362 research outputs found
Analysis of mitochondrial DNA of an endangered beech species, Fagus hayatae Palibin ex Hayata
No full textBasic fibroblast growth factor induces angiogenic properties of fibrocytes to stimulate vascular formation during wound healing
No full text主査 : 高橋啓 / タイトル : Basic fibroblast growth factor induces angiogenic properties of fibrocytes to stimulate vascular formation during wound healing / 著者 : Miho Nakamichi, Yuri Akishima-Fukasawa, Chie Fujisawa, Tetsuo Mikami, Kiyoshi Onishi, Yoshikiyo Akasaka / 掲載誌 : The American Journal of Pathology / 巻号・発行年等 : 186(12):3203-3216, 2016 / 本文ファイル: 出版者
Basic fibroblast growth factor induces angiogenic properties of fibrocytes to stimulate vascular formation during wound healing
No full text主査 : 高橋啓 / タイトル : Basic fibroblast growth factor induces angiogenic properties of fibrocytes to stimulate vascular formation during wound healing /著者 : Miho Nakamichi, Yuri Akishima-Fukasawa, Chie Fujisawa, Tetsuo Mikami, Kiyoshi Onishi, Yoshikiyo Akasaka /掲載誌 : The American Journal of Pathology /巻号・発行年等 : 186(12):3203-3216, 2016
Effects of CX3CL1 inhibition on murine bleomycin-induced interstitial pneumonia
No full text主査 : 岸一馬 / タイトル : Effects of CX3CL1 inhibition on murine bleomycin-induced interstitial pneumonia /著者 : Soichi Yamada, Shion Miyoshi, Junko Nishio, Satoshi Mizutani, Zento Yamada, Natsuko Kusunoki, Hiroshi Sato, Yoshikazu Kuboi, Kana Hoshino-Negishi, Naoto Ishii, Toshio Imai, Tetsuo Mikami, Hiroyasu Nakano, Shinichi Kawai, Toshihiro Nanki /掲載誌 : European Journal of Inflammation /巻号・発行年等 : 2.15277777777778 /本文ファイル: 出版者
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No full textThe Owen mitochondrial genome in sugar beet (Beta vulgaris L.): possible mechanisms of extensive rearrangements and the origin of the mitotype-unique region
The Femme Fatale and the Exotic Queer within Shinya Tuskamoto\u27s Tetsuo: Gender as Narrative Tool within an Allegory for Post WWII Japan\u27s Industrialized Identity Crisis
No full textWithin Shinya Tsukamoto’s seminal independent horror masterpiece Tetsuo, the viewer’s perceptions of reality and the present are distorted within a temporally disjointed blend of horrific fantasy and banal existence; this instability reflects the vocal and subconscious critiques of historical ontological truths exhibited within the emergent transnational genres of Japanese cyberpunk and American Avant-pop ideologies of the late 1980’s. Author Takayuki Tatsumi uses Shinya Tsukamoto\u27s Tetsuo to illustrate the emergence of the Japanoid, a technologically driven fusion of American and Japanese post-war identity best understood as a manifestation of Donna Haraway\u27s socio-political cyborg. Tatsumi strongly advises avoiding interpretation through a queer lens, proposing that the use of “cyborg” and scrap iron serve as an analogy for the stratification and integration of disenfranchised post WWII Okinawan “scrap apaches.” However, Tetsuo’s prominent homoerotic elements cannot be ignored. Arguably, The film presents as blatantly non-heteronormative; to ignore queerness and instead focus solely on Tatsumi\u27s definition of identity ignores the meaning of masculinity in a patriarchal culture, rendering an incomplete (post)colonial reading. A queer reading clarifies Tsukamoto\u27s take on the contemporary disenfranchisement of the so-called Japanoid identity that Tatsumi embraces. Within Tetsuo, representation of woman as femme fatale and an overt queering of masculinity problematize the traditional heteronormative Japanese identity
Inexpensive Fracture Toughness Testing of Welded Steel
No full textIn a prior project, TNO has presented a low-cost way of finding fracture toughness of base materials for cleavage fracture. That method features a small-scale CTOD specimen, combined with simplified sensors, less fatigue pre-cracking, faster testing, and no need for a temperature chamber. This method has been extended to welds by considering the effect of pop-ins. This paper summarizes the prior method and the justifications for it before extending it to welded structures by introducing adjustments for pop-ins for small-scale specimens.Accepted Author ManuscriptShip Hydromechanics and StructuresBUS/TNO STAF
Large 3' UTR of sugar beet rps3 is truncated in cytoplasmic male-sterile mitochondria
No full textGenomic alteration near or within mitochondrial gene is often associated with cytoplasmic male sterility (CMS). Its influence on the expression of the mitochondrial gene was proposed as one of the possible causes of CMS. In sugar-beet mitochondrial rps3, whose downstream 1056-bp region contains Norf246, an apparently non-functional open reading frame (ORF), was deleted in CMS mitochondria. In our previous study, normal rps3 (3.8 kb), CMS rps3 (2.7 kb), and Norf246 (3.8 kb and 0.9 kb) were shown to be transcribed. The present study was conducted to determine whether the deletion affected gene expression. Reverse transcription (RT)-PCR analysis revealed the co-transcription of rps3 and Norf246. By circularized RNA (CR) RT-PCR analysis, the 5' and 3' termini of the 3.8-kb and the 0.9-kb transcripts were determined. The results suggested that the 3.8-kb transcripts were the rps3 mRNA bearing an ∼464-base 5' untranslated region (UTR) and ∼1508-base 3' UTR, whereas no functional ORF was observed in the 0.9-kb transcripts. CR-RT-PCR revealed that the 3' UTR of the 2.7-kb transcripts was reduced to ∼460 bases. However, no difference in the accumulation of RPS3 polypeptide and RNA editing was detected by protein gel blot analysis and cDNA sequencing. Although the deleted region encoded the truncated-atp9 that was edited, no influence on the pattern and frequency of RNA editing of genuine atp9 was evident. The results eliminated rps3 as a candidate for the CMS gene, making preSatp6, a unique ORF fused with CMS atp6, the sole CMS-associated region in sugar beet
Design Contours for Complex Marine Systems
No full textThis paper examines the performance of 6 stiffened ship panel designs in different operational profiles. The main question of interest is: which sea states will lead to the worst panel performances in terms of reliability? As stiffened panel collapse is governed by combined lateral and in-plane loading effects (non-linear functions of the wave environment) this is not a simple problem and does not easily fit into the confines of traditional analyses. Interesting sea states for stiffened panel collapse are identified by a low-order design contour method which uses order statistics and extreme value theory. The resulting multimodal design contours pinpoint areas of interest and the panel performances are confirmed using a higher-order reliability analysis: the non-linear Design Loads Generator process. Such results have impact for creating and interpreting environmental and design contours, as well as assumptions about which operational profiles will lead to the worst system responses.Accepted Author ManuscriptShip Hydromechanics and StructuresShip Design, Production and Operation
DNA methylation is not necessary for the inactivation of the Tam3 transposon at non-permissive temperature in Antirrhinum
No full textIt has been proposed that DNA methylation plays an important role in the inactivation of transposons. This view stems from a comparison of the degree of methylation of transposons in the active and inactive state. However, direct evidence for the degree of methylation required for the suppression of transposition has not been reported. Transposon Tam3 in Antirrhinum majus undergoes somatic reversal of its transposition activity, which is tightly controlled by temperature: low temperature around 15℃ permits transposition, high temperatures around 25℃ strongly inhibits it. Our previous study had shown that the methylation state of the Tam3 end regions is negatively correlated with the Tam3 transposition frequency. The results of the present study reveal that the inactive state of Tam3 copies at high temperature is unlikely to be directly coupled to the methylation state. Treatment with methylation inhibitors (5-azacytidine or 5-azacytidine+ethionine) does not affect Tam3 excision frequency in calli derived from Antirrhinum hypocotyls. The results suggest that methylation is not essential for the suppression of Tam3 transposition at high temperature, but rather that some other mechanism(s) involved in the control of Tam3 transposition may be obscured by methylation
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