191,556 research outputs found
Characterisation of Bombyx mori odorant-binding proteins reveals that a general odorant-binding protein discriminates between sex pheromone components
No full textIn many insect species, odorant-binding proteins (OBPs) are thought to be responsible for the transport of pheromones and other semiochemicals across the sensillum lymph to the olfactory receptors (ORs) within the antennal sensilla. In the silkworm Bombyx mori, the OBPs are subdivided into three main subfamilies; pheromone-binding proteins (PBPs), general odorant-binding proteins (GOBPs) and antennal-binding proteins (ABPs). We used the MotifSearch algorithm to search for genes encoding putative OBPs in B. mori and found 13, many fewer than are found in the genomes of fruit flies and mosquitoes. The 13 genes include seven new ABP-like OBPs as well as the previously identified PBPs (three), GOBPs (two) and ABPx. Quantitative examination of transcript levels showed that BmorPBP1, BmorGOBP1, BmorGOBP2 and BmorABPx are expressed at very high levels in the antennae and so could be involved in olfaction. A new two-phase binding assay, along with other established assays, showed that BmorPBP1, BmorPBP2, BmorGOBP2 and BmorABPx all bind to the B. mori sex pheromone component (10E,12Z)-hexadecadien-1-ol (bombykol). BmorPBP1, BmorPBP2 and BmorABPx also bind the pheromone component (10E,12Z)-hexadecadienal (bombykal) equally well, whereas BmorGOBP2 can discriminate between bombykol and bombykal. X-ray structures show that when bombykol is bound to BmorGOBP2 it adopts a different conformation from that found when it binds to BmorPBP1. Binding to BmorGOBP2 involves hydrogen bonding to Arg110 rather than to Ser56 as found for BmorPBP1
La libertà umana. Storia di una controversia filosofica
No full textSaggi di M. De Caro, L. Fonnesu, G. Lettieri, M. Mori, C. Natali, P. Porro, E. Spinelli, F. Trabatton
Marisa Mori e la libertà della pittura
No full textIntroduzione alla mostra, ideata e curata da E. Acanfora e C. Toti, dedicata alla pittrice Marisa Mori, che fu allieva di Felice Casorati e si orientò quindi al Futurismo. La mostra riscopre le opere della pittrice negli anni in cui fu legata al Lyceum Internazionale di Firenze. La scelta delle opere esposte si deve a E. Acanfora e C. Toti
L'aborto tra morale e bioetica nella riflessione di Maurizio Mori
No full textIl capitolo prensenta la riflessione di Maurzio Mori sull'aborto, come una riflessione particolarmente profonda nel contesto della bioetica italiana laica. Si considera l'atttualità della stessa e si segnano, in conclusione, alcuni pochi punti di divergenza con le tesi dell'autrice sullo stesso tema.In the chapter Maurizio Mori's reflexion on abortion is presented as one of the deepest in the contest of the italian lay bioethics. While the actuality of his analysis is underlined, a few point of disagreemt are mentioned in the end
Presentazione [al volume Jeffrey C. Alexander, Trauma. La rappresentazione sociale del dolore]
No full textPresentazione del volum
Il marketing politico (Nodo della rivista "Iride")
No full textIl nodo dedicato al marketing politico include i seguenti contributi: DONATELLA CAMPUS, "Il marketing politico: fare la politica attraverso la comunicazione"; CARLO FORMENTI: "Rivolte nordafricane e social media. Importazione della democrazia o esportazione della lotta di classe?"; DOMINIC WRING, "L'inarrestabile ascesa dei professionisti delle elezioni?"; LUCA MORI, "Il marketing politico e il consenso in democrazia"
Contemporary Art in Japan and Cuteness in Japanese Popular Culture
No full textThis thesis is an art historical study focussing on contemporary Japan, and in particular the artists Murakami TakashL Mori Mariko, Aida Makoto, and Nara Yoshitomo. These artists represent a generation of artists born in the 1960s who use popular culture to their own ends. From the seminal exhibition 'Tokyo Pop' at Hiratsuka Museum of Art in 1996 which included all four artists, to Murakami's group exhibition 'Little Boy: The Arts of Japan's Exploding Subculture' which opened in April 2005, central to my research is an exploration of contemporary art's engagement with the pervasiveness of cuteness in Japanese culture.
Including key secondary material, which recognises cuteness as not merely something trivial but involving power play and gender role issues, this thesis undertakes an interdisciplinary analysis of cuteness in contemporary Japanese popular culture, and examines howcontemporary Japanese artists have responded, providing original research through interviews with Aida Makoto, Mori Mariko and Murakami Takashi. Themes examined include the deconstruction of the high and low in contemporary art; sh6jo (girl) culture and cuteness; the relation of cuteness and the erotic; the transformation of cuteness into the grotesque; cuteness and nostalgia; and virtual cuteness in Japanese science fiction animation, and computer games.
Director of Studies: Toshio Watanabe
Supervisors: David Ryan and Omuka Toshihar
A highly efficient method for the generation of a recombinant Bombyx mori nuclear-polyhedrosis-virus Bacmid and large-scale expression of foreign proteins in silkworm (B. mori) larvae
No full textIn the post-genomic era, one of the challenges and a source of competition is the development of high-throughput, large-scale and low-cost eukaryotic cDNA cloning and expression systems. The baculovirus expression system is the most popular one and plays an important role in the high-level expression of eukaryotic proteins. In the present study, a convenient, rapid and highly efficient method for the construction of recombinant BmNPV (Bombyx mori nuclear polyhedrosis virus)-Bacmid vector (BmBacmid) for low-cost protein expression in silkworm (B. mori) larvae was established by using the MAGIC (mating-assisted genetically integrated cloning) strategy. By simply mixing the donor bacteria strain containing the constructed donor vector pCTdual harbouring foreign genes and the recipient strain containing modified BmBacmid, 99.8% positive recombinant BmNPV-Bacmids were obtained. Reporter genes egfp (enhanced green fluorescent protein gene) and DsRed (Discosoma sp. red fluorescent protein gene) and target gene man (beta-mannanase gene) encoding beta-mannanase were expressed in the silkworm larvae of B. mori at high level by injection of recombinant BmBacmid DNA directly with the standard calcium phosphate transfection procedure. The possibility of constructing a high-quality baculovirus cDNA library by transferring an ordinal plasmid cDNA library into the recipient BmBacmid in Escherichia coli was explored
- …
