1,721,007 research outputs found
GA‐RAGE – Effetti dell’albumina glicata plasmatica sulla stimolazione di RAGE nel diabete mellito di tipo 2 con complicanze renali
No full textIl ruolo della sieroalbumina umana glicata ﴾GA﴿ nella stimolazione dello stato ossidativo e proinfiammatorio mediato dal recettore RAGE è un aspetto dell’eziopatogenesi del diabete di tipo 2 ﴾DM‐2﴿ e delle sue complicanze croniche ancora da chiarire e ad alto potenziale di impatto sulla pratica clinica. Il progetto di ricerca GA‐RAGE si propone di sviluppare metodi analitici basati sulla combinazione di cromatografia di affinità, biosensori ottici e spettrometria di massa per lo studio della correlazione tra i livelli plasmatici di GA, l’attivazione del recettore RAGE ed il quadro clinico delle nefropatie diabetiche in una popolazione di pazienti affetti da DM‐2. La valutazione dell’interazione GA‐RAGE, effettuata ex vivo sui campioni plasmatici dei pazienti, permetterà di valutare: ﴾1﴿ l’efficacia di GA come indicatore per la prognosi e/o per la diagnosi precoce delle complicanze renali del DM‐2; ﴾2﴿ il potenziale ruolo dell’asse GA‐RAGE come bersaglio di nuove strategie terapeutiche
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Exopolysaccharides released by vaginal Lactobacillus strains: chemical characterization and modulation of biofilm formation.
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Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Redox-sensitive structural features of transketolase from Chlamydomonas reinhardtii (CrTK)
Full text linkSeveral studies identified the Calvin–Benson cycle (CBC), the photosynthetic pathway responsible for
carbon fixation (Figure 1), as a redox-regulated process. New biochemical and proteomic approaches
suggest that all the CBC enzymes may withstand redox regulation through multiple redox post-
translational modifications (PTMs), like disulfide bond formation, glutathionylation and nitrosylation 1 . This
redox control is likely involved in the adaptative response to environmental conditions, including
light/dark transitions and abiotic/biotic stress.
This ongoing work aims at investigating the redox regulation of the chloroplastic CBC enzyme
transketolase from the green microalga Chlamydomonas reinhardtii (CrTK). Recombinant CrTK was
heterologously expressed in E. coli and purified to homogeneity through metal affinity chromatography.
In vitro activity assays showed that the purified enzyme displays a redox-sensitivity being partially
inhibited by oxidizing treatments. Moreover, the 3D-structure of the reduced protein (solved at 1.8 Å
resolution) revealed the presence of several cysteine couples located at suitable distance for disulfide
bond formation and in close proximity to catalytic residues. The influence of the redox state on the
structural features of CrTK was further investigated by LC-ESI-MS/MS analysis, allowing the identification
of the regulatory cysteines. Concomitantly, circular dichroism (CD) analysis in the far-UV spectral region
allowed evaluating redox-dependent changes in the secondary structure of the enzyme. Furthermore, CD
analysis in the near-UV region showed the onset of an induced circular dichroism (ICD) signal 3 arising from
the interaction between CrTK and the cofactor thiamine pyrophosphate (TPP), which was exploited to
monitor CrTK-TPP binding under different redox states of the enzyme
Interaction between commercial glycated HSA and the extracellular region of human RAGE: a SPR-based study
No full textAppropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
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