1,720,956 research outputs found
Characterization of a new Mycobacterium avium conjugative plasmid.
No full textObjective: Characterization of the pMA100 conjugative plasmid from Mycobacterium avium, identifying its replication origin and conjugation mechanism. Material and Methods: To identify and describe the main components of pMA100, assembly and annotation were carried out coupled with bioinformatics analysis and review of literature. Possible regions containing the origin of replication were identified, considering characteristics already described for replication origins of other mycobacterial plasmids, and cloned by the construction of E. coli-Mycobacterium shuttle plasmids. Six recombinant plasmids were constructed, three involving the rep1 gene and different upstream regions, and three involving the rep2 gene and different upstream regions. Rapidly growing mycobacteria (RGM) (M. smegmatis mc2155, M. abscessus ATCC 19977) and slow growing mycobacteria (SGM) (M. bovis BCG Moreau and Pasteur) were transformed with the six recombinant plasmidsconstructed. Additionally, a conjugative mechanism of pMA100 was proposed based on bioinformatics analyses and literature review of its components. Results: Bioinformatics analyses showed that the pMA100 plasmid belongs to a family of SGM plasmids which is characterized by the presence of three conserved regions, related to the conjugation mechanism of this plasmid: one secretion system type 4 (SST4) locus, one secretion system type 7 (SST7) locus and the exonuclease V gene. The existence of a functional replication origin in pMA100 was proven with two constructions involving the rep1 gene: rep1-2539 and rep1-1708. Construction rep1-2539 generated transformant colonies with M. smegmatis mc2155, M. bovis BCG Pasteur and Moreau, while construction rep1-1708 generated transformant colonies only with M. smegmatis mc2155. Conclusions: The pMA100 plasmid has a functional origin of replication in the upstream region of the rep1 gene, and this plasmid conjugation probably occurs by a novel mechanism involving components of SST4, SST7 and exonuclease V.Objetivo: Caracterização do plasmídio conjugativo pMA100 de Mycobacterium avium, identificando sua origem de replicação e mecanismo de conjugação. Material e Métodos: A montagem e anotação de pMA100 foram realizadas aliadas a uma análise bioinformática e revisão da literatura para identificar e descrever os principais componentes desse plasmidio. Possíveis regiões contendo a origem de replicação de pMA100 foram identificadas, considerando as características de origens de replicação já descritas para outros plasmídios de micobactérias, e clonadas mediante a construção de plasmídios shuttle E. coli-Mycobacterium. Seis plasmídios recombinantes foram construídos, três envolvendo o gene rep1 e diferentes regiões a montante deste gene, e outros três envolvendo o gene rep2 e diferentes regiões a montante deste gene. Essas construções foram utilizadas na transformação de micobactérias de crescimento rápido (MCR) (M. smegmatis mc2155, M. abscessus ATCC 19977) e micobatérias de crescimento lento (MCL) (M. bovis BCG Moreau e Pasteur). Adicionalmente, um mecanismo de conjugação foi proposto com base na análise bioinformática de pMA100 e revisão da literatura sobre seus componentes. Resultados: A análise bioinformática revelou que o plasmídio pMA100 pertence a um grupo de plasmídios de MCL que se caracteriza pela presença de três regiões conservadas, relacionadas ao mecanismo de conjugação deste plasmídio: um locus do sistema de secreção tipo 4 (SST4), um locus do sistema de secreção tipo 7 (SST7) e um gene de exonuclease V. A existência de uma origem de replicação de pMA100 funcional em micobactérias foi comprovada em duas construções envolvendo o gene rep1: rep1-2539 e rep1-1708. A construção rep1-2539 gerou colônias transformantes em M. smegmatis mc2155, M. bovis BCG Moreau e Pasteur, enquanto que a construção rep1-1708 gerou colônias transformantes somente em M. smegmatis mc2155. Conclusões: O plasmídio pMA100 possui uma origem de replicação funcional na região a montante do gene rep1, e o processo de conjugação deste plasmídio possivelmente ocorre por um mecanismo inédito, envolvendo componentes do SST4, SST7 e a proteína exonuclease V.Dados abertos - Sucupira - Teses e dissertações (2013 a 2016
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
Author Under Sail The Imagination of Jack London, 1893-1902
No full textIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Intro -- Title Page -- Copyright Page -- Dedication -- Contents -- Acknowledgments -- Introduction -- 1. Spirit Truth -- 2. From Absorption to Theatricality and Back Again -- 3. "I Will Build a New Present" -- 4. Sons as Authors -- 5. Fathers as Publishers -- 6. The Daughter as Author -- 7. Lovers as Authors -- 8. At Sea with the Family -- 9. Yellow News, Yellow Stories -- 10. The Return Home -- Notes -- Bibliography -- Index -- About Jay WilliamsIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Description based on publisher supplied metadata and other sources.Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, YYYY. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
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