23,190 research outputs found
Functional Genomics for the Study of Fruit Ripening and Quality: Towards an Integrative Approach
No full textBuckypaper (BP) as absorbable adhesive for surgical application in abdominal wall issues
No full textBy: Sun, P.; Hu, S. B.; Cheng, X.; Li, M.; Guo, B.; Song, Z. F.; Zhang, Y.; Zheng, Q. C.; Hoyuela, C.; Vega, L.; Carvajal, F.; Blay, L.; Juvany, M.; Camps, J.; Veres, A.; Besora, P.; Trias, M.; Feliu, X.; Bellanova, G.; Viel, G.; et al. In: Hernia. April 2015 19(1):S157-S165; Springer Paris Language: English, Database: Springer Nature Journals
PROTPCOLLO DI RICERCA PUBBLICATO SU: ClinicalTrials.gov Identifier: NCT02137018 https://register.clinicaltrials.gov FINANZIAMENTO RICERCA DI ATENEO INTERDIPARTIMENTALE prot. C26A10HCNR, founding of 35000€ DIPARTIMENTO DI CHIMICA: Prof Lucio D'Ilario, Prof. Andrea Martinelli, DIPARTIMENTO DI CHIRURGIA "PARIDE STEFANINI": Dott. Massimo Chiaretti , prot letter 0003306 project n° 304P25, interfaculty Chemistry Dep, General Surgery Paride Stefanini Dep. http://www.uniroma1.it Location National Health Institute (ISS Istituto Superiore di Sanità), Viale Regina Elena 299 Rome, Roma, Italy, 00161 "BP as a New Device for Surgery and Solid Cancer and Hematopoietic System Tumors Treatment. Effects of BP Implantation" (DM159) ClinicalTrials.gov Protocol and Results Registration System (PRS) Receipt Release Date: 12/30/2014 The objective of this project is to obtain results that can direct the search for the ultimate realization of a prosthetic device for use in abdominal surgery. Will be conducted systematic experiments in 30 New Zealand female rabbits (R1-R30), weighting about 3000 g (Harlan Laboratories).Campanelli, Giampiero. Hernia Volume: 19 Issue S1 (2015) ISSN: 1265-4906 Online ISSN: 1248-9204
Buckypaper (BP) as absorbable adhesive for surgical application in abdominal wall issues
Chiaretti M(1), Martinelli A(2), Carru GA(3), Alunni Fegatelli D(4), Chiaretti AM(5), Consentino P(6), Chiaretti AI(1), Procacciante F(1)
(1) Department of General Surgery “Paride Stefanini”, Sapienza University, Viale del Policlinico 155, Rome, 00161 (Italy)
(2) Department of Chemistry, Sapienza University, P.le Aldo Moro 5, Rome, 00185 (Italy)
(3) Policlinico Umberto I Hospital, Sapienza University, Viale del Policlinico 155, 00161 - Rome (Italy)
(4) Public Health and Infective Illness, Sapienza University, P.le Aldo Moro 5, Rome, 00185 (Italy)
(5) Istitute of Genetic Risearch G. Salvatore, Scienze e Tecnologie Genetiche Biogem;
(6) Appialab Srl V. Latina, 286 00179 Rome (+39) 067825111www.appialab.it
E-mail: [email protected]
Introduction: One of the key issues relating to abdominal prosthetic surgery is the fixation of the abdominal mesh. Mesh displacement, improper or blind application of metal tacks and staples in laparoscopic surgery are the main causes of complications such as seroma[1], postoperative pain[2] and recurrence of hernia[3]. The well-established use of fibrin glue[4], or self-gripping commercial meshes, or suture meshes to fix prosthesis to damaged tissue may represent a risk associated to the transmission of unknown diseases related to the use of blood-derived materials[4] or pain caused by nerve entrapment or displacement. Moreover, it has been observed[1,5] that the use of fibrin glue for mesh fixation may be associated with incidence of postoperative seroma. An effective alternative to the conventional prosthesis fixation methods may be represented by devices able to adhere strongly and quickly[1,5] on wet[6] biological tissue. In view of a possible application of BP as adhesive absorbable tape in surgery, preliminary in vivo experiments were carried out on large-size pigs. Methods: We investigated the potential applicability of BP, a self-standing felt composed of multi-walled carbon nanotubes, as an adhesive tape on biological tissue compared with traditional methods of fixation of prosthetic meshes. Based on biological materials from experimentation on rabbits (in progress[7]) we carried out a comparative study on two sets of meshes: one of the sets was fixed with BP and the other set was fixed with traditional methods. We did this by carrying out bench surgery and performing mechanical peeling and shear adhesiveness tests on each set[5,8,9,10]. The in vivo experimentation was carried out on 30 Landrace female pigs weighing about 15 kg initially and 60 kg when they were sacrificed. The subjects were divided into two sets i.e., 15 for laparoscopic procedure and 15 for laparotomic procedure. Each group was further subdivided into an experimental set of 11 subjects for mesh fixation with BP and a control set of 4 subjects for traditional mesh fixation. Results: Experimental and control subsets did not show mortality or morbidity. Even after 90 days from the operation no significant neurovegetative or behavioral differences were observed. The BP fixing method shows stronger adhesiveness on wet tissues[6] than the mentioned traditional fixing methods. Checks on body weight monitoring curves show no significant differences between BP and control groups. Necroscopical and histological investigations show that BP elicits minimal adverse tissue response. Nanometric carbon nanotube aggregates were phagocytized by macrophages. Further, BP fragments were also observed in Bowman's urinary space. Finally, around the BP, a capsule of loose fibrous tissue is formed by collagen fibers deposited by fibroblasts which indicate a moderate cicatrisation reaction. Conclusion: Findings from our experiment are compatible with a stronger adhesiveness on wet biological tissue for the BP fixing method compared to traditional methods. Further studies are required to fully assess the nature of the BP debris, their toxicity potential and their metabolization.
References
1. B. P. Jacob, N. J. Hogle, E. Durak, T. Kim, D. L. Fowler, Surg. Endosc. 2007, 21, 629
2. J. R. Eriksen, J. I. Bech, D. Linnemann, J. Rosemberg, Hernia 2008, 12, 483
3. W. B. Gaertner, M. E. Bonsack, J. P. Delaney, Hernia 2010, 14, 375
4. S. Olmi, A. Addis, C. Domeneghini, A. Scaini, E. Croce, Hernia. 2007, 11, 211
5. S. Bellucci, M. Chiaretti, P. Onorato, F. Rossella, M. S. Grandi, P. Galinetto, I. Sacco, F. Micciulla, Nanomedicine 2010, 5, 209
6. A. Martinelli, GA. Carru, L. D'Ilario, F. Caprioli, M. Chiaretti, F. Crisante, I. Francolini, and A. Piozzi. ACS Appl. Mater. Interfaces, DOI:10.1021/am400543s Publication Date (Web): 18 Apr 2013, http://pubs.acs.org on April 19, 2013
7. M. Chiaretti, G. A. Carru, Danilo Alunni Fegatelli, A. M. Chiaretti, A. Martinelli, (in progress)
8. M. Chiaretti, G. Mazzanti, S. Bosco, S. Bellucci, A. Cucina, F. Le Foche, G. A. Carru, S. Mastrangelo, A. Di Sotto, R. Masciangelo, A. M. Chiaretti, C. Balasubramanian, G. De Bellis, F. Micciulla, N. Porta, G. Deriu, A. Tiberia, J. Phys.: Condens. Matter 2008,20, 474203
9. A. Di Sotto, M. Chiaretti, G. A. Carru, S. Bellucci, G. Mazzanti, Toxicol. Lett. 2009, 184, 192
10. S. Bellucci, M. Chiaretti, A. Cucina, G.A. Carru,A.I. Chiaretti, Nanomedicine 2009, 4, 53
Buckypaper (bp) as absorbable adhesive for surgical application in abdominal wall defects
No full textTOPICS: Biotecnologia ed innovazioni tecnologiche. Le proposte italiane nella ricerca.
BUCKYPAPER (BP) AS ABSORBABLE ADHESIVE FOR SURGICAL APPLICATION IN ABDOMINAL WALL DEFECTS
Massimo Chiaretti(1), Andrea Martinelli(2), Giovanna Angela Carru(3), Emanuela D’Amore(4), Alessandra Maria Chiaretti(5), Fabio Faiola(6), Paola Consentino(7), Eleonora Valente(8), Annalisa Italia Chiaretti(1)
(1)Department of General Surgery “Paride Stefanini”, Sapienza University of Rome, Viale del Policlinico 155, Rome, 00161 (Italy)
(2)Department of Chemistry, Sapienza University of Rome, P.le Aldo Moro 5, Rome, 00185 (Italy)
(3)Policlinico Umberto I Hospital, Sapienza University of Rome, Viale del Policlinico 155, 00161 - Rome (Italy)
(4)National Health Institute, Viale Regina Elena 299, 00161 - Rome (Italy)
(5) Istitute of Genetic Risearch G. Salvatore, “Consorzio Interuniversitario di Scienze e Tecnologie Genetiche Biogem”;
(6)Department of Molecular Medicine, Sapienza University of Rome, Viale Regina Elena 324, Rome, 00161 (Italy)
(7)Appialab Srl V. Latina, 286 00179 Rome (+39) 067825111www.appialab.it
(8)Policlinico Agostino Gemelli, Università Cattolica del Sacro Cuore, Roma
E-mail: [email protected]
Keywords: buckypaper, BP, MWCNTs, medical application, abdominal wall defects surgery, wet adhesion, absorbable adhesive device.
Introduction: One of the main problems related to the abdominal prosthetic surgery is the mesh fixation. Mesh displacement, improper and blind application of metal tacks and staples in laparoscopy surgery are the main causes of complications such as seroma, postoperative pain and recurrence[6-9]. The use of fibrin glue between the prosthesis and the damaged tissue, although it has become a well-established surgical procedure, represents a possible risk associated to the transmission of unknown diseases related to the use of human blood-derived materials [9]. Moreover, it was observed that an increased incidence of postoperative seroma is associated to the use of fibrin glue for mesh fixation[10]. Surfaces able to adhere promptly and strongly on wet biological tissue may represent an effective alternative to the conventional prosthesis fixation methods. This study realized on animal model proposes the application of the nature inspired micro- or nano-patterned adhesive surfaces, by exploiting the scaling effect, according to which the adhesion strength can be enhanced through the reduction of interface adhesive contact size[12]. We experimented evidences on the potential applicability on biological tissue of the BP as an adhesive tape, a self-standing felt composed of entangled multi-walled carbon nanotubes. In vitro bench surgery, mechanical peeling, and shear adhesion tests, and In vivo tests were tested with prosthetic meshes[2,3,4,10]. BP shows a stronger adhesion, but only on wet tissues[12] much better than both self-gripping commercial mesh and fibrin-glue non-gripping meshes and fabrics. Prompted by these results, we implanted BP in pigs, to assess BP effectiveness as adhesive absorbable prosthetic device and its biocompatibility. After 90 days from the operation we observed no pig behavioral alteration. BP samples preserved their position in the implantation site and mechanical adhesion was enhanced, both in laparoscopy and laparotomic procedure. Materials and Methods. Operated 30 Lantrace female 40-50 Kg pigs, 15 in laparoscopic procedure and 15 in laparotomic procedure. Operated and control subjects did not show mortality or morbidity, no significant neurovegetative or behavioral differences. Results and Discussion. Body weight monitoring graph do not showed any significant difference between BP and control group. The Hematoxilin and Eeosin stained implant section reported BP surface fragmentation that shows the formation of a capsule of loose fibrous tissue, consisting of fibroblasts and collagen fibers, indicative of a weak inflammation reaction. The necroscopy examination showed that the implanted BP favors a cicatrisation process, the mesh appearing wrapped in the inflammatory reaction. Conclusions: a strong BP adhesion on wet biological tissue was measured. In view of a possible application as adhesive absorbable tape in surgery, preliminary in vivo experiments were carried out on big pig model. Necroscopical and histological investigations enlighten that as 90 days after the implantation the BP elicits minimal adverse tissue response. Nanometric carbon nanotube aggregates were phagocytised by macrophages and observed in Bowman's urinary space. The assessment of the BP debris, possible toxicity or confinement or metabolism and accumulation or excretion mechanism needs further studies.
References
1. L. Liu, W. Ma, Z. Zhang,Small 2011, 7, 1504
2. M. Chiaretti, G. Mazzanti, S. Bosco, S. Bellucci, A. Cucina, F. Le Foche, G. A. Carru, S. Mastrangelo, A. Di Sotto, R. Masciangelo, A. M. Chiaretti, C. Balasubramanian, G. De Bellis, F. Micciulla, N. Porta, G. Deriu, A. Tiberia, J. Phys.: Condens. Matter 2008,20, 474203
3. A. Di Sotto, M. Chiaretti, G. A. Carru, S. Bellucci, G. Mazzanti, Toxicol. Lett. 2009, 184, 192
4. S. Bellucci, M. Chiaretti, A. Cucina, G.A. Carru,A.I. Chiaretti, Nanomedicine 2009, 4, 531
5. a) The mean equivalent radius was evaluated by wicking tests carried out in water, assuming a water contact angle of about 80°; b) G. Callegari, I. Tyomkin, K. G. Kornev, A.V. Neimark, Y-L. Hsieh, J. Colloid Interface Sci.2011, 353, 290
6. B. P. Jacob, N. J. Hogle, E. Durak, T. Kim, D. L. Fowler, Surg. Endosc. 2007, 21, 629
7. J. R. Eriksen, J. I. Bech, D. Linnemann, J. Rosemberg, Hernia 2008, 12, 483
8. W. B. Gaertner, M. E. Bonsack, J. P. Delaney, Hernia 2010, 14, 375
9. S. Olmi, A. Addis, C. Domeneghini, A. Scaini, E. Croce, Hernia. 2007, 11, 211
10. S. Bellucci, M. Chiaretti, P. Onorato,F. Rossella, M. S. Grandi, P. Galinetto, I. Sacco,F. Micciulla, Nanomedicine 2010, 5, 209
11. massimochiaretti.wordpress.com
12. A. Martinelli, GA. Carru, L.D'Ilario, F. Caprioli, M. Chiaretti, F. Crisante, I. Francolini, and A. Piozzi. ACS Appl. Mater. Interfaces, DOI:10.1021/am400543s • Publication Date (Web): 18 Apr 2013, http://pubs.acs.org on April 19, 201
Identifying Host Molecular Features Strongly Linked With Responses to Huanglongbing Disease in Citrus Leaves
No full textA bioinformatic analysis of previously published RNA-Seq studies on Huanglongbing (HLB) response and tolerance in leaf tissues was performed. The aim was to identify genes commonly modulated between studies and genes, pathways and gene set categories strongly associated with this devastating Citrus disease. Bioinformatic analysis of expression data of four datasets present in NCBI provided 46–68 million reads with an alignment percentage of 72.95–86.76%. Only 16 HLB-regulated genes were commonly identified between the three leaf datasets. Among them were key genes encoding proteins involved in cell wall modification such as CESA8, pectinesterase, expansin8, expansin beta 3.1, and a pectate lyase. Fourteen HLB-regulated genes were in common between all four datasets. Gene set enrichment analysis showed some different gene categories affected by HLB disease. Although sucrose and starch metabolism was highly linked with disease symptoms, different genes were significantly regulated depending on leaf growth and infection stages and experimental conditions. Histone-related transcription factors were highly affected by HLB in the analyzed RNA-Seq datasets. HLB tolerance was linked with induction of proteins involved in detoxification. Protein–protein interaction (PPI) network analysis confirmed a possible role for heat shock proteins in curbing disease progression
Protecting Animals 36: Author Witi Ihimaera
No full textIn this very special episode of Knowing Animals I am joined by beloved New Zealand author Witi Ihimaera. Witi has written many books featuring nonhuman animals. He offers us a non-colonial lens through which to think about the human/nonhuman relationship
Identifying early host response and countermeasures in citrus to biotic and abiotic stress.
No full textBeyond the Individual-Society Dualism. Georg Simmel's Lesson
No full textSimmel’s lesson highlights some possible weaknesses of the anthropological vision that supports the modern and, in some ways, also the post-modern socio-economic model: the individual-society dualism. It identifies some challenges in terms of thought and practice in order to overcome that dualism, but without slipping in monism – the biggest of all being the challenge of re-thinking the origin and its “unity”, through an alternative conceptualisation of the individual-society relationship
Freiheit
No full textThe theme of freedom is crucial in the field of social sciences. Social relationships, patterns and forms of organised life originate from and develop around the conceptualisation of freedom. And this is what Georg Simmel well understood when he pointed out that sociology can be thought as the science of relational freedom: the analysis of freedom is closely intertwined with the analysis of the individual-society relationship, in the dialectical perspective of reciprocity between individuals and social life, beyond deterministic, mechanistic and individualising stances. According to Simmel, it is clear that people are free only in relation to a social world that, in turn, establishes them as single individuals, and that their freedom has to do with others’ freedom and vice versa
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