1,721,057 research outputs found
Phenolic compounds from Byrsonima crassifolia L. bark: Phytochemical investigation and quantitative analysis by LC-ESI MS/MS
No full textPhytochemical investigation of the methanolic extract of Byrsonima crassifolia's bark led to the isolation of 8 known phenolic compounds 5-O-galloylquinic acid, 3-O-galloylquinic acid, 3,4-di-O-galloylquinic acid, 3,5-di-O-galloylquinic acid, 3,4,5-tri-O-galloylquinic acid, (+)-epicatechin-3-gallate along with (+)-catechin and (+)-epicatechin.Due to their biological value, in the present study, a high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method, working in multiple reaction monitoring (MRM) mode, has been developed to quantify these compounds. B. crassifolia bark resulted in a rich source of phenolic compounds and particularly of galloyl derivates. The proposed analytical method is promising to be applied to other galloyl derivatives to quantify these bioactive compounds in raw material and final products
Liquid chromatography tandem mass spectrometry determination of chemical markers and principal component analysis of Vitex agnus-castus L. fruits (Verbenaceae) and derived food supplements
No full textA validated anal. method for the quant. detn. of seven chem. markers occurring in a hydroalcoholic ext. of Vitex agnus-castus fruits by liq. chromatog. electrospray triple quadrupole tandem mass spectrometry (LC/ESI/(QqQ)MSMS) is reported. To carry out a comparative study, five com. food supplements corresponding to hydroalcoholic exts. of V. agnus-castus fruits were analyzed under the same chromatog. conditions of the crude ext. Principal component anal. (PCA), based only on the variation of the amt. of the seven chem. markers, was applied in order to find similarities between the hydroalcoholic ext. and the food supplements. A second PCA anal. was carried out considering the whole spectroscopic data deriving from liq. chromatog. electrospray linear ion trap mass spectrometry (LC/ESI/(LIT)MS) anal. High similarity between the two PCA was obsd., showing the possibility to select one of these two approaches for future applications in the field of comparative anal. of food supplements and quality control procedures
Phenylpropanoid Glycosides from Tynanthus panurensis: Characterization and LC-MS Quantitative Analysis.
No full textMedicinal plants in the treatment of women's disorders: Analytical strategies to assure quality, safety and efficacy
No full textDuring last decades an increasing number of herbal products specifically targeting women's disorders has appeared in the worldwide marketplace. This growth highlights the need for a critical evaluation of quality, safety and efficacy of these products. Analytical techniques applied to the quality control of the main medicinal plants used for women health (relief of menopause and menstrual related symptoms) have been reviewed. Thanks to the innovation in analytical technology, identification and detection of secondary metabolites dramatically improved. In particular, hyphenated techniques have proved to be the most suitable for the rapid identification of compounds in plant matrix. Moreover, taking into account that differences in sample quality are not only found in the main compounds or in the chemical markers but also in the low-concentration compounds, fingerprint analysis might be a simple way for identification and quality control of herbal products containing a large number of low amounts of unknown compounds. Furthermore in several papers the informations obtained from the analysis of a plant have been processed by statistical elaborations. Medicinal plants here discussed are classified on the basis of the chemical markers used for their quality control
Phenolic compounds from Bursera simaruba Sarg. bark: Phytochemical investigation and quantitative analysis by tandem mass spectrometry
No full textIntegrated mass spectrometric and multivariate data analysis approaches for the discrimination of organic and conventional strawberry (Fragaria ananassa Duch.) crops
No full textIn the present study, metabolic changes in secondary metabolites of Fragaria ananassa Duch. fruits during ripening were evaluated, and in particular, the differences between fruits obtained from either organic or conventional, non-organic crops. Extracts from ripe and unripe fruits of Fragaria ananassa var. Candonga were analyzed by direct infusion mass spectrometry using an instrument equipped with an ion trap analyzer (DI-ESI-IT-MS) and by liquid chromatography coupled to high resolution mass spectrometry using a mass spectrometer equipped with an ESI source and an Orbitrap analyzer (LC-ESI-FT-MS). Chromatograms were evaluated using the free software package MZmine and the resulting metabolomics data were processed. Data sets were analyzed by Principal Component Analysis (PCA) and Partial Least Squares Discriminant Analysis (PLS-DA). All the differences between the fruits collected from organic crops and conventional crops were underlined. In addition, LC-ESI-FT-MS analyses were useful to achieve a comprehensive characterization of metabolic profiles of strawberries (flavonoids, anthocyanins and catechins were identified in the extracts). Flavonoids and anthocyanins were present with similar metabolic profile in F. ananassa obtained from organic and conventional, non-organic crops with differences from ripe and unripe fruits. Phenolic compounds thus are not responsive for the classification observed by untargeted metabolomic approach. © 2015 Elsevier Ltd
Integrated mass spectrometry approach to profile proanthocyanidins occurring in food supplements: Analysis of Potentilla erecta L. rhizomes
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