudul Hasan, Sharmin Akter, Sawrab Roy, Binayok Sharma, Md. Shahidur Rahman Chowdhury, Md. Irtija Ahsan, Rubaiat Nazneen Akhand, Md Bashir Uddin and Syed Sayeem Uddin Ahmed in Bioinformatics and Biology Insights</p
sj-pdf-7-BBI-10.1177_11779322211046729 – Supplemental material for In Silico Investigation of Conserved miRNAs and Their Targets From the Expressed Sequence Tags in Neospora Caninum Genome
No full textSupplemental material, sj-pdf-7-BBI-10.1177_11779322211046729 for In Silico Investigation of Conserved miRNAs and Their Targets From the Expressed Sequence Tags in Neospora Caninum Genome by Moumita Das, Mahmudul Hasan, Sharmin Akter, Sawrab Roy, Binayok Sharma, Md. Shahidur Rahman Chowdhury, Md. Irtija Ahsan, Rubaiat Nazneen Akhand, Md Bashir Uddin and Syed Sayeem Uddin Ahmed in Bioinformatics and Biology Insights</p
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