1,720,966 research outputs found
F-type lectin from serum of Trematomus bernacchii (Boulenger, 1902): purification, characterization and bacterial agglutinating activity
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Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
The multiple potentialities of anthozoans: analyses and comparisons between animal models
Full text linkAnthozoans are the richest class of species of the phylum Cnidaria. They are a candidate group for studying the evolution of mutualisms and immunity
and despite their morphological simplicity exhibit a repertoire of immunological components with large genomes and gene families similar to those of the Bilateria.
Like other invertebrates, anthozoans immunity is based on self/non-self recognition mechanisms and allorecognition responses, therefore, maintaining their integrity and responding actively to selection pressures.
Highlight and investigate the link between innate immunity, homeostasis maintenance, inflammation, tissue remodelling and regeneration in
Anthozoa could be useful to elucidate the adaptive capability features to different stress factors.
We have carried out studies demonstrating that all these processes are highly conserved among the anthozoans species. We have compared the inflammatory responses and the morpho-functional aspects related to regeneration in different species of Mediterranean anthozoans using histological, cellular and molecular technical approaches on organisms, maintained in aquaria under environmental and pathogenic stressful conditions.
This approach appears to be a useful tool from baseline studies in immunology and anthozoans result valid models able to respond to environmental stress conditions. Important results have been obtained with potential biotechnological transferability in pharmacology
Mucosal immunity response of European eel (Anguilla anguilla L.) after bacterial infections
No full textBasic approach of inflammation, injury and regeneration in Anemonia viridis
No full textThe potential for tissue regeneration is a powerful adaptive strategy essential to the survival of individuals. It allows to face wounds or loss of body parts induced by predation, anthropic actions or environmental factors. In light of the high probability of increasing levels of disturbances caused by injuries and the increasing possibility of invasion of microbes and foreign agents in the tissues of anthozoans, it is crucial to determine how the species respond to wounds and physical damage and understand the capacity of recovering and tissues regeneration. From this point of view, the regeneration capacity of Anthozoa it could be considered an additional arm of innate immune defence and viceversa. Therefore, from our work team, the inflammatory response in Mediterranean anthozoan Anemonia viridis (Forsskal, 1775) has been studied following the injection of substances of various type and size. We observed strong and specific reaction, especially after the bacterial injection of Escherichia coli and Vibrio alginolyticus. Then, we focused on the regenerative aspects of this species of anthozoan carrying out an experimental plan based on different numbers of tentacle cuts and the evaluation of the regenerative potential after 7, 14 and 21 days. Morphological observations and histological analysis on the tentacle regrowth, as well as measures of expression of proliferating cell nuclear antigen (PCNA) were carried out. Protease, phosphatase and esterase activities were measured as survival markers. In perspective, we want to study at histological and molecular level how homeostatic tissues start the regeneration program while triggering immune response and their mediators of inflammation. In this context, we will focus on those bioactive
molecules that, with their vast abundance, can be potentially used for biotechnological application
Actinins as novel broad-spectrum AMP isolated from the tentacle of Anthozoan Actinia equina (Linnaeus, 1758)
Full text linkCapturing activities and defense mechanisms of Cnidarian are strongly associated with toxins and
peptide with antimicrobial properties. AMP are an important component of many organisms’ innate
immune system with a good inhibitory or killing effect against invaders pathogens.
We investigated the AMP activity of acid extracts obtained from tentacle and body of Actinia
equina (Cnidaria, Anthozoa) against Gram positive (Micrococcus lysodeikticus) and Gram negative
(Escherichia coli, Vibrio alginolyticus) bacteria. The peptide fractions showed interesting minimum
inhibitory concentrations (MIC) values (concentrations up to 0.125 μg/ml) against tested
pathogens. Tentacle acid extracts exhibiting a good antimicrobial activity, were further investigated,
characterized and the peptides purified by reverse phase chromatography on solid phase Sep-Pak C8
column followed by several HPLC runs on C18 column. A broad-spectrum antibacterial peptides
activity was detected in 40 % acetonitrile fractions.
The Peptide 6.2 has a molecular weight of 2612.91 Da and is composed of 27 amino acids
(Actinin A); while peptide 7.3 has a molecular weight of 4323.07 Da and is composed of 35 amino acids
(Actinin B).
The two peptides were completely sequenced and their aa sequence revealed similarity with the
already described AMPs identified in amphibians and fish, with anti-Gram+ & Gram-, antifungal,
candidacidal, anti- Methicillin-resistant Staphylococcus aureus (MRSA) activity Actinins A and B were chemically synthesized and tested in vitro against the above-mentioned bacterial
pathogens. The analysis identified the peptide Actinin B which showed an interesting antibacterial
and can be considered good candidates for new therapeutic applications
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