37 research outputs found
Supplementary_data – Supplemental material for Non-interventional study of the safety and effectiveness of fluticasone propionate/formoterol fumarate in real-world asthma management
No full textSupplemental material, Supplementary_data for Non-interventional study of the safety and effectiveness of fluticasone propionate/formoterol fumarate in real-world asthma management by Vibeke Backer, Adam Ellery, Sylvia Borzova, Stephen Lane, Magda Kleiberova, Peter Bengtsson, Tadeusz Tomala, Dominique Basset-Stheme, Carla Bennett, Dirk Lindner, Arthur Meiners and Tim Overend in Therapeutic Advances in Respiratory Disease</p
Real‐Time VCC Monitoring and Forecasting in HEK‐Cell‐Based rAAV Vector Production Using Capacitance Spectroscopy
No full textABSTRACT Recombinant adeno‐associated virus (rAAV) vector production is a complex process in which the robust cultivation of human embryonic kidney cells (HEK293) plays a critical role in generating high‐quality viral vectors. Tracking the viable cell concentration (VCC) during upstream production is essential for process monitoring and for implementing actions that ensure optimal process management. The advent of inline capacitance probes has introduced a crucial process analytical technology (PAT) tool for real‐time VCC measurement. Here, we present the development and application of a method for real‐time monitoring of VCC in HEK293‐based rAAV vector production. In a first step, BioPAT Viamass probes were used to record capacitance data of individual 10 L rAAV‐8 batches within a frequency range of 50 kHz–20 MHz. Based on the capacitance data, a linear single‐frequency model and an orthogonal partial least square (OPLS) multifrequency model for VCC prediction were developed. Subsequently, these models were deployed inline, and predictions were exposed into BioPAT MFCS bioprocess control software, enabling real‐time VCC monitoring in subsequent rAAV‐8 production batches. In addition, the continuous VCC signal was used as input for an exponential cell growth model that was deployed inline to provide accurate real‐time forecasting of the transfection time point. To the best of our knowledge, this is the first example of inline deployment of VCC and Time‐Till‐Transfection predictive models to the bioprocess control system for real‐time monitoring and forecasting of these parameters in HEK‐cell‐based transient rAAV vector production
Kantor – konteksty polskie i światowe
No full textTheatermachine Tadeusz Kantor in Context, red. Magda Romanska i Kathleen Cioffi, Northwestern University Press 202
Evolution of Slovenia's trade on an international basis after gaining independence - emphasis on trade cooperation between Czech republic and Slovenia
No full textTato bakalářská práce s zabývá vývojem mezinárodního obchodu Slovinska v kontextu ekonomiky státu, následně pak jeho analýzou. Specifikem práce je pak porovnání s Česku republikou aplikované v průběhu celé práce, zejména však ve zvláštní kapitole zaměřené na česko-slovinskou obchodní spolupráci. Závěrem práce je pak prognóza budoucího vývoje ve Slovinsku se zaměřením na mezinárodní obchod včetně vyplývajících doporučení.Broadly, this thesis focuses on analysing the evolution of Slovenian international trade in the context of state economy. Throughout the thesis, Slovenia is being compared to Czech republic. Furthermore, there is a separate chapter dealing with trade cooperation between these two countries. The thesis concludes with a prognosis of Slovenia's future evolution with a particular focus on international trade as well as with possible recommendations
Evolution of Slovenia's trade on an international basis after gaining independence - emphasis on trade cooperation between Czech republic and Slovenia
No full textBroadly, this thesis focuses on analysing the evolution of Slovenian international trade in the context of state economy. Throughout the thesis, Slovenia is being compared to Czech republic. Furthermore, there is a separate chapter dealing with trade cooperation between these two countries. The thesis concludes with a prognosis of Slovenia's future evolution with a particular focus on international trade as well as with possible recommendations
Entwicklung und Anwendung von Strategien zur Expansion pluripotenter Stammzellen
No full text[no abstract
pEDFM (Projection-based Embedded Discrete Fracture Model) on Corner Point Grid for mass transport in fractured heterogeneous porous media
No full textRegular Cartesian grid models provide satisfactory numeric results when a numerical scheme for reservoir flow simulation is applied. However, they cannot recreate complex geological features existing in realistic reservoir models such as faults and irregular reservoir boundaries. Corner point grids can represent these geological characteristics and can be adapted and represent any reservoir. In the subsurface reservoirs is usually typical to find fractures networks, and it is necessary to simulate the effect of them in reservoir models based on corner point grids. Although several works validate the precision of embedded Discrete Fracture Model (EDFM) for representing fractures in cartesian grids, very few studies have been presented to examine the accuracy of fracture modeling in geologically complex reservoir models. In this work, the novel discrete fracture model, the Projection-based Embedded Discrete Fracture Model (pEDFM), is implemented to represent fractures in reservoir models based on corner point grids. pEDFM provides additional features to the EDFM and is applied to explicitly and consistently define fractures. It implements independent grid sets for the fractures (described as lower-dimensional domains) and the rock matrix irrespective of the grid domains’ complex geometrical shapes. The suitability of the original pEDFM method has been expanded to a fully generic 3D geometry, and it lets on including fractures with any orientation on the corner point grid cells, an important development for the method’s viability in field-scale applications. Further to the geometrical flexibility of EDFM, matrix-matrix and fracture matrix connectivities are readapted to account for the projection of fracture plates on the interfaces. This allows for consistent modeling of fractures with generic conductivity values, from high conductive networks to impermeable flow barriers. A fully implicit scheme is used to get a discrete system with two main unknowns (i.e., pressure and phase saturation) on both matrix and fracture networks. Several 3D test cases of reservoirs models with complex corner point grids and fracture networks arbitrary designed in them are presented to demonstrate the devised method’s accuracy and applicability. The results show that the pEDFM implementation for two-phase flow is highly successful for modeling fractures with a broad range of conductivity on field-scale reservoir models
Projection-based Embedded Discrete Fracture Model (pEDFM) on Corner-point Grid Geometry for Subsurface Flow and Geothermal Modeling
No full textWe develop projection-based embedded discrete fracture model (pEDFM) on corner-point grids (CPG) for fluid flow and heat transfer in subsurface geological formations. The coupling between the flow and heat transfer is fully-implicit, to allow for stable simulations, specially in presence of highly contrasting fractures. We define independent CPG-based mesh for matrix rock and all 3D fractures, which allows for capturing geologically complex geometries. The connectivities between the non-neighbouring cells are described such that a consistent discrete representation of the embedded fractures are developed within the CPG geometry. Numerical rests are developed first to verify the CPG grid implementation compared with the Cartesian structured ones, and then to illustrate the applicability of the pEDFM for field-scale geologically complex reservoirs.Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Numerical AnalysisReservoir Engineerin
From Nature to Culture… and Back? On the Book <i>Biopolityka męskości</i>
No full textThe paper is a review of the co-authorship book entitled Biopolityka męskości [Biopolitics of Manhood]. The author notices that this scientific monograph locates the Polish masculinities studies on the new field of biopolitics, immunisation and tanatopolitics, giving a hope to elaborate an alternative methodology of studies on literature and culture of this range. The author appreciates researchers’ achievements and suggests a few contexts expanding their reflections. Both the prior conference and subjective publication, in author’s opinion, open the new chapter of the Polish men’s studies
Problem umocnień obronnych Oświęcimia w wyniku badań archeologicznych przeprowadzonych w 2023 i 2024 r.
No full textOświęcim first appeared in historical records in 1179. In 1284, a mayor was mentioned, which may indicate that the town already possessed town privileges. Its development accelerated when, following the division of the Cieszyn demesne, it became the seat of Prince Władysław in 1316.
Scholars speculate that Oświęcim may once have been encircled by defensive walls, though the surviving sources are scarce and ambiguous. In 1563, a special liquor excise tax was imposed, intended for the completion of the brick perimeter, and the work was still in progress in 1572. A map from 1563, Ducatus Oswieczensis et Zatoriensis, depicts Oświęcim as enclosed by walls with towers rising above them, while the gatehouses contained single-room chambers above the passageways.
Although no remains of the brick fortifications have yet been discovered, fragments of the town moat have been identified in several locations. The moat is thought to have been about 6 metres wide and 1.5 metres deep. According to A. Przybylok’s hypothetical reconstruction of the defensive perimeter, they should be expected along Sienkiewicza Street, outside the area of “our” investment. Przybylok also suggested that, in order to reduce earthworks, natural watercourses and slopes were often utilised in Upper Silesian urban centres – probably including Oświęcim – when constructing moats.
The archaeological findings raise two key questions. The first concerns the actual extent of the medieval town. The moat functioned not only as a defensive structure but also as a boundary demarcating an area governed by different laws than the surrounding non-urban land, which was likely used for agriculture, horticulture, or animal husbandry. These divisions persisted even after the moat had been filled in, as shown by the path or street marked on maps from 1845 and 1884. The second question is whether Oświęcim ever possessed a complete brick defensive circuit. The town is situated on a hill, rising more than 10 metres above the surrounding terrain. It is therefore possible that the fortifications were limited to the gateways, while the moat and the natural elevation provided sufficient protection for the inhabitants.
RECEIVED 20.05.2025 • VERIFIED15.07.2025 • ACCEPTED21.07.2025
Funding Information: Not applicable
Conflicts of interests: None
Ethical Considerations: The Authors assure of no violations of publication ethics and take full responsibility for the content of the publication.
The percentage share of the author in the preparation of the work is: JT 50%, PT 50%
Declaration regarding the use of GAI tools: not used Oświęcim pojawia się po raz pierwszy w źródłach historycznych w 1179 r. W 1284 r. wymieniono wójta, co może oznaczać, iż Oświęcim posiadał już prawa miejskie. Rozwój ośrodka przyspieszył jego awans na stolicę księcia Władysława w 1316 r., po podziale dominium cieszyńskiego.
Badacze przypuszczają, iż Oświęcim mógł być otoczony murami obronnymi. Przekazy są nieliczne i enigmatyczne. Ustanowiony specjalny podatek czopowy w 1563 r. przeznaczyć miały władze miasta na ukończenie murowanego obwodu. Prace trwały jeszcze w 1572 r. Według mapy z 1563 r. Ducatus Oswieczensis et Zatoriensis Oświęcim otaczały mury i wyższe od nich baszty, a w budynkach bramnych nad przejazdem mieściły się jednoizbowe pomieszczenia.
Relikty murowanych umocnień nie zostały dotychczasowo odkryte, za to w kilku miejscach natrafiono na fragmenty fosy miejskiej. Miała ona mieć około 6 m szerokości i 1,5 m głębokości. Według hipotetycznej rekonstrukcji przebiegu obwodu obronnego A. Przybyloka należałoby ich się spodziewać wzdłuż ul. Sienkiewicza, poza terenem „naszej” inwestycji. Badacz ten sugerował, iż przy budowie fosy, aby zminimalizować nakład prac ziemnych, wykorzystano w różnych ośrodkach miejskich Górnego Śląska naturalne cieki i skarpy, w tym również prawdopodobnie i w Oświęcimiu.
Po badaniach archeologicznych nasuwają się dwa pytania. Pierwsze – jaką faktycznie powierzchnię zajmowało średniowieczne miasto. Fosa była nie tylko obiektem obronnym, ale jednocześnie granicą miasta, gdzie obowiązywały odrębne prawa niż na obszarze pozamiejskim, który zapewne wykorzystywano pod rolnictwo lub ogrodnictwo czy hodowlę. Podziały te, nawet po zasypaniu fosy, nadal obowiązywały, o czym świadczy ścieżka czy też uliczka zaznaczona na planach z 1845 i 1884 r. Drugie – czy Oświęcim posiadał pełny murowany obwód obronny. Miasto usytuowane jest na wzgórzu, o znacznym, nawet powyżej 10 m przewyższeniu w stosunku do otaczającego terenu. Być może umocnienia obronne ograniczono tylko do przejazdów bramnych. Prawdopodobnie fosa i przewyższenie wystarczyły do zabezpieczenia mieszkańców.
RECEIVED 20.05.2025 • VERIFIED15.07.2025 • ACCEPTED21.07.2025
Funding Information: Not applicable
Conflicts of interests: None
Ethical Considerations: The Authors assure of no violations of publication ethics and take full responsibility for the content of the publication.
The percentage share of the author in the preparation of the work is: JT 50%, PT 50%
Declaration regarding the use of GAI tools: not used
