1,721,020 research outputs found
H1TF2A: The large subunit of a heterodimeric glutamine rich CCAAT binding transcription factor involved in histone H1 cell cycle regulation
No full textH1TF2 is a CCAAT transcription factor that binds to the histone H1 subtype-specific consensus sequence, which has previously been shown to be necessary for temporal regulation of histone H1 transcription during the cell cycle (F. La Bella, P. Gallinari, J. McKinney, and N. Heintz, Genes Dev. 3:1982-1990, 1989). In this study, we report that H1TF2 is a heteromeric CCAAT-binding protein composed of two polypeptide doublets of 33 and 34 kDa and 43 and 44 kDa that are not antigenically related. The 33- and 34-kDa species were not detected in our previous studies (P. Gallinari, F. La Bella, and N. Heintz, Mol. Cell. Biol. 9:1566-1575, 1989) because of technical problems in detection of these heavily glycosylated subunits. The cloning of H1TF2A, the large subunit of this factor, reveals it to be a glutamine-rich protein with extremely limited similarity to previously cloned CCAAT-binding proteins. A monospecific antiserum produced against bacterially synthesized H1TF2A was used to establish that HeLa cell H1TF2A is phosphorylated in vivo and that, in contrast to the H2b transcription factor Oct1 (S. B. Roberts, N. Segil, and N. Heintz, Science 253:1022-1026, 1991; N. Segil, S. B. Roberts, and N. Heintz, Cold Spring Harbor Symp. Quant. Biol. 56:285-292, 1991), no gross change in H1TF2A phosphorylation is evident during the cell cycle. Further immunoprecipitation studies demonstrated that H1TF2 is heterodimeric in the absence of DNA in vivo and identified several H1TF2-interacting proteins that may play a role in H1TF2 function in vivo
H1TF2A - A NOVEL HUMAN TRANSCRIPTION FACTOR INVOLVED IN HISTONE H1 CELL-CYCLE REGULATION
No full textShort and highly efficient synthetic promoters for melanoma-specific gene expression.
Full text linkHere, we report the construction and functional analysis of synthetic promoters designed for gene therapy applications requiring strong and specific gene expression in melanoma cell lines. We have analysed the transcriptional activity of different combinations of two transcriptional regulatory modules, a melanocyte-specific element from the human tyrosinase promoter and a cell-cycle-specific element from the human alpha-fetoprotein promoter. Transient expression assays in different cell lines show that several of these composite synthetic promoters can drive a strong and selective expression of a reporter gene in melanoma cell, providing us with a new powerful tool for gene therapy of melanomas
H1TF2A: The large subunit of a heterodimeric glutamine rich CCAAT binding transcription factor involved in histone H1 cell cycle regulation
No full textH1TF2A: The large subunit of a heterodimeric glutamine rich CCAAT binding transcription factor involved in histone H1 cell cycle regulation
No full textH1TF2A: The large subunit of a heterodimeric glutamine rich CCAAT binding transcription factor involved in histone H1 cell cycle regulation
No full textIn vitro activity of six beta-lactam antibiotics against non-beta-lactamase producing and producing enterobacteria and antibiotic resistance transfer.
No full textbeta-Lactamase production was evaluated by chromogenic cephalosporin 87/312 in 184 enterobacteria isolated from clinical sources. Minimal inhibitory concentrations (MICs) of six beta-lactam antibiotics (Ampicillin, Cephaloridine, Cephalexine, Cefazoline, Cefuroxime, Cefotaxime) were determined on 90 non beta-lactamase producing and 94 beta-lactamase producing strains by a miniaturized dilution method. beta-lactamase production transfer and antibiotic resistance transfer were observed in a receiving E. coli K12 C600 NA- after conjugation with 94 producing strains. Cefotaxime showed high antibacterial activity both against beta-lactamase producing and non producing bacteria. Cefuroxime, Cephalexine and Cefazoline were active only against high percentage of non beta-lactamase producing bacteria. Ampicillin and Cephaloridine showed low antibacterial activity both against producing and non producing bacteria. About 19% (18/94) of beta-lactamase producing bacteria transferred beta-lactamase producing capacity to E. coli K12 by conjugation and a significant increase of MICs of each antibiotic, except that of Cefotaxime, was observed in E. coli K12 that acquired capacity to produce beta-lactamase by conjugation. Resistant strains that produce beta-lactamase transfer antibiotic resistance by conjugation to E. coli in variable percentages to the antibiotics under examination. Antibiotic resistance to Ampicillin, Cephaloridine, Cephalexine and Cefazolin observed in receiving strains after conjugation is not only due to beta-lactamase transfer. All strains acquiring Cefuroxime and Cefotaxime resistance never acquire beta-lactamase producing capacity
Gene expression profiles and molecular subtyping of hereditary haemochromatosis using cDNA microarray.
No full textShort and highly efficient synthetic promoters for melanoma-specific gene expression.
No full textHere, we report the construction and functional analysis of synthetic promoters designed for gene therapy applications requiring strong and specific gene expression in melanoma cell lines. We have analysed the transcriptional activity of different combinations of two transcriptional regulatory modules, a melanocyte-specific element from the human tyrosinase promoter and a cell-cycle-specific element from the human alpha-fetoprotein promoter. Transient expression assays in different cell lines show that several of these composite synthetic promoters can drive a strong and selective expression of a reporter gene in melanoma cell, providing us with a new powerful tool for gene therapy of melanomas
Short sequence motifs enriched in 5’ upstream and UTR regions of A.thaliana genes homologous to potato mRNAs hyper- or hypo-expressed upon hydric stress.
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