1,721,111 research outputs found
Metodi e strategie per le politiche di sviluppo locale nel settore dei servizi in sicilia: un sistema informativo statistico territoriale per le aree funzionali
No full textAMINO ACID, SUGAR, POLIPHENOL, VITAMIN C AND VITAMIN E CONTENTS IN FRESHLY HARVESTED FRUITS OF PLUM LANDRACES OF CAMPANIA REGION (ITALY)
No full textAMINO ACID, SUGAR, POLIPHENOL, VITAMIN C AND VITAMIN E
CONTENTS IN FRESHLY HARVESTED FRUITS OF PLUM LANDRACES
OF CAMPANIA REGION (ITALY)
IANNUZZI F., MIRTO A., WOODROW P., CARILLO P., FUGGI A.
Department of Environmental Biological and Pharmaceutical Sciences and Technologies, II
University of Naples, Via A. Vivaldi 43, 81100 Caserta (Italy)
plum biodiversity, sugars, poliphenols, vitamins, free amino acids
The characterization of traditional cultivars (landraces), selected by farmers and particularly
adapted to the environment in which they evolved is fundamental in the conservation and
enhancement of the Agro-biodiversity. Morphological, phenological and genetic methods, actually
are integrated by proteomics and metabolomics ones. Such methods evaluate phenotypic traits
modulated by the environmental conditions (abiotic and biotic stress). In food products they allow
the quality assessment at harvest, during postharvest storage and/or treatments. Metabolic profiling
can proved fingerprints of typical products useful to define, protect and track them along their
lifestory.
In this work sugars, organic acids, polyphenols, anthocyanins, Vitamin C (ascorbic acid and
dehidroascorbic acid) and vitamin E (α-tocopherol and γ-tocopherol), free amino acids, were
determined in fruits from plum landraces of Campania (Italy). Fruits from sixteen plum landraces
cultivated at "CRAA-Azienda Agricola Sperimentale Improsta" of Campania Region were
analysed. The edible part of freshly harvested fruits were cutted in small pieces, frozen in liquid
nitrogenand saved at -80°C. Three samples of cutted materials were powedered in liquid nitrogen
and used to prepare extracts for the analyses. The results were expressed on a fresh weight basis as
means and standard error.
The plum fruits showed a huge variability in the distribution of soluble sugars: they were
about 4% of fresh weight (FW) In the landrace "Sila", while in "Del Carmine" and "Pezza Rossa" it
was less than 1% of FW. Sorbitol content, on average, was about 18% of the soluble sugars. The
polyphenol contents were at least 100% higher than the USDA one. Higher levels of poliphenols
occurred in "Santa Paola", "Coglie e Astad Bianca” and “Santangiolese”, while the lower one was
found in "San Rafele" and "Pappacona". Among the analysed landraces the anthocyanins occurred
mainly in the skin, and, therefore, the amounts were, generally, lower than 1% of total polyphenols.
The Vitamin E (α, γ tocopherols) was similar to USDA average data in most of the landraces, while
they were more than 100% higher in "Santa Paola" and "Santangiolese". Only in some landraces γ
tocopherol content was higher than the α one. However Vitamin E was highly correlated to
polyphenol content. Among the free amino acids asparagine occurred at the highest concentration
followed by GABA, aspartate and glutamate. Proline was at the highest level in “Sila” and “Del
Carmine”. Organic acids and Vitamine C were also analysed.
PCA analysis of all data evidenced a main cluster with 12 landraces of the 16 analysed ones.
Such data can integrate the genetic and agronomic ones for a better characterization of plum
landraces.
The work was funded by "Regione Campania, PSR 2007/2013, Measure 214, Action f2,
project Agrigenet"
CDNA-NBS PROFILING OF HORTENSIA RGA-LIKE GENES RESPONSIVE TO LEAF SPOT FUNGAL INFECTION.
No full textCDNA-NBS PROFILING OF HORTENSIA RGA-LIKE GENES RESPONSIVE TO LEAF SPOT FUNGAL INFECTION.
WOODROW P., KAFANTARIS I., IANNUZZI F., MIRTO A., FUGGI A. AND CARILLO P.
Dipartimento di Scienze e Tecnologie Ambientali Biologiche e Farmaceutiche, Seconda Università degli Studi di Napoli, via Vivaldi 43, 81100 Caserta, Italy.
Keywords: biotic stress, resistance genes, nbs profiling, leaf spot, hortensia.
Hydrangeas are susceptible to leaf spot fungus Cercospora hydrangeae. Fungal spores germinate quickly and spread over the plant leaves under warm and rainy weather forming small purple or brown spots. Although Hydrangea plants are not killed by leaf spot, it reduces the flowering and plant vigor decreasing the market value of the plants. The aim of our study was to isolate and characterize Hortensia resistance (R) gene transcripts under C. hydrangeae fungus infection and investigate their expression profile and evolutionary relationships. The majority of disease R-genes in plants encode nucleotide-binding site leucine-rich repeat (NBS-LRR) proteins. A cDNA-NBS strategy was carried out using as template cDNAs isolated from control and infected plant leaves. The cDNA-NBS profiling gave an excellent bands reproducibility. Twenty new transcripts corresponding to NBS-LRR proteins were identified only in infected plants. Their phylogenetic analysis revealed that most of them evolved through concerted evolution probably due to a selective pressure from a certain pathogen and/or genetic evolutionary events such as gene conversion, crossing-over, duplication and diversifying selection
ASCORBIC ACID, ASCORBATE PEROXIDASE, AND H2O2 IN FRESHLY HARVESTED FRUITS OF SWEET CHERRY LANDRACES OF CAMPANIA (ITALY)
No full textASCORBIC ACID, ASCORBATE PEROXIDASE, AND H2O2 IN FRESHLY
HARVESTED FRUITS OF SWEET CHERRY LANDRACES OF CAMPANIA
(ITALY)
MIRTO A., IANNUZZI F., VACCA F., WOODROW P., CARILLO P., FUGGI A.
Department of Environmental Biological and Pharmaceutical Sciences and Technologies, Second
University of Naples, Via A.Vivaldi 43, 81100 Caserta (Italy)
sweet cherry,vitamin C, ascorbate peroxidase, hydrogen peroxide,redox potential
Ascorbate (AsA) has a key role in many metabolic processes, regenerating other metabolites
like tocopherols from oxidative damages and protect many enzymes from irreversible inactivation
by oxidation mainly caused by the oxidative stress. It is among the main component of the redox
homeostasis buffering mechanism (ascorbate - glutathione cycle and is involved in many redox
signalling processes. Ascorbate peroxidase (APX) as enzyme of the ascorbate-glutathione cycle
uses ascorbate as electron donor to scavenge hydrogen peroxide (H2O2) produced in normal and
stress enhanced aerobic metabolism. (Ascorbate (vitamin C) is essential for human health and its
main dietary source are plant-derived foods. In addition high level of ascorbate in fruits and
vegetables improve their post-harvesting storage properties.
In the aim of conservation and valorization of agro-biodiversity of sweet cherry germoplasm
of Campania (Italy) a study has been conducted to measure AsA, dehydroascorbate (DHA) and
hydrogen peroxide (H2O2) contents, as well as APX activities in the harvested fruits. The redox
state of the fruits was also evaluated.
Freshly harvested fruits from 23 landraces cultivated at the Regione Campania – Improsta
farm were used for the analyses.
The sweet cherry fruit extracts of the landraces varied significantly in contents of AsA and
DHA as well as in the DHA/AsA ratio. The redox potential showed the lowest value in the extracts
from the “Patanara” and “Lauretana” ones that did not show particularly high contents of AsA. The
highest potential was found in those from “Melella” and “Tamburella” that had relatively low AsA
content. Extracts from “Melella” were among those with the highest APX activity, while those
from “Tamburella” were from those with the lowest APX activity, as were also the extract from
Lauretana. The H2O2 contents also were not simply related to AsA content, APX activity and redox
potential, in agreement with the view that ascorbate concentration is dependent on biosynthesis,
catabolism and recycling. Anyway as considering that the oxidative stress in postharvest increase it
can be suggested that fruits with the lowest redox potential closer to the physiological one, at the
harvest,contains the overall most efficient antioxidant system and may be more suitable for postharvest
storage.
The work was funded by "Regione Campania, PSR 2007/2013, Measure 214, Action f2,
project “Agrigenet"
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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