1,721,017 research outputs found
The Link Among Neurological Diseases: Extracellular Vesicles as a Possible Brain Injury Footprint
Full text linkExtracellular vesicles (EVs), referred as membranous vesicles released into body fluids from all cell types, represent a novel model to explain some aspects of the inter-cellular cross talk. It has been demonstrated that the EVs modify the phenotype of target cells, acting through a large spectrum of mechanisms. In the central nervous system, the EVs are responsible of the wide range of physiological processes required for normal brain function and neuronal support, such as immune signaling, cellular proliferation, differentiation, and senescence. Growing evidences link the EV functions to the pathogenic machinery of the neurological diseases, contributing to the disease progression and spreading. Extracellular vesicles are involved in the brain injury by multimodal ways; they propagate inflammation across the blood brain barrier (BBB), mediate neuroprotection and modulate regenerative processes. For these reasons, extracellular vesicles represent a promising biomarker in neurological disorders as well as an interesting starting point for the development of novel therapeutic strategies. Herein, we review the role of the EVs in the pathogenesis of neurological disease, discussing their potential clinical applications
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Inositol lipid-mediated intranuclear signalling: a comparative analysis of in vivo labelling in interferon alpha-sensitive and -resistant Daudi lymphoma cells.
No full textDispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
The application of flow cytometry to the study of nuclear matrix. A multiparametric analysis.
No full textThe nuclear matrix, which in situ corresponds essentially to the interchromatin ribonucleoprotein particles, once isolated appears constituted by a peripheral lamina connected to the nucleolar remnant by a fibrous network. The features of the matrix components depend on the procedure employed during its purification, a multistep method commonly involving nuclease digestion, low and high salt extractions and treatment with a non ionic detergent. Here is reported the application of flow cytometry to the analysis of matrix purification. The experimental data indicate that, despite deep changes of the nuclear content due to the selective extraction of almost all nucleohistone and membrane components, the scatter pattern of the matrix, which is drastically lowered as the nucleic acid content decreases, closely resembles that of the starting nuclei. This behaviour permits one to follow the different matrix populations deriving from diploid and polyploid parenchymal liver nuclei throughout the isolation procedure and confirms the feasibility of flow cytometry for both analysis and sorting of nuclei and nuclear matrices. In addition, an ultrastructural analysis on thin sections and a morphometric study by means of semiautomated image analysis of phase contrast micrographs have been performed. The image analysis showed a decrease in particle dimension to about 50% of the nuclear value, which could partially explain the reduction of forward light scatter
Response of isolated nuclei to phospholipid vesicles: Effect of phosphatidylserine on alpha and beta DNA polymerase activity
No full textTo further our understanding of nuclei-phospolipid interaction,
we have investigated the possible involvement of PS in DNA syn-
thesis by checking the modifications of endogenous DNA alpha and
beta polymerase activities in normal rat liver nuclei
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