1,891 research outputs found
Di Gennaro A, Marini L, D’Ostilio A, Loreto MF, Modesti M, Scipione MS, Ginaldi L, De Martinis M, Quaglino D
No full textChanges in B cell antigen expressions in the elderly
No full textPO-0999 Changes in B cell antigen expression in the elderly
Ginaldi L, De Martinis M, D’Ostilio A, Marini L, Loreto MF, Profeta VF,*
Quaglino D
Department of Internal Medicine and Public Health, University of
L’Aquila, *Ser. T ASL Teramo, Italy
The involvement of the B cell compartment during senescence plays an
important role in the development of the immune dysfunction (e.g. hypergammaglobulinemia,
autoantibody production and impaired responses to
immunisations). In order to identify specific antigen expression changes on
B lymphocytes which may contribute to the immune deficiency in the elderly,
we investigated, by triple staining flow cytometry, the level of expression
of some constitutive surface markers (HLA-DR, CD19, CD20) and of a
series of adhesion molecules (CD49b, CD49d, CD50, CD62L) on B lymphocytes
from 23 healthy elderly individuals (82-100 years old, mean age
92) compared to 13 healthy young donors (20-50 years old, mean age
31). All aged donors fullfilled the admission criteria for gerontological studies
proposed by the Senicor Protocol. Both percentage and absolute number
of B lymphocytes (CD19+ and CD20+ cells) were significantly
decreased in the elderly compared to young donors. The absolute number
of B cells expressing the adhesion molecules CD49b and CD49d was lower
in elderly individuals, as well as the percentage value of B cells expressing
the adhesion molecules CD50 and CD62L. The percentage and
absolute number of B cells coexpressing the CD5 antigen was decreased
in elderly subjects. The CD20 expression antigen was increased on B lymphocytes
coexpressing the CD49b and CD49d in elderly donors compared
to young subjects. Also the CD5 density on B cells from old donors was
slightly increased compared to controls. No differences were detected in
the expression of CD19, CD50, CD49b, CD49d, CD62L and HLA-DR molecules
on B lymphocytes. Quantitative flow cytometry may be of value in
the elderly both for clinical and biological studies. The study of antigen density
changes on B cells in the elderly may allow a better understanding of
the humoral immune defects observed in these subjects and provide
insights into the functional defects of the B cell compartment characterising
immunosenescence
Strong Raman-induced noninstantaneous soliton interactions in gas-filled photonic crystal fibers
No full textWe have developed an analytical model based on the perturbation theory to study the optical propagation of two successive solitons in hollow-core photonic crystal fibers filled with Raman-active gases. Based on the time delay between the two solitons, we have found that the trailing soliton dynamics can experience unusual nonlinear phenomena, such as spectral and temporal soliton oscillations and transport toward the leading soliton. The overall dynamics can lead to a spatiotemporal modulation of the refractive index with a uniform temporal period and a uniform or chirped spatial period. (C) 2015 Optical Society of Americ
Rilevamento di differenti genotipi di Actinobacillus actinomycetemcomitans nel cavo orale (I classificato sessione poster)
No full text
RIC-HSCT for MF/SS
No full textAdvanced-stage mycosis fungoides and Sezary syndrome (MF/SS) have a poor prognosis. Allogeneic hematopoietic stem cell transplantation (HSCT), particularly using a reduced-intensity conditioning (RIC) regimen, is a promising treatment for advanced-stage MF/SS. We performed RIC-HSCT in nine patients with advanced MF/SS. With a median follow-up period of 954days after HSCT, the estimated 3-year overall survival was 85.7% (95% confidence interval, 33.4-97.9%) with no non-relapse mortality. Five patients relapsed after RIC-HSCT; however, in four patients whose relapse was detected only from the skin, persistent complete response was achieved in one patient, and the disease was manageable in other three patients by the tapering of immunosuppressants and donor lymphocyte infusion, suggesting that graft-versus-lymphoma effect and "down-staging" effect from advanced stage to early stage by HSCT improve the prognosis of advanced-stage MF/SS. These results suggest that RIC-HSCT is an effective treatment for advanced MF/SS
Linking shelfal 'turbidites' to their feeding system: the Monastero Fm. (eastern Tertiary Piedmont Basin)
No full textThough underappreciated in the sedimentary record, there is growing evidence that water- sediment mixtures generated at river mouths consequently to high-discharge events can transfer significant sediment volumes to deep-water via a range of flow types, referred to as hypo-homo-hyperpycnal flows. Identification of these deposits is difficult due to their resemblance to some classical turbidite facies, thus requiring accurate facies analysis.This work focuses on the facies analysis of a ca. 1100 m-thick pile of turbidites belonging to the Monastero Fm. (MF), which were deposited adjacent to coeval Gilbert-type fan-delta conglomerates (Savignone Conglomerates Fm.; SCF) as a part of the Rupelian-early Chattian fill of the eastern Tertiary Piedmont Basin (TPB; Northwest Italy).The MF consists of an apparently monotonous succession of thin-bedded sandstone-mudstone couplets with a sand/mud ratio >1 intercalated by erosive-based amalgamated beds-sets of sandstones and conglomerates a few-tens of metres-thick. The thin-bedded component of MF is dominant, representing about 70% of the total thickness of the studied section, and show a range of features which hardly fit into a turbidite depositional model. The majority of sandstone beds show erosional wavy bases and begins with either coarser basal lags or a thin inversely graded division, passing upward to two or more inversely-normally graded lamina sets separated each other by mud-drapes containing abundant phytoclasts. Typically, the top of these sandstone beds shows a variety of sedimentary structures (cross, trough-cross and hummocky lamination) and is capped by cm-thick heterolithics characterized by a range of flaser to lenticular bedding. In the lower part of MF, presence of symmetric ripple-marks ornamenting sandstone bed tops and articulated lamellibranch valves suggests deposition above the wave base level and short sediment routing.On the other hand, the thicker-bedded component of MF consists of amalgamated coarse-grained sandstones in which mud-draped scours, traction carpets, mud-chips and conglomeratic lags are suggestive of deposition from a range of hyper-concentrated to high-density flows transferring most of their load down-dip.The stratigraphic and lateral relationships of these two contrasting facies associations, along with their characters, suggest that the MF can be interpreted to reflect deposition in the pro-delta of the adjacent SCF system. In this view, the thin-bedded component of MF would represent the product of ‘background’ deposition by a range of hypo-homo-hyperpycnal flows generated at river mouths, whereas the coarser amalgamated counterpart likely represents pro-delta lobes
Flow cytometry in the study of acute leukaemias: II. Its role and relationship to other cytobiological techniques
No full textFlow cytometry has became an extremely
useful tool in thè diagnosis of hematological malignancies.
The multitude of available monoclonal antibodies
and thè routine use of multiparametric
techniques bave drastically improved thè diagnostic
utility of flow cytometry in thè study of leukemias.
This review focuses on thè use of flow cytometry in
thè routine clinicopathological approach to thè diagnosis
of acute leukemias. The Authors discuss not
only thè use of flow cytometry in thè differential diagnosis,
but also correlate flow cytometric immunophenotyping
with other cytobiologic techniques
in thè study of acute leukemia
- …
