1,721,087 research outputs found
Calamintha incana (Sm.) Helder: A New Phytoextract with In Vitro Antioxidant and Antidiabetic Action
This study aimed to assess the antioxidant and antidiabetic properties of ethanolic phytoextracts of Calamintha incana (Sm.) Helder leaves. Initially, the chemical characterization of the phytocomplex was performed using high-performance liquid chromatography (HPLC)/mass spectrometry (MS). The cytotoxicity of the ethanolic extract was assessed using an MTT assay in HepG2 cells. Subsequently, antioxidant activity was evaluated using a DPPH test. Finally, enzymatic tests with alpha-amylase, alpha-glucosidase, pancreatic lipase, and dipeptidyl peptidase IV (DPP-IV) were performed to evaluate their effects on glucose metabolism. The chemical composition of the extract is p-linolenic acid (13.2%), myristic acid (12.1%), and p-cymene (10.5%). The extract demonstrated low toxicity, with none of the tested concentrations inducing 50% cell death. Furthermore, the ethanolic extract revealed potent antioxidant activity using DPPH (IC50 was 35.9 +/- 0.7 mu g/mL) and reducing power capacity (IC50 was 90.3 +/- 0.8 mu g/mL). Regarding the antidiabetic activity, the extract caused a significant inhibition of alpha-amylase, alpha-glucosidase (IC50 46.3 +/- 0.2, 56.8 +/- 0.1 mu g/mL, respectively), weak inhibition of pancreatic lipase and no notable inhibition of dipeptidyl peptidase IV. In conclusion, C. incana has antioxidant and antidiabetic properties and appears to exert insulin-independent hypoglycemic action
Plant sentience: feeling or biological automatism?
Sentience refers to the ability of an organism to have subjective experiences such as sensations, emotions and awareness. Whereas some animals, including humans, are widely recognized as sentient, the question of whether plants are sentient is still debated among scientists, philosophers, and ethicists. Over the past 20 years, many scientists such as Trewavas, Baluška, Mancuso, Gagliano, and Calvo have reported interesting discussions about memory, behavior, communication, and intelligence in plants. However, the reported conclusions have not convinced the entire scientific community. In this commentary, I would like to focus on two critical aspects related to sentience: cognition and emotio
Effective Treatments for the Successful Establishment of Milkweed (Calotropis procera L.) under Water Deficit
The application of superabsorbents to soils and seed coatings is a pre-sowing seed treatment method that is commonly used to improve early vigor and establish stability and uniformity under water deficit conditions. To evaluate the interaction of seed coating and superabsorbent on Calotropisprocera L. (milkweed) under water deficit conditions, a greenhouse experiment was conducted. The experiment was conducted with four coating material levels (non-coated seeds and seeds coated with peat moss, vermicompost, and canola residue), four growth medium levels (soil, sand + soil, soil + 2 g superabsorbent, and soil + 4 g superabsorbent), and three field capacity regimes (25, 50%, and 100%) in a completely randomized design factorial arrangement with four replications. Reducing the field capacity from 100 to 25% led to decreased growth (shoot and root dry weights and leaf area) and chlorophyll content. The activities of SOD, CAT, APX antioxidant enzymes, and proline increased under drought stress. The use of superabsorbent polymers in growth media enhanced growth indices and chlorophyll content and decreased the activity of antioxidant enzymes and proline under water deficit conditions. The highest chlorophyll and growth indices were observed when 4 g of superabsorbent was added to the growth medium under drought stress. The application of 4 g of superabsorbent to the growth medium reduced the activity of antioxidant enzymes and proline. The use of seed coatings improved the growth indices, antioxidant enzyme activity, and chlorophyll content under drought stress. The most adaptive morphological and physiological responses to water stress were observed in the vermicompost-coated seeds. The vermicompost coating containing a superabsorbent polymer (4 g/kg soil) proved to be the best for establishing milkweed under mild (50% FC) and severe water deficits (25% FC)
Considerazioni cliniche e diagnostiche su un caso di toxocariasi a prevalente interessamento viscerale
Mycorrhizal Symbiosis Can Change the Composition of Secondary Metabolites in Fruits of Solanum nigrum L
Solanum nigrum is a common weed in arable land, while being used in traditional medicine around the world due to its remarkable levels of valuable secondary metabolites. Agronomic and biological techniques can alter the production of a specific metabolite by influencing plant growth and metabolism. The effects of colonization with three arbuscular mycorrhizal fungi (AMF), including Funneliformis mosseae, Rhizoglomus intraradices, and Rhizoglomus fasciculatum, on the chemical composition of S. nigrum fruits were evaluated by gas chromatography-mass spectrometry (GC-MS) analysis. More than 100 different chemical constituents were evaluated by GC-MS. Our study revealed that the levels of phenols (quinic acid), benzenes (hydroquinone), sulfur-containing compounds, lactone and carboxylic acids were improved by R. intraradices. In contrast, hydroxymethylfurfural increased by 68 % in R. fasciculatum inoculated with uninoculated S. nigrum plants, and this species was also the most efficient in inducing sugar compounds (D-galactose, lactose, and melezitose). Our results suggest that AMF colonization is an effective biological strategy that can alter the chemical composition and improve the medicinal properties of S. nigrum
Going Beyond Counting First Authors in Author Co-citation Analysis
The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Bioactive properties and metabolite profiling of Satureja species: antioxidant potential, anti-amylase activity, and nutritional composition
The Satureja genus, an aromatic herb, is traditionally used in culinary practices, fragrance production, and food manufacturing owing to its diverse bioactive properties. This study assessed the antioxidant capacity, anti-amylase activity, proximate composition, techno-functional properties, essential oil profile, fatty acid and amino acid composition, and overall nutritional value of selected Satureja species. The major macronutrients found in Satureja species are carbohydrates, proteins, and fats, while ash, polyphenols, chlorophylls, and carotenoids are present in lower concentrations. The powdered extracts of Satureja display strong antioxidant and α-amylase inhibitory activities, comparable to those of standard controls. These functional properties are likely related to the plant’s diverse biochemical composition, particularly its non-starch polysaccharides, proteins, lipids, polyphenols, flavonoids, chlorophylls, and carotenoids, which together enhance its nutritional value and bioactive potential. Essential oils were dominated by carvacrol, followed by γ-terpinene, thymol, p-cymene, and α-terpinene. The principal fatty acids included linolenic (ω-3), palmitic, linoleic (ω-6), oleic (ω-9), stearic, and palmitoleic (ω-7) acids, yielding favorable nutritional indices, such as high health-promoting and hypocholesterolemic scores and low atherogenicity and thrombogenicity values. Amino acid profiling revealed aspartic and glutamic acids as predominant, along with arginine, alanine, proline, leucine, serine, lysine, and glycine. Functional categorization indicated glucogenic amino acids as the most abundant, followed by hydrophobic, bitter, sweet, ketogenic, and branched-chain groups. Collectively, these findings highlight the Satureja genus as a valuable functional and techno-functional ingredient with potential applications in managing oxidative stress and preserving natural foods
Salicylic Acid Stimulates Defense Systems in Allium hirtifolium Grown under Water Deficit Stress
Nowadays, the use of the growth regulator salicylic acid for improving a plant’s resistance to environmental stresses such as drought is increasing. The present study investigated the effect of salicylic acid on the physiological traits, antioxidant enzymes, yield, and quality of Allium hirtifolium (shallots) under drought conditions for three years (2016–2017, 2017–2018, and 2018–2019). The experiment was conducted as a split-plot based on a randomized complete block design with four repeats. Irrigation as the main factor in four levels of 100% (full irrigation), 75% and 50% of the plant water requirements with non-irrigation (dryland), and salicylic acid as the sub-factor in four levels of 0, 0.75, and 1 mmol, were the studied factors in this research. The combined analysis of three-year data showed that drought reduced leaf relative water content (RWC), membrane stability index (MSI), chlorophyll content, onion yield, and increased activity of antioxidant enzymes, proline content, tang, and allicin of shallots. Shallot spraying with salicylic acid improved leaf RWC, MSI, chlorophyll content, and onion yield. The highest yield of onion (1427 gr m−2 ) belonged to full irrigation and foliar application of 1 mmol salicylic acid. The lowest yield (419.8 gr m−2 ) belonged to plats with non-irrigation and non-application of salicylic acid. By improving the effective physiological traits in resistance to water deficit, salicylic acid adjusted the effects of water deficit on the yield of shallots. Foliar application of 1 mmol salicylic acid in dryland and irrigation of 50% of the plant water requirement increased onion yield by 15.12% and 29.39%, respectively, compared to the control treatment without salicylic acid
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