1,721,002 research outputs found

    Erratum: Identification of the goat CSN1S1F allele by means of PCR-RFLP method (Animal Genetics (2000) 31 (342))

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    Erratum Ramunno L., Cosenza G., Pappalardo M., Pastore N., Gallo D., Di Gregorio P. & Masina P. (2000) Identi®cation of the goat CSN1S1F allele by means of PCR-RFLP method. Animal Genetics 31, 34

    A PCR polymorphism detected in the goat as2-casein gene

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    PCR primers, VQ5 and VQ14, were designed from regions. of goat as2-Cn cDNA sequence (GenBank accession no X65160)' corresponding to exons 17 and 18 of bovine as2-Cn gene (GenBank accession no. M943227).' The amplified DNA fragments revealed a polymorphism. Two alleles were observed: the A allele (showing a fragment of about 3300 bp) and the B allele (showing a fragment of about 3700 bp). Frequencies of the two alleles determined in 150 unrelated goats of undefined genetic type reared in Southern Italy were 0.6 (A) and 0.4 (B)
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