1,721,076 research outputs found
Scratch Wound Healing Assay
No full textCell migration is a crucial step for wound healing. Assays able to evaluate cell migration are very useful to evaluate in vitro wound healing. Scratch wound assay creates a gap in confluent monolayer of keratinocytes to mimic a wound. The protocol of scratch wound is based on few steps: cell culture preparation, scratch wound assay, data acquisition, and data analysis
Lo sprawl
No full textIl contributo presenta alcune riflessioni sul fenomeno dello sprawl, sottolineandone la diffusione negli Stati Uniti e poi in Europa. Evidenzia inoltre l'impatto ambientale e sociale del fenomeno, in particolare sul consumo di suolo e sullo stile di vita di chi va a vivere nello sprawl stesso. Illustra infine alcuni recenti recenti di insediamenti sostenibili, orientati cioè a un basso livello di emissioni e a un basso consumo di suolo
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Ascorbate and cancer therapy: Possible support from dietary habits
No full textAscorbate is an essential nutrient in the human diet, but is also widely used as a medicinal product, and has long been held as a remedy for various diseases. Several studies have suggested a possible chemotherapeutic use of ascorbate, but there are discrepant observations possibly derived from different ways of ascorbate administration.In this chapter, we will focus on the anticancer effect of ascorbate, with particular emphasis on production of reactive oxygen species. When pharmacological ascorbate is given via intravenous administration, it is possible to achieve millimolar plasma concentration. At these levels, and in the presence of catalytic metal ions, ascorbate can induce oxidative stress through the generation of hydrogen peroxide. Recent in vitro and in vivo observations have demonstrated that ascorbate oxidation occurs in the extracellular space. This oxidation generates a ROS flux through cell membrane producing oxidative stress inside the cells, damaging severely cancer cells rather than normal cells. We will also review present data that supports ascorbate potential use in cancer therapy. Ascorbic acid or Vitamin C is a water-soluble vitamin. Vitamin C can be produced by most animals and plants from D-glucose and D-galactose. Ascorbic acid production is not possible in the human body due to the lack due to the lack of L-gulonolactone oxidase enzyme, and therefore, it should be assumed with the diet. © 2017 Nova Science Publishers, Inc
AQP3 wound healing: is honey a new possible link?
No full textAQP3 plays an important role in hydration of mammalian skin epidermis, but also in the regulation of proliferation and differentiation of keratinocytes, being, therefore, important in wound healing. Since Biblical times, honey has been used in "folk medicine", and more recently, it has been successfully used on infections not responding to standard antiseptic and antibiotic therapy, in fact, it is well documented the honey production of H2O2. Etc..
Endothelial response boosted by platelet lysate: the involvement of calcium toolkit.
No full textWound repair is a dynamic process during which crucial signaling pathways are regulated by growth factors and cytokines released by several kinds of cells directly involved in the healing process. However, the limited applications and heterogeneous clinical results of single growth factors in wound healing encouraged the use of a mixture of bioactive molecules such as platelet derivatives for best results in wound repair. An interesting platelet derivative, obtained from blood samples, is platelet lysate (PL), which has shown potential clinical application. PL is obtained from freezing and thawing of platelet-enriched blood samples. Intracellular calcium (Ca2+) signals play a central role in the control of endothelial cell survival, proliferation, motility, and differentiation. We investigated the role of Ca2+ signaling in the PL-driven endothelial healing process. In our experiments, the functional significance of Ca2+ signaling machinery was highlighted performing the scratch wound assay in presence of different inhibitors or specific RNAi. We also pointed out that the PL-induced generation of intracellular ROS (reactive oxygen species) via NOX4 (NADPH oxidase 4) is necessary for the activation of TRPM2 and the resulting Ca2+ entry from the extracellular space. This is the first report of the mechanism of wound repair in an endothelial cell model boosted by the PL-induced regulation of [Ca2+]i
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