5 research outputs found
Effect of pigments of Pseudomonas aeruginosa on adhering and cytotoxicity of A549 cell line
No full textPseudomonas aeruginosa gram-negative, bacilli and facultative aerobic, P. aeruginosa cause cystic fibrosis patients, wounds, burns, and immunodeficienct patients, that have many virulence factors such as pyocyanin , cytotoxic ,biofilm formation and motility, Eighty-eight isolates belonging to P. aeruginosa were collected including the 66 clinical isolates obtained from different hospitals in Baghdad and were from different sources and 22 environmental isolates from previous studies of soil near oil fields. Microscopical and cultural characteristics were studied and diagnosed using biochemical tests, VITEC device, their ability to adhere to non-living (Polystyrene), living cell line (A549) and cytotoxicity of bacterial filtrate by MTT method. The results displayed that all isolates belonged to P. aeruginosa. The pigment-forming (pe26 – pc36) isolates and (PE33 – PC31) non-pigment-forming isolates were selected. That all selected bacteria were able to adhere to the Polystyrene and an epithelial carcinoma of lung (A549) was of more than 300 colony formation units in dilution (1:10) ,(1:1000), and (1:10000). The toxicity of the P. aeruginosa filtrate (pc36) isolated from clinical sources and producing pigments was 15.7, 34.5, 44 % at a concentration of 40, 60, 80 % respectively, while the isolate (pc31) that was isolated from clinical sources and non-producing pigment was 28.1, 75.2, 80.9 % at the same concentrations. As for the isolate (pe26),isolated from environmental sources and forming the pigment, the inhibition rate was 38.5, 83.1, 48.8 % at concentrations of 40, 60, 80) % respectively, and the isolate (PE33) that was isolated from environmental sources was 42, 73.4, 74.1 % at concentrations of 40, 60, 80 % respectively. The study will be helpful in evaluating the effect of pigment formation in P. aeruginosa on adhesion
The Effect of Cronobacter Sakazakii on the Gastro-Intestinal Tract of Newborn Mice
No full textFifteenth of Cronobacter sakazakii were obtained from previous studies isolates were (5 infant formula, 5 spinal fluid and 5 bloods). C. sakazakii is ability to survive on long time in environment as powder infant material which leads transmission bacteria to the immune compromised infant . Fifteen new born mice were obtained from the Iraqi Center for Cancer and Medical Genetics whose ages ranged between 10-17 days and their weights 8.5 ± 1.25 gm, were divided into 3 groups. The samples were cut and placed in formalin, then placed in stabilizer and concentrations of alcohol (70%, 80%, 90%,100%) to complete dehydration after they were leached with xylene, embedded the samples paraffin wax (58°C) and sectioning the with thickness (5) micrometer and stained with Haematoxylin and Eosin stain. The results showed that swelled of the gastro-intestinal tract and fluid accumulation was observed for the animals vaccinated with C. sakazakii an increase in the weight of the gastro-intestinal tract for the mice compared to the control treatment and this increase was significant at the probability level p≤0.05 this may be due to the occurrence of inflammatory symptoms in the affected organ 72 hours after the injury the histological study of the gastro-intestinal tract and the tunica mucosa was distinguished by deep enterocytes goblet cells and muscular was thick . It was represented by loss of villi shedding of the epithelium with rupture of the intestinal epithelial cells and their rupture in the lower of the gastrointestinal tract. Edema of the sub mucosal epithelium has been observed. Goblet cells necrosis and filter immune cells neutrophils, macrophages, eosinophil, and lymphocytes fill the lumen C. sakazakii concentration 103 and 105 cells necrotizing enterocolitis of the newborn mouse
The Eeffect of Cronobacter sakazakii on the Brains of Newborn Mice
No full textFifteenth of Cronobacter sakazakii were obtained from previous studies isolates were (infant formula, spinal fluid and bloods). The newborn mice and number was 15 obtaining healthy newborn mice from the Iraqi Center for Cancer and Medical Genetics whose ages ranged between 10-17 days and their weights 8.5 ± 1.25 gm, were divided into 3 groups. The samples were cut and placed in formalin, then placed in stabilizer and concentrations of alcohol (70%, 80%, 90%,100%) to complete dehydration after they were leached with xylene, embedded the samples paraffin wax (58°C) and sectioning the with thickness (5) micrometer and stained with Haematoxylin and Eosin stain. The results of an increase in the weight of the brain organ for the mice compared to the control treatment and this increase was significant at the probability level p≤0.05 and the results showed the inflammatory response of the brain of large number of polymorphnuclear cells and mononuclear cells as well as congested blood vessels with an increase in the size of endothelial cells. The presence of bleeding congestion and shrinking of nerve cells in the area of the cerebral cortex, gliosis (neuroma), was more prominent in the molecular layer in the brain of the newborn mice fed at a concentration of 105 cells and the occurrence of oedema ,the presence of haemorrhage in both concentrations of dosed newborn mice and necrosis of the choroid plexus concentration103 and 105 cells
Retraction notice to “Fusing network traffic features with host traffic features for an improved 5G network intrusion detection system” Optik 271 (2022) 170079
No full textThis article has been retracted: please see Elsevier Policy on Article Withdrawal (https://www.elsevier.com/locate/withdrawalpolicy). This article has been retracted at the request of the Editor-in-Chief. The journal was alerted to a PubPeer post containing a number of allegations, including that the article contains several questionable references that are likely to be unreliable and several tortured phrases that makes some passages hard to parse, as detailed here: PubPeer - Fusing network traffic features with host traffic features f. The journal requested the authors to provide an explanation of these concerns, as well as raw data and any related evidence. The corresponding author and co-author did not respond. The Editor-in-Chief assessed the case and decided to retract the article. © 2023 Elsevier Gmb
Statistical Study of Bacterial Urinary Tract Infections for the Period 2019 to 2023
No full textThe current review analyzed bacterial urinary tract infections (UTIs) in Iraq from 2019 to 2023 by highlighting the prevalence of gram-positive and gram-negative Bacteria among UTIs. Escherichia coli was the main pathogen, with 1430 bacterial isolates from UTI cases, followed by Klebsiella pneumonia with 721 isolates, and Pseudomonas sp. with 182 isolates. The current study showed that the gram-positive bacteria, represented by Staphylococcus aureus, were the dominant pathogen in UTI cases, with 229 isolates. The distribution of these pathogens varied annually, indicating changes in infection trends. The dominance of E.coli and its multi-drug resistance were consistent with global findings, confirming its clinical importance. The study highlighted other bacterial species responsible for UTIs, which were Proteus sp., Enterobacter sp., and Enterococcus sp., confirming the complexity of the pathogenesis of UTIs. This statistical study provides critical insights into bacterial epidemiology and guides future research for the management of UTIs in Iraq.
Conclusions: The present review provides a comprehensive analysis of bacterial UTIs in Iraq from 2019 to 2023, emphasizing the prevalence and distribution of pathogens. E. coli was the most commonly causative agent, followed by K. pneumonia and Pseudomonas sp., with significant multi-drug resistance patterns noted, mirroring global trends. Gram-positive bacteria, S. aureus was predominant. Variability in pathogen distribution highlights evolving infection trends and complexities in UTI pathogenesis. The findings underscore the critical need for ongoing epidemiological surveillance and antibiotic stewardship to manage UTIs effectively and mitigate resistance. This data is pivotal for improving healthcare strategies and patient outcomes in Iraq
